Usufulness of p16 and K-ras mutation in pancreatic adenocarcinoma and chronic pancreatitis differential diagnosis

• Autorzy: Talar-Wojnarowska R. , Gasiorowska A. , Smolarz B. , Romanowicz-Makowska H. , Strzelczyk J. , Janiak A. , Kulig A. , Malecka-Panas E.
• Języki publikacji: EN

Abstrakty

EN

The differentiation of chronic pancreatitis (CP) from pancreatic adenocarcinoma (PA) remains a great challenge. The purpose of the study was to compare the prevalence of p16 and K-ras mutation in PA and CP in order to evaluate their usefulness in differential diagnosis of those diseases. Methods: The study included 44 patients who underwent Whipple resection or distal pancreatectomy for PA (23 subjects) or CP (21 subjects). DNA from pancreatic tissue was analysed for K-ras mutation (codon 12) and p16 mutations with PCR amplifications. Results: The K-ras gene mutation has been shown in 17 (73,9%) cases with pancreatic adenocarcinoma which was significantly more often than in chronic pancreatitis - 9 (42,8%) (p<0,01). Prevalence of p16 mutations in patients with PA was 18 (78,3%) and with CP - 7 (33,3%) (p<0,01). K-ras and p16 mutations together have been observed in 16 (69,6%) cases in patients with PC and only in 3 (14,3%) - with CP (p<0,01). No statistically significant association between K-ras or p16 mutations and tumor size, sex or patient age has been observed. Conclusion: It is suggested that simultaneous measurement of K-ras and p16 mutations may provide an additional tool in differential diagnosis of chronic pancreatitis and pancreatic adenocarcinoma.

Słowa kluczowe

EN

stomach cancer; chronic pancreatitis; p16 mutation; colorectal cancer; K-ras mutation; pancreatic adenocarcinoma; gastrointestinal tract; diagnosis

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2004
• Tom: 55
• Numer: 2
• Opis fizyczny: p.129-138,fig.,ref.

Twórcy

autor

Talar-Wojnarowska R.

• Medical University, Kopcinskiego 22, 90-153 Lodz, Poland

autor

Gasiorowska A.

autor

Smolarz B.

autor

Romanowicz-Makowska H.

autor

Strzelczyk J.

autor

Janiak A.

autor

Kulig A.

autor

Malecka-Panas E.