Chromatographic separation of phenolic compounds from rapeseed by SE-HPLC - a short report

• Autorzy: Karamac M. , Kosinska A. , Rybarczyk A. , Pegg R.B.

Warianty tytułu

PL

Chromatograficzny rozdzial zwiazkow fenolowych rzepaku metoda SE-HPLC - krotki komunikat

• Języki publikacji: EN

Abstrakty

EN

Phenolic compounds from rapeseed were extracted into 80% (v/v) methanol. The crude extract was then separated using size exclusion high-performance liquid chromatography (SE-HPLC) on a TSK G2000SWXL column with a mobile phase of 45% acetonitrile and 0.1% TFA (v/v). Six peaks were identified on the chromatogram: the main peak originated from sinapin. Calibration of the assay was characterised by an excellent linearity of the sinapin standard (r2=1.0). The method so described can be used for screening phenolic constituents in rapeseed or canola. It is also useful for the purification of the sinapin as a standard from the crude extract.

PL

Związki fenolowe zawarte w nasionach rzepaku ekstrahowano 80% metanolem. Uzyskany ekstrakt poddano analizie metodą SE-HPLC na kolumnie TSK G2000SWXL. Fazą ruchomą był 45% acetonitryl (v/v) z dodatkiem 0.1% TFA. Na chromatogramie zanotowano obecność sześciu pików pochodzących od fenolokwasów i ich pochodnych. Głównym związkiem była sinapina. Kalibracja metody charakteryzowała się doskonałą liniowością wzorca sinapiny (r2 = 1). Opisana metoda może znaleźć zastosowanie w analizie przesiewowej składników fenolowych nasion rzepaku lub kanoli oraz przy oczyszczaniu standardu sinapiny z surowego ekstraktu.

Słowa kluczowe

EN

chromatographic separation; phenolic compound; separation; high performance liquid chromatography; phenolic acid; rapeseed

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2007
• Tom: 57
• Numer: 1
• Opis fizyczny: p.87-90,fig.,ref.

Twórcy

autor

Karamac M.

• Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland

autor

Kosinska A.

autor

Rybarczyk A.

autor

Pegg R.B.

Bibliografia

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