EN
The tissue culture of Ch. cinerariaefolium was maintained on the different media. Addition of carbendazim (500 mg/dm3) and ascorbinic acid (1000 mg/dm3) strongly stimulated Pyrethrins biosynthesis. Differentiation of calluss culture on medium with gibberelic acid GA3 remarkably increased yield of Pyrethrins. The cell suspension culture was initiated from callus. Addition of Pyrethrins biosynthesis stimulators to cell suspensions had similar effect as in callus cultures, but Pyrethrins content in suspension was significantly lower. Amounts of Pyrethrins in both callus and suspension cultures however were considerably (about 10 times) lower than in parent plants.