PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2010 | 66 | 05 |

Tytuł artykułu

Jersinioza ryb lososiowatych - charakterystyka czynnika etiologicznego i metody jego identyfikacji

Warianty tytułu

EN
Yersiniosis of salmonids: characteristic etiological factors and methods of its identification

Języki publikacji

PL

Abstrakty

EN
Yersinia ruckeri, a Gram-negative rod belonging to Enterobacteriaceae family, genus Yersinia, is a causative agent of yersiniosis of salmonids. The size of the active growth cells are 0.75 × 1.0 µm - 3 µm. The presence of flagella are connected with motile ability but non motile bacterium are isolated more frequently. The biochemical characteristics of Y. ruckeri strains are rather homogenous. Y. ruckeri rods in in vitro examination are characterized as quite sensitive to medicines. All of the Polish isolates were sensitive to oxytetracycline, flumeqine and enrofloxacin. Classical bacteriological methods are mainly used for the diagnostics of yersinisis. Tryptone soya agar or 5% blood agar are carried out for the isolation of the microorganism. Selective and facultative mediums such as Furones medium, ROD or Waltman-Shotts media are also used. Identification of the isolated strains is performed by the characteristics of their biochemical properties, after which an analysis of the obtained profiles is done. Commercial API 20E kits are used in the examination of biochemical characteristic of the isolates but it is necessary for the results of the identification to be always supplemented by complementary tests. The plate agglutination test and PCR are carried out as a confirmation of identification of Y. ruckeri rods. This last method, on account of its high sensitivity, is useful in the detection of asymptomatic carriers of bacterium Y. ruckeri.

Wydawca

-

Rocznik

Tom

66

Numer

05

Opis fizyczny

s.299-301,rys.,tab.,bibliogr.

