Apoptosis induced by membrane damage in human lymphocytes; effects of arachidonic acid and its photoproducts

• Autorzy: Zarebska Z. , Zielinska J. , Zhukov I. , Maslinski W.
• Języki publikacji: EN

Abstrakty

EN

The effect of arachidonic acid (AA) combined with UVA irradiation was studied in a model system mimicking phototherapy PUVA (psoralen+UVA) ex vivo in vitro. The contribution of damage to the plasma membrane by PUVA was tested on human lymphocytes derived from healthy donors. The effect of arachidonic acid (AA) combined with UVA irradiation was compared with that of a psoralen photoadduct to AA added to the culture. The adduct, obtained photochemically and purified, was characterized by NMR and MS spectrometry as a cycloadduct of psoralen to the vinylene bond of the acid (AA<>PSO).  The reactions of cultured cells, manifested 20 h after treatment by changes in apoptosis and mitochondrial depolarization, were monitored by flow cytometry by tagging lymphocytes with appropriate fluorescent probes. Treatment of lymphocyte suspension within AA doses from 40 to 100 μM gradually induced a shift from Anx-V+ (single positive cells) to late apoptotic, Anx-V+PI+ (double positive cells) in a dose dependent manner. The adduct, AA<>PSO, induced apoptotic changes at a concentration 2–3 times higher than free AA. Combination of psoralen (1 μM ) or arachidonic acid (20–120 μM) with UVA irradiation (2–6 J/cm2) accelerated the plasma membrane changes in a synergic way. Preliminary studies indicated that changes in the transmembrane potential of mitochondria paralleled the apoptosis when cells were treated by AA alone.  Our findings showed that UVA radiation of lymphocytes in the presence of arachidonic acid, as in the presence of psoralen, enhanced apoptosis of cells in a synergic manner. Thus, PUVA-induced apoptosis may proceed in part by a still undefined signaling pathway(s) triggered in lymphocyte membranes.

Słowa kluczowe

EN

plasma membrane; arachidonic acid; photochemotherapy; psoralen; membrane damage; man; human lymphocyte; lymphocyte; propidium iodide; apoptosis; photoproduct

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2005
• Tom: 52
• Numer: 1
• Opis fizyczny: p.179-194,fig.,ref.

Twórcy

autor

Zarebska Z.

• Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland

autor

Zielinska J.

autor

Zhukov I.

autor

Maslinski W.

