Detection of serum Pneumocystis carinii DNA in the course of P.carinii pneumonia in experimental animals and HIV-infected patients

• Autorzy: Golab E , Sobolewska A , Bitkowska E , Bednarska A , Berak H , Dzbenski T H
• Języki publikacji: EN

Abstrakty

EN

A nested PCR which amplified a part of the mitochondrial gene encoding the large subunit of rRNA of Pneumocystis carinii was used to detect P. carinii DNA in sera obtained from HIV-infected patients and immunosuppressed rats. P. carinii DNA was found in sera from nearly 52% of rats between 6th and 9th week of immunosuppressive treatment and in 12 serum samples out of 51 collected from HIV-infected patients, including 4 from clinically suspected and laboratory confirmed cases of PCP. The results of the studies indicated that DNA of P. carinii is most likely to appear in the blood in the advanced infection, however, the test for serum DNA may be of little value for the diagnosis of PCP since negative results do not exclude active infections and positive ones may be encountered in asymptomatic carriers.

Słowa kluczowe

EN

Pneumocystis carinii; rRNA; patient; infection; serum; laboratory diagnosis; AIDS; pneumonia; HIV; polymerase chain reaction; experimental animal; DNA

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2002
• Tom: 47
• Numer: 3
• Opis fizyczny: p.235-238

Twórcy

autor

Golab E

• Department of Medical Parasitology, National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland

autor

Sobolewska A

• Department of Medical Parasitology, National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland

autor

Bitkowska E

• Department of Medical Parasitology, National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland

autor

Bednarska A

• Hospital for Infectious Diseases, Wolska 37, 01-201 Warszawa, Poland

autor

Berak H

• Hospital for Infectious Diseases, Wolska 37, 01-201 Warszawa, Poland

autor

Dzbenski T H

• Department of Medical Parasitology, National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland

Bibliografia

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