Protective effect of pCI-neo vector-mediated DNA vaccine against Chlamydophila abortus in mice

• Autorzy: Li S , He C. , Ling Y. , Wang H. , Zhao J. , Yao B.
• Języki publikacji: EN

Abstrakty

EN

In the presented study, omp-1 gene coding main outer membrane protein of Chlamydophila abortus was cloned into pCI-neo and pcDNA3.1 as delivery vehicles for DNA vaccination. Thirty-six BALB/c mice were randomly assigned to three groups and inoculated intramuscularly with: 1) 100 µg of pCI-neo, 2) 100 µg of pCI-neo::MOMP, and 3) 100 µg of pcDNA3.1::MOMP. All animals were vaccinated three times at 14 d intervals. The results showed that mice given pCI-neo::MOMP developed a higher IgG antibody level, high T lymphocytes proliferations, and high titres of IFN-γ and IL-2, than mice given pcDNA3.1::MOMP, which induced moderate antibody levels, less T lymphocyte proliferations and lower cytokine levels. No significant difference of TNF-α was observed in above groups. Additionally, IgG2a and Ig2b were the predominant isotypes on day 44, suggesting a high level of Th1 stimulation. Mice given the pCI-neo::MOMP also elicited a higher chlamydial clearance and a better protection than mice with pcDNA3.1::MOMP did. Immunisation with pCI-neo::MOMP vaccine may provide novel ways for active immunisation strategy against Chlamydophila abortus.

Słowa kluczowe

EN

pCI-neo vector; pcDNA3.1 vector; DNA vaccination; mouse; mice; Chlamydophila abortus

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2008
• Tom: 52
• Numer: 2
• Opis fizyczny: p.217-222,fig.,ref.

Twórcy

autor

Li S

• Huazhong Agricultural University, Wuhan 430070, China

autor

He C.

autor

Ling Y.

autor

Wang H.

autor

Zhao J.

autor

Yao B.

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