Bibliografia

• Bax A, Davis DG. (1985) MLEV-17 based two-dimensional homonuclear magnetization transfer spectroscopy. J Magn Reson.; 65: 355–60.
• Berger CL, Hanlon D, Kanada D, Girardi M, Edelson RL. (2002) Transimmunization, a novel approach for tumor immunotherapy. Transfus Apheresis Sci.; 26: 205–16.
• Bernardi P, Penzo D, Wojtczak L. (2002) Mitochondrial energy dissipation by fatty acids. Mechanisms and implications for cell death. Vitam Horm.; 65: 97–126.
• Bladon J, Taylor PC. (2003) Treatment of cutaneous T cell lymphoma with extracorporeal photopheresis induces Fas-ligand expression on treated T cells, but does not suppress the expression of co-stimulatory molecules on monocytes. J Photochem Photobiol B: Biol.; 69: 129–38.
• Caffieri S, Zarębska Z, Dall’Acqua F. (1991) Membrane lymphocyte damage by 8 methoxy-psoralen and UVA radiation. Méd Biol Environ.; 19: 45–50.
• Caffieri S, Zarębska Z, Dall’Acqua F. (1996) Psoralen photosensitization: damages to nucleic acid and membrane lipid components. Acta Biochim Polon.; 43: 241–6.
• Calder PC, Bond JA, Bevan SJ, Hunt SV, Newsholme EA. (1991) Effect of fatty acids on the proliferation of concanavalin A-stimulated rat lymph node lymphocytes. Int J Biochem.; 23: 579–88.
• Calzavara-Pinton PG. (1997) Efficacy and safety of stand-up irradiation cubicles with UVA metal-halide lamps (and a new filter) or UVA fluorescent lamps for photochemotherapy of psoriasis. Dermatology.; 195: 243–7.
• Canton M, Caffieri S, Dall’Acqua F, Di Lisa F. (2002) PUVA-induced apoptosis involves mitochondrial dysfunction caused by the opening of the permeability transition pore. FEBS Lett.; 522: 168–72.
• Cohen D, De Leo VA. (1993) Ultraviolet radiation- induced phospholipase A2 activation occurs in mammalian cell membrane preparations. Photochem Photobiol.; 57: 383–90.
• Coven TR, Walters IB, Cardinale I, Krueger JG. (1999) PUVA-induced lymphocyte apoptosis: mechanism of action in psoriasis. Photodermatol Photoimmunol Photomed.; 15: 22–7.
• Enomoto DNH, Schellekenes PTA, Yong S-L, ten Berge IJM, Mekkes JR, Bos JD. (1997) Extracorporeal photochemotherapy (photopheresis) induces apoptosis in lymphocytes: a possible mechanism of action of PUVA therapy. Photochem Photobiol.; 65: 177–80.
• Frank S, Caffieri S, Raffaelli A, Vedaldi D, Dall’Acqua F. (1998) Characterisation of psoralen-oleic acid cycloadducts and their possible involvement in membrane photodamage. J Photochem Photobiol B: Biol.; 44: 39–44.
• Godar DE. (1999a) Light and death: photons and apoptosis. J Invest Dermatol Proc.; 4: 17–23.
• Godar DE. (1999b) UVA1 radiation triggers two different final apoptotic pathways. J Invest Derm.; 112: 3–12.
• Gordon PM, Diffey BL, Matthews JN, Farr PM. (1999) A randomized comparison of narrow band TL-01 phototherapy and PUVA photochemotherapy for psoriasis. J Am Acad Dermatol.; 41: 728–32.
• Higuchi Y, Yoshimoto T. (2002) Arachidonicm acid converts the glutathione depletion-induced apoptosis to necrosis by promoting lipid peroxidation and reducing caspase-3 activity in rat glioma cells. Arch Biochem Biophys.; 400: 133–40.
• Krutmann J. (1998) Therapeutic photoimmunology: photoimmunological mechanisms in photo(chemo)therapy. J Photochem Photobiol B: Biol.; 44: 159–64.
• Nath A. (2001) Apoptosis in HIV disease pathogenesis. In Programmed Cell Death II.
• Role in Disease, Pathogenesis and Prevention. Mattson MP, Estus S, Rangnekar VM, eds. pp 101–36. Elsevier Science, Amsterdam.
• Nghiem DX, Walterscheid JP, Kazimi N, Ullrich SE. (2002) Ultraviolet radiation-induced immunosuppression of delayed-type hypersensitivity in mice. Methods.; 28: 25–33.
• Pathak MA, Fitzpatrick TB. (1992) The evolution of photochemotherapy with psoralens and UVA (PUVA): 2000 BC to 1992 AD. J Photochem Photobiol B: Biol.; 14: 3–22.
• Penzo D, Petronilli V, Angelin A, Cusan C, Colonna R, Scoranno L, Pagano F, Prato M, Di Lisa F, Bernardi P. (2004) Arachidonic acid released by phospholipase A2 activation triggers Ca2+-dependent apoptosis through the mitochondrial pathway. J Biol Chem.; 279: 25219–25.
• Pompéia C, Freitas JJS, Kim JS, Zyngier SB, Curi R. (2002) Arachidonic acid cytotoxicity in leukocytes: implications of oxidative stress and eicosanoid synthesis. Biol Cell.; 94: 251–65.
• Punnonen K, Jansen CT, Puntala A, Ahotupa M. (1991) Effects of in vitro UVA irradiation and PUVA treatment on membrane fatty acids and activities of antioxidant enzymes in human keratinocytes. J Invest Dermatol.; 96: 255–9.
• Schindl A, Klosner G, Hönigsmann H, Jori G, Calzavara-Pinton PG, Trautinger F. (1998) Flow cytometric quantification of UV-induced cell death in a human squamous cell carcinoma- derived cell line: dose and kinetic studies. J Photochem Photobiol B: Biol.; 44: 97–106.
• Schmitt IM, Chimenti S, Gasparro FP. (1995) Psoralen-protein photochemistry — a forgotten field. J Photochem Photobiol B: Biol.; 27: 101–7.
• Song PS, Tapley KJ. (1979) Photochemistry and photobiology of psoralens. Photochem Photobiol.; 29: 1177–97.
• Tokura Y, Seo N, Yagi H, Takigawa M. (2001) Photoactivational cytokine-modulatory action of 8-methoxypsoralen plus ultraviolet A in lymphocytes, monocytes, and cutaneous T cell lymphoma cells. Ann N Y Acad Sci USA.; 941: 185–93.
• Vento R, D’Alessandro N, Giuliano M, Lauricella M, Carabill M, Tesoriere G. (2000) Induction of apoptosis by arachidonic acid in human retinoblastoma Y79 cells: involvement of oxidative stress. Exp Eye Res.; 70: 503–17.
• Walterscheid JP, Ullrich SE, Nghiem DX. (2002) Platelet-activating factor, a molecular sensor for cellular damage, activates systemic immune suppression. J Exp Med.; 195: 171–9.
• Waszkowska E, Zarêbska Z, Poznañski J, Zhukov I. (2000) Spectroscopic detection of photoproducts in lecithin model system after 8-methoxypsoralen plus UV-A treatment. J Photochem Photobiol B: Biol.; 55: 145–54.
• Wiswedel I, Bohne M, Hirsch D, Kuhn H, Augustin W, Gollnick H. (2000) A sensitive gas chromatography-mass spectrometry assay reveals increased level of monohydroxyeicosatetraenoic acid isomers in human plasma after extracorporeal photoimmunotherapy and under in vitro ultraviolet A exposure. J Invest Dermatol.; 115: 499–503.
• Wolf LA, Laster SM. (1999) Characterization of arachidonic acid-induced apoptosis. Cell Biochem Biophys.; 30: 353–68.
• Wolnicka-Głubisz A, Rijnkels JM, Sarna T, Beijersbergen van Henegouwen GMJ. (2002) Apoptosis in leukocytes induced by UVA in the presence of 8-methoxypsoralen, chlorpromazine or 4,6,4-trimethylangelicin. J Photochem Photobiol B: Biol.; 68: 65–72.
• Zarębska Z, Waszkowska E, Caffieri S, Dall’Acqua F. (1998) Photoreactions of psoralens with lecithins. J Photochem Photobiol B: Biol.; 45: 122–30.
• Zarębska Z, Waszkowska E, Caffieri S, Dall’Acqua F. (2000) PUVA (psoralen+UVA) photochemotherapy: processes triggered in the cells. Il Farmaco.; 55: 515–20.