Yeast nuclear PET127 gene can suppress deletions of the SUV3 or DSS1 genes: An indication of a functional interaction between 3' and 5' ends of mitochondrial mRNAs

• Autorzy: Wegierski T. , Dmochowska A. , Jablonowska A. , Dziembowski A. , Bartnik E. , Stepien P.P.
• Języki publikacji: EN

Abstrakty

EN

Saccharomyces cerevisiae nuclear genes SUV3 and DSS1 encode putative RNA helicase and RNase II, respectively, which are subunits of the mitochondrial degradosome (mtEXO): a three-protein complex which has a 3' to 5' exoribonuclease activity and plays a major role in regulating stability of mitochondrial RNA. Lack of either of the two gene products results in a respiratory negative phenotype, while on the molecular level it causes a total block of mitochondrial translation, loss of the in vitro exoribonuclease activity and changes in stability and processing of many mtRNAs. We have found that the yeast nuclear gene PET127 present on a low or high copy number vector can effectively suppress the effects of the SUV3 or DSS1 gene disruptions. Since the product of the PET127 gene is involved in processing of the 5' ends of mitochondrial mRNAs, we suggest that there is a functional coupling between the 5' and 3' ends of mitochondrial mRNAs.

Słowa kluczowe

EN

yeast; PET127 gene; mitochondrion; SUV3 gene; protein complex; DSS1 gene; Saccharomyces cerevisiae

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 4
• Opis fizyczny: p.935-940,fig.

Twórcy

autor

Wegierski T.

• University of Warsaw, Warsaw, Poland

autor

Dmochowska A.

autor

Jablonowska A.

autor

Dziembowski A.

autor

Bartnik E.

autor

Stepien P.P.

Bibliografia

• 1. Dziembowski, A., Malewicz, M., Mińczuk, M., Golik, P., Dmochowska, A. & Stepień, P.P. (1998) The yeast nuclear gene DSSl coding for a putative RNase II is necessary for the functioning of the mitochondrial degrado- some in processing and turnover of RNA. Mo- lec. Gen. Genet. 260, 108-114.
• 2. Margossian, S.P., Li, H., Zassenhaus, H.P. & Butów, R.A. (1996) The DExH box protein Suv3p is a component of a yeast mitochon­drial 3'- to 5'-exoribonuclease that suppresses group I intron toxicity. Cell 84, 199-209.
• 3. Stepien, P.P., Margossian, S.P., Landsman, D. & Butow, R.A. (1992) The yeast nuclear gene suv3 affecting mitochondrial postrtrans- criptional processes encodes a putative ATP- dependent RNA helicase. Proc. Natl. Acad. Sei. U.S.A. 89, 6813-6817.
• 4. Dmochowska, A., Golik, P. & Stępień, P.P. (1995) The novel nuclear gene DSS-1 of Sac- charomyces cerevisiae is necessary for mito­chondrial biogenesis. Curr. Genet. 28, 108- 112.
• 5. Golik, P., Szczepanek, T., Bartnik, E., Stępień, P.P. & Łazowska, J. i 1995) The S. cerevisiae nuclear gene SUV3 encoding a putative RNA helicase is necessary for the stability of mito­chondrial transcripts containing multiple in- trons. Curr. Genet 28, 217-224.
• 6. Min, J.J. & Zassenhaus, H.P. (1993) Identifi­cation of a protein complex that binds to a do-decamer sequence found at the 3' ends of yeast mitochondrial mRNAs. Mol Cell Biol 13, 4167-4173.
• 7. Staples, R.R. & Dieckmann, C.L. (1993) Gen­eration of temperature-sensitive cbpl strains of Saccharomyces cerevisiae by PCR mutagene­sis and in vivo recombination: Characteristics of the mutant strains imply that CBP1 is in­volved in stabilization and processing of cyto chrome b pre-mRNA. Genetics 135,981-991.
• 8. Staples, R.R. & Dieckmann, C.L. (1994) Sup­pressor analysis of temperature-sensitive cbpl strains of Saccharomyces cerevisiae: The prod­uct of the nuclear gene SOCl affects mito­chondrial cytochrome b mRNA posttranscrip- tionally. Genetics 138, 565-575.
• 9. Wiesenberger, G. & Fox, T.D. (1997) Pet 127, a membrane-associated protein involved in sta­bility and processing of Saccharomyces cerevi­siae mitochondrial RNAs. Mol Cell, Biol 17, 2816-2824.
• l0.Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning. A laboratory man­ual. Cold Spring Harbor Laboratory Press.
• 11. Stępień, P.P., Kokot, L., Łęski, T. & Bartnik, E. (1995) The suv3 nuclear gene product is re­quired for the in vivo processing of the yeast mitochondrial 2IS rRNA transcripts contain­ing the rl intron. Curr. Genet. 27, 234-238.
• 12. Tarun, S.Z. & Sachs, A.B. (1996) Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G. EMBO J. 15, 7168-7177.
• 13. Tarun, S.Z., Jr. & Sachs, A.B. (1995) A com­mon function for mRNA 5' and 3' ends in translation initiation in yeast. Genes Dev. 9, 2997-3007.
• 14. Wells, S.E., Hill ner, P.E., Vale, R.D. & Sachs, A.B. (1998) Circularization of mRNA by eukaryotic translation initiation factors. Mol. Cell 2, 135-140.