Twórcy

autor
  • Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy

Bibliografia

  • 1. Altinok I., Grizzle J. M., Liu Z.: Detection of Yersinia ruckeri in rainbow trout blood by the polymerase chain reaction. Dis. Aquat. Org. 2001, 44, 29-34.
  • 2. Argenton F., De Mas S., Malocco C., Dalla Valle L., Giorgetti G., Colombo L.: Use of random DNA amplification to generate specific molecular probes for hybridization tests and PCR-based diagnosis of Yersinia ruckeri. Dis. Aquat. Org. 1996, 24, 121-127.
  • 3. Austin B., Austin A.: Bacterial fish pathogens. Diseases of fish farmed and wild fish. Praxis Publishing, Chichester, UK 2007, 111-112.
  • 4. Bottone E. J., Bercovier H., Mollaret H. H.: Genus. XLI. Yersinia van Loghem 1944, 15AL, [w:] Garrity G. M., Brenner D. J., Krieg N. R., Staley J. T. (wyd.): Bergey's Manual of Systematic Bacteriology. t. 2, cz. B, Springer-Verlag, New York 2005, 838-848.
  • 5. Bullock G. L., Maestrone G., Starliper C., Schill B.: Potentiated sulfonamide therapy of enteric redmouth disease. Can. J. Fish. Aquat. Sci. 1983, 40, 101-102.
  • 6. Ceschia G., Giorgetti G., Bertoldini G., Fontebasso S.: The in vitro sensivity of Yersinia ruckeri to specific antibiotics. J. Fish Dis. 1987, 10, 65-67.
  • 7. Daly J. G., Lindvik B., Stevenson R. M. W.: Serological heterogeneity of recent isolates of Yersinia ruckeri from Ontario and British Columbia. Dis. Aquat. Org. 1986, 1, 151-153.
  • 8. Davies R. L., Frerichs G. N.: Morphological and biochemical differences among isolates of Yersinia ruckeri obtained from wide geographical areas. J. Fish Dis. 1989, 12, 357-365.
  • 9. Ewing E. W., Ross A. J., Brenner D. J., Fanning G. R.: Yersinia ruckeri sp. nov., the redmouth (RM) bacterium. Int. J. Syst. Bacteriol. 1978, 28, 37-44.
  • 10. Furones M. D., Gilpin M. L., Munn C. B.: Culture media for the differentiation of isolate Yersinia ruckeri, based on detection of a virulence factor. J. Appl. Bacteriol. 1993, 74, 360-366.
  • 11. Gibello A., Blanco M. M., Moreno M. A., Cutuli M. T., Doménech A., Dominguez L., Fernández-Garayzábal J. F.: Development of PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout. Appl. Environm. Microbiol. 1999, 65, 346-350.
  • 12. Grandis S. A. de, Krell P. J., Flett D. E., Stevenson R. M. W.: Deoxyribonucleic acid relatedness of serovars of Yersinia ruckeri, the enteric redmouth bacterium. Int. J. Syst. Bacteriol. 1988, 38, 4995-5008.
  • 13. Green M., Austin B.: The identification of Yersinia ruckeri and its relationship to other representatives of the Enterobactericeae. Aquaculture 1982, 34, 185-192.
  • 14. Horne M. T., Barnes A. C.: Enteric redmouth disease (Yersinia ruckeri), [w:] Woo P. T. K., Bruno D. W. (wyd.): Fish diseases and disorders, viral, bacterial and fungal infections. T. 3, Cabi Publishing, Wallingford 1999, 455-477.
  • 15. Ibrahim A., Goebel B. M., Liesack W., Griffiths M., Stackebrandt E.: The phylogeny of the genus Yersinia based on 16S rDNA sequences. FEMS Microbiol. Lett. 1993, 114, 173-178.
  • 16. Klein B. U., Kleingeld D. W., Böhm K. H.: First isolation of a non-motile/Tween 80 negative Yersinia ruckeri strain in Germany. Bull. Europ. Ass. Fish Pathol. 1994, 14, 165-166.
  • 17. Kozińska A., Guz L., Pękala A.: Diagnostyka wybranych patogenów bakteryjnych w ichtiopatologii. Państwowy Instytut Weterynaryjny 2002, 66-71.
  • 18. Kozińska A., Pękala A.: Występowanie Yersinia ruckeri u ryb łososiowatych w Polsce i fenotypowa charakterystyka izolatów. Medycyna Wet. 2004, 60, 880-882.
  • 19. O'Leary P. J.: Enteric redmouth of salmonids: A biochemical and serological comparison of selected isolates. Praca magisterska, Oregon State University 1977.
  • 20. Pękala A.: Bakterie najczęściej izolowane od pstrągów i ich wrażliwość na wybrane chemioterapeutyki. XII Kongres PTNW, Wydawnictwo SGGW, Warszawa 2004, s. 402.
  • 21. Pękala A.: Charakterystyka fenotypowa i genotypowa krajowych izolatów Yersinia ruckeri w aspekcie ich patogenności dla ryb. Praca doktorska, Zakład Chorób Ryb, PIWet-PIB, Puławy 2008.
  • 22. Rigos G., Stevenson R.: The effect of antibiotic treatment on the establishment of persistent infection with Yersinia ruckeri Serovar II in rainbow trout Oncorhynchus mykiss (Walbaum). Aquacult. Internat. 2001, 9, 247-253.
  • 23. Rodgers C. J.: Development of a selective-differential medium for the isolation of Yersinia ruckeri and its application in epidemiological studies. J. Fish Dis. 1992, 15, 243-254.
  • 24. Ross A. J., Rucker R. R., Ewing W. H.: Description of a bacterium associated with redmouth diseases of rainbow trout (Salmo gairdneri). Can. J. Microbiol. 1966, 12, 763-770.
  • 25. Rucker R. R.: Redmouth disease of rainbow trout (Salmo gairdneri). Bull. Internat. Off. Epizoot. 1966, 65, 825-830.
  • 26. Sakai T., Oseko N., Iida T.: Rapid and simple method for the detection of Yersinia ruckeri, the causal agent of enteric redmouth disease. Fish Pathol. 2006, 41, 127-130.
  • 27. Stevenson R. M. W., Airdrie D. E.: Serological variation among Yersinia ruckeri strains. J. Fish Dis. 1984, 7, 247-254.
  • 28. Stevenson R. M. W., Flett D., Raymond B. T.: Enteric redmouth (ERM) and other enterobacterial infections of fish. Rozdzia³ 5, [w:] Ingis V., Roberts R. J., Bromage N. R. (wyd.) Bacterial Diseases of Fish. Blackwell Scientific Publications, Oxford 1993, 80-105.
  • 29. Taylor P. W., Winton J. R.: Optimization of nested polymerase chain reaction assay for identification of Aeromonas salmonicida, Yersinia ruckeri and Flavobacterium psychrophilum. J. Aquat. Anim. Health 2002, 14, 216-224.
  • 30. Treves-Brown K. M.: Applied Fish Pharmacology. Kluwer Academic Publisher, Dordrecht, Holandia 2000.
  • 31. Waltman W. D., Shotts E. B.: A medium for the isolation and differentiation of Yersinia ruckeri. Can. J. Fish. Aquat. Sci. 1984, 41, 804-806.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-e0e26b52-54f6-4073-b898-9b6aa754fa1b
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.