Survivin is a key factor in the differential susceptibility of gastric endothelial and epithelial cells to alcohol-induced injury

• Autorzy: Jones M. K. , Padilla O.R. , Zhu E.
• Języki publikacji: EN

Abstrakty

EN

We previously demonstrated that the anti-apoptosis protein, survivin, plays a protective role against alcohol-induced gastric injury. Since the endothelium is a primary target of alcohol-induced gastric damage, we investigated whether survivin expression is a key factor in the greater susceptibility of gastric endothelial vs. epithelial cells to alcohol-induced injury. Here, we demonstrate that rat gastric epithelial cells (RGM1 cells, an epithelial cell line derived from normal rat gastric mucosa) expressed 7.5-fold greater survivin protein levels vs. rat gastric endothelial cells. Survivin expression correlated with resistance of gastric epithelial vs. endothelial cells to both alcohol-induced cell damage and alcohol-induced apoptosis. Suppression of survivin protein expression levels using siRNA rendered the gastric epithelial cells as susceptible to both alcohol-induced cell damage and apoptosis as the gastric endothelial cells. Conversely, forced overexpression of survivin by transient transfection rendered gastric endothelial cells as resistant to both alcohol-induced cell damage and apoptosis as mock-transfected gastric epithelial cells. Moreover, overexpression of a threonine-34 to glutamate phosphorylation mimic mutant survivin construct rendered gastric endothelial cells significantly more resistant to alcohol-induced damage and apoptosis vs. mock-transfected gastric epithelial cells. These findings indicate that disparate survivin expression levels can explain the discrepancy between gastric epithelial and endothelial cell susceptibility to alcohol-induced injury; and, that a negative charge at amino acid residue 34 on survivin, such as that which naturally occurs by phosphorylation of threonine-34, enhances its property in conferring gastric mucosal protection.

Słowa kluczowe

EN

survivin; gastric endothelial cell; epithelial cell; alcohol-induced injury; antiapoptotic protein; gastric mucosa; rat; apoptosis; cell death; in vitro; mutagenesis; overexpression; phosphorylation; transfection

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2010
• Tom: 61
• Numer: 3
• Opis fizyczny: p.253-264,fig.,ref.

Twórcy

autor

Jones M. K.

• Research Healthcare Group, VA Long Beach Healthcare System, 5901 E. Seventh Street, Long Beach, CA 90822 USA

autor

Padilla O.R.

autor

Zhu E.

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