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2007 | 63 | 01 |

Tytuł artykułu

Wplyw beta-1,3-1,6-D-glukanu na nieswoiste komorkowe mechanizmy obronne jagniat

Warianty tytułu

EN
Influence of beta-1,3-1,6-D-glucan on non-specific cellular defence mechanisms in lambs

Języki publikacji

PL

Abstrakty

EN
The aim of the study was to estimate the influence of β-1,3/1,6-D-glucan applied in feed on non-specific cellular immunity in lambs. The study examined twenty six 21-28 day old lambs in two groups: I - control and II - experimental (7 rams and 6 ewes in each group). The animals were kept under identical zoohygienic and nutritional conditions. Lambs from group I were fed a basal control diet and lambs from group II were fed a diet containing β-1,3/1,6-D-glucan, at doses of 80 mg/kg/day. Blood was taken from the control and experimental groups before the lambs were fed a diet containing β-1,3/1,6-D-glucan, and 15, 30 and 60 days after administration of the diet. The respiratory burst activity (RBA) and potential killing activity (PKA) of blood phagocytes and proliferative response of blood lymphocytes (MTT) stimulated by mitogens concanavalin A (ConA) and lipopolisaccharide (LPS) were examined. The results indicated that when β-1,3/1,6-D-glucan was applied to food it statistically significantly increased the blood phagocyte and lymphocyte activity in the lambs until the end of experiment, compared to the control group.

Wydawca

-

Rocznik

Tom

63

Numer

01

Opis fizyczny

s.84-86,rys.,bibliogr.

Twórcy

autor
  • Uniwersytet Warminsko-Mazurski, ul.Oczapowskiego 9, 10-957 Olsztyn
autor

Bibliografia

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  • 3. Buddle B. M., Pulford H. D., Ralston M.: Protective effect of glucan against experimentally induced staphylococcal mastitis in ewes. Vet. Microbiol. 1988, 16, 67-76.
  • 4. Chang C. F., Chen H. Y., Su M. S., Liao I. C.: Immunomodulation by dietary beta-1,3-glucan in the brooders of the black tiger shrimp Penaeus monodon. Fish Shellfish Immunol. 2000, 10, 505-514.
  • 5. Chung S., Secombes S. J.: Analysis of events occurring within teleost macrophages during the respiratory burst. Comp. Biochem. Physiol. 1988, 89B, 539-544.
  • 6. Hashimoto K., Suzuki I., Yadomae T.: Oral administration of SSG, a beta-glucan obtained from Sclerotinia sclerotiorum, affects the function of Peyer's patch cells. Int. J. Immunopharmacol. 1991, 13, 437-442.
  • 7. Kim H. M., Han S. B., Oh G. T., Kim Y. H., Hong D. H., Hong N. D., Yoo I. D.: Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. Int. J. Immunopharmacol. 1996, 18, 295-303.
  • 8. Li J., Xing J., Li D., Wang X., Zhao L., Lv S., Huang D.: Effects of beta-glucan extracted from Saccharomyces cerevisiae on humoral and cellular immunity in weaned piglets. Arch. Anim. Nutr. 2005, 59, 303-312.
  • 9. Milewski, S., Wójcik R., Małaczewska J., Trapkowska S., Siwicki A. K.: Wpływ β-1,3/1,6-D-glukanu na cechy użytkowości mięsnej oraz nieswoiste humoralne mechanizmy obronne jagniąt. Medycyna Wet. (w druku).
  • 10. Mosmann T.: Rapidcolorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65, 55-63.
  • 11. Nartowska J., Skopińska-Różewska E., Siwicki A. K.: Nowe immunomodulatory pochodzenia roślinnego, [w:] Siwicki A. K., Skopińska-Różewska E., Świderski F. (red.): Immunomodulacja - nowe możliwości w ochronie zdrowia. Olsztyn, Edycja 2004, s. 7-21.
  • 12. Ohno N., Egawa Y., Hashimoto T., Adachi Y., Yadomae T.: Effect of beta-glucans on the nitric oxide synthesis by peritoneal macrophage in mice. Biol. Pharm. Bull. 1996, 19, 608-612.
  • 13. Pelizon A. C., Kaneno R., Soares A. M., Meira D. A., Satori A.: Immunomodulatory activities associated with beta-glucan derived from Saccharomyces cerevisiae. Physiol. Res. 2005, 54, 557-564.
  • 14. Ríos-Hernández M., Dos-Santos N. J., Silvia-Cardoso, Bello-Gárciga J. L., Pedroso M.: Immunopharmacological studies of beta-1,3-glucan. Arch. Med. Res. 1994, 25, 179-180.
  • 15. Rook G. A., Steele W., Umar J., Dockrell H. M.: A simple method for the solubilization of reduced NBT and its use as a colorimetricassay for activation of human macrophages by gamma interferon. J. Immunol. Meth. 1995, 82, 161-167.
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  • 18. Sakurai T., Ohno N., Yadomae T.: Intravenously administered (1-3)-beta-D-glucan, SSG, obtained from Sclerotinia sclerotiorum IFO 9395 augments murine peritoneal macrophage functions in vivo. Chem. Pharm. Bull. 1992, 40, 2120-2124.
  • 19. Siwicki A. K., Kazuń K., Głąbski E., Terech-Majewska E., Baranowski P., Trapkowska S.: The effect of beta-1.3/1.6-glucan in diets on the effectiveness of anti-Yersinia ruckeri vaccine - an experimental study in rainbow trout (Oncorhynchus mykiss). Pol. J. Food Nutr. Sci. 2004, 54, 59-61.
  • 20. Siwicki A. K., Klein P., Morand M.: Immunomodulacja - aktualny stan wiedzy, [w:] Siwicki A. K. (red.): Wpływ ksenobiotyków na organizm zwierząt i człowieka. Olsztyn, IRS 1999, 93-102.
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  • 22. Suzuki I., Tanaka H., Kinoshita A., Oikawa S., Osawa M., Yadomae T.: Effect of orally administered beta-glucan on macrophage function In mice. Int. J. Immunopharmacol. 1990, 12, 675-684.
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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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