Embryological studies on ovules of Melandrium album pollinated in vitro with Lychnis coronaria pollen grains

• Autorzy: Zenkteler M , Bagniewska-Zadworna A. , Zenkteler E.
• Języki publikacji: EN

Abstrakty

EN

Placentae of Melandrium album excised from ovaries of unopened flowers were pollinated in vitro with pollen grains of Lychnis coronaria. Pollen grains germinated, and pollen tubes were found entering the micropyles 18 h after pollination. Double fertilization occurred. Immature and mature hybrid embryos at different stages of development were analyzed up to the third week of culture. Twenty-five days after culture, cotyledonary embryos were isolated from ovules and transferred to MS medium supplemented with 0.5, 1 or 2 mg l-1 indole-3-acetic acid (IAA). About 85% of the 115 inoculated embryos died shortly after transfer to medium, due to atrophy of the root and one cotyledon. Eighteen embryos survived; of these, 16 well-formed seedlings with root system were transferred to soil. The hybrid plants grew normally, and their hybridity was confirmed by morphological study. Female and male organs of the intergeneric hybrid were completely sterile.

Słowa kluczowe

EN

intergeneric cross; development; Lychnis coronaria; Melandrium album; botany; pollination; hybrid embryo; in vitro

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2005
• Tom: 47
• Numer: 1
• Opis fizyczny: p.135-138,fig.,ref.

Twórcy

autor

Zenkteler M

• Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland

autor

Bagniewska-Zadworna A.

autor

Zenkteler E.

Bibliografia

• Murashige T, and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 15: 473-497.
• Prentice HC. 1978. Experimental taxonomy of Silene section Elisanthe (Caryophyllaceae) crossing experiments. Botanical Journal of Linnean Society 77: 203-216.
• Zenkteler M. 1967. Test-tube fertilization of ovules of Melandrium album Mill. with pollen grains of several species of the Caryophyllaceae family. Experientia 23: 775-777.
• Zenkteler M. 1969. Test-tube fertilization and development of hybrids between some species of the Caryophyllaceae family. PTPN Pr Kom Biol 32: 1-71.
• Zenkteler M. 1980. Intraovarian and in vitro pollination. International Review of Cytology, suppl 11B: 137-156.
• Zenkteler M. 1999. In vitro pollination of excised ovaries. Acta Biologica Cracoviensia Series Botanica 41: 31-38.
• Zenkteler M, Misiura E, and Guzowska I. 1975. Studies on obtaining hybrid embryos in test tubes. In: Mohan Ram HY, Shah JJ, and Shah CK [eds.], Form, structure and function in plants: 180-187. Sarita Prakashan, Merrut, India.
• Zluvova J, Lengerova M, Markova M, Hobza R, Nicolas M, Vyskot B, CharlesworthD, Negrutiu I, and Janousek B. 2005. The interspecific hybrid Silene latifolia x S. viscosa reveals early events of sex chromosome evolution. Evolution and Development 7: 327-336.