Expression of a constitutively active mutant of heat shock factor 1 under the control of testis-specific hst70 gene promoter in transgenic mice induces degeneration of seminiferous epithelium

• Autorzy: Widlak W. , Benedyk K. , Vydra N. , Glowala M. , Scieglinska D. , Malusecka E. , Nakai A. , Krawczyk Z.
• Języki publikacji: EN

Abstrakty

EN

Heat shock activates in somatic cells a set of genes encoding heat shock proteins which function as molecular chaperones. The basic mechanism by which these genes are activated is the interaction of the specific transcription factor HSF1 with a regula­tory DNA sequence called heat shock element (HSE). In higher eukaryotes HSF1 is present in unstressed cells as inactive monomers which, in response to cellular stress, aggregate into transcriptionally competent homotrimers. In the present paper we showed that the expression of a transgene encoding mutated constitutively active HSF1 placed under the control of a spermatocyte-specific promoter derived from the hst70 gene severely affects spermatogenesis. We found the testes of transgenic mice to be significantly smaller than those of wild-type males and histological analysis showed massive degeneration of the seminiferous epithelium. The lumen of tubules was devoid of spermatids and spermatozoa and using the TUNEL method we demonstrated a high rate of spermatocyte apoptosis. The molecular mechanism by which constitutively active HSF1 arrests spermatogenesis is not known so far. One can assume that HSF1 can either induce or repress so far unknown target genes involved in germ cell apoptosis.

Słowa kluczowe

EN

hst70 gene; mouse; mice degeneration; heat shock; gene encoding; seminiferous epithelium; transgenic animal; somatic cell; apoptosis; spermatogenesis

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 2
• Opis fizyczny: p.535-541,fig.

Twórcy

autor

Widlak W.

• Center of Oncology, Maria Sklodowska-Curie Memorial Institute, 44-100 Gliwice, Poland

autor

Benedyk K.

autor

Vydra N.

autor

Glowala M.

autor

Scieglinska D.

autor

Malusecka E.

autor

Nakai A.

autor

Krawczyk Z.

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