Characteristics of genes encoding structural protein of Polish strains of goose parvovirus

• Autorzy: Wozniakowski G , Samorek-Salamonowicz E. , Kozdrun W.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to come up with molecular characteristics of Polish strains of goose parvovirus (GPV) on the basis of genes encoding their structural proteins. Ten field GPV strains and two vaccine strains: B-38 and MFP were used. The PCR- RFLP method based on three distinct endonucleases: HinfI, MboI, and RsaI, which had restriction sites within the sequences of examined genes, was applied. It was found that the homology of VP1, VP2, and VP3 among the studied strains ranged from 50% to 100%. The major differences in restriction patterns were found after application of HinfI, whereas 100% homology was observed after Rsal digestion. Significant differences were observed in restriction profiles of vaccine GPV strains. The study revealed that the application of PCR-RFLP for the analysis of VP1, VP2, and VP3 genes allowed for their molecular characteristics and differentiation between the vaccine and field strains.

Słowa kluczowe

PL

animal disease; Derzsy's disease; goose; parvovirus; restriction analysis; Polish strain; goose parvovirus; gene encoding; structural protein; VP1 gene; VP2 gene; VP3 gene

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 2
• Opis fizyczny: p.119-122,fig.,ref.

Twórcy

autor

Wozniakowski G

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Samorek-Salamonowicz E.

autor

Kozdrun W.

Bibliografia

• 1. Andreasen J.R., Glisson J.R., Villegas P.: Differentiation of vaccine strains and Georgia field isolates of infectious laryngotracheitis virus by their restriction endonuclease fragment patterns. Avian Dis 1990, 34, 646-656.
• 2. Chang P.C., Shien J.H., Wang M.S., Shieh H.K.: Phylogenetic analysis of parvoviruses isolated in Taiwan from ducks and geese. Avian Pathol 2000, 29, 45-49.
• 3. Cotmore S.F., Tattersall P.: Structure and organization of the viral genome. In: Parvoviruses, edited by Kerr J.R., Cotmore S.F., Bloom M.E., Linden R.M., Parrish C.R., Oxford University Press, Oxford, 2006, pp. 73-94.
• 4. Kozdruń W., Mato T., Palya V., Samorek-Salamonowicz E., Król K., Woźniakowski G.: Phylogenetic analysis of Derzsy's disease virus isolated from geese in Poland. Medycyna Wet 2008, 64, 961-1072.
• 5. Kataria R.S., Tiwari A.K., Butchaiah G., Kataria J.M.: Differentiation of infectious bursal disease virus strains by restriction analysis of RT-PCR-amplified VP2 gene sequences. Acta Virol 1999, 43, 245-249.
• 6. Kirkpatrick N.C., Mahmoudian A., O'Rourke D., Noormohammadi A.H.: Differentiation of infectious laryngotracheitis virus isolates by restriction fragment length polymorphic analysis of polymerase chain reaction products amplified from multiple genes. Avian Dis 2006, 50, 28-33.
• 7. Nanthakumar T., Kataria R.S., Tiwari A.K., Butchaiah G., Kataria J.M.: Pathotyping of Newcastle disease viruses by RT-PCR and restriction enzyme analysis. Vet Res Comm 2000, 24, 275-286.
• 8. Sirivan P., Obayashi M., Nakamura M.: Detection of goose and Muscovy duck parvoviruses using polymerase chain reaction-restriction enzyme length polymorphism analysis. Avian Dis 1998, 42, 133-139.
• 9. Tatár-Kis T., Mató T., Markos B., Palya V.: Phylogenetic analysis of Hungarian goose parvovirus isolates and vaccine strains. Avian Pathol 2004, 33, 438-444.
• 10. Toroghi R., Kataria J.M., Balamurugan V.: Differentiation of classical and very virulent strains/isolates of infectious bursal disease virus by reverse transcription-polymerase chain reaction. Acta Virol 2003, 47, 259-263.
• 11. Woźniakowski G., Kozdruń W., Samorek-Salamonowicz E.: Genetic variance of Derzsy's disease strains isolated in Poland. J Mol Gen Med 2009, 3, 210-216.
• 12. Zádori Z., Erdei J., Nagy J., Kisary J.: Characteristics of the genome of goose parvovirus. Avian Pathol 1994, 23, 359-364.
• 13. Zádori Z., Stefancsik R., Rauch T., Kisary J.: Analysis of complete nucleotide sequences of goose and Muscovy duck parvoviruses indicates common ancestral origin with adeno-associated virus 2. Virology 1995, 212, 562-573.