Wykorzystanie metody hemi-nested RT-PCR do roznicowania zakazen wywolanych klasycznym wirusem wscieklizny i EBLV 1 u zwierzat ladowych

• Autorzy: Smreczak M , Orlowska A. , Trebas P. , Zmudzinski J.F.

Warianty tytułu

EN

Application of hemi-nested RT-PCR method for differentiation of classical rabies and EBLV 1 infection in terrestrial animals

• Języki publikacji: PL

Abstrakty

EN

Currently in the routine diagnosis of rabies three techniques are recommended: the florescent antibody test, mouse inoculation test and tissue culture inoculation test. Unfortunately these methods do not allow us to differentiate the classical rabies virus from an EBLV 1 infection in terrestrial animals. Such a possibility is provided by using hemi-nested RT-PCR with specific primers. Conditions of the hnRT-PCR method have been previously established. It makes it possible to detect genetic material both of lyssavirus genotypes as well as to establish a differentiation between genotypes. Application of the hnRT-PCR method for rabies discrimination between two genotypes allows for an easier and more reliable diagnosis of rabies in terrestrial animals.

Słowa kluczowe

PL

wirus wscieklizny; wirus wscieklizny nietoperzy europejskich; infekcja wirusowa; wirus EBLV1 zob.wirus wscieklizny nietoperzy europejskich; metoda hemi-nested RT-PCR; wscieklizna; zwierzeta; lyssawirusy; choroby zwierzat; roznicowanie; metody

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 03
• Opis fizyczny: s.314-317,fot.,bibliogr.

Twórcy

autor

Smreczak M

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy

autor

Orlowska A.

autor

Trebas P.

autor

Zmudzinski J.F.

Bibliografia

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