Nowy wariant genu amelogeniny u bydla

• Autorzy: Grzybowski G , Prusak B. , Trela J.

Warianty tytułu

EN

New variant of the amelogenin gene in cattle

• Języki publikacji: PL

Abstrakty

EN

The aim of this work was to search for a sequence polymorphism in the amelogenin gene (AMEL-X) in the region of exon 6. The material covered 653 cows and bulls of variable breeds, typical for their karyotype. The cows used in this study belong to Polish Red (150), Polish Whiteback (72), Red-and-White (72), Black-and-White with Holstein-Friesian blood share (69). The male material used in this study covered 269 sires (Black-and-White with Holstein-Friesian blood share - 125, Polish Red - 12, pure breed Holstein-Friesian - 67, Red-and-White - 30, Simmental - 27 and different meat breeds - 7). Six young bulls of Polish Whiteback and 15 young bulls of Polish Red were also tested. The polymerase chain reaction (PCR) primers used in this study were given by Ennis and Gallagher. The PCR products were separated on ABI PRISM 377 DNA sequencer (AB). For the AMEL-X gene copy the specific PCR product of 278bp was amplified and for the AMEL-Y gene copy the specific PCR product was 215bp long. This typical pattern was observed in cows and bulls of all tested cattle breeds except for the Polish Red. In addition to the typical signal for the chromosome X in 24 cows and 2 bulls of Polish Red a signal for the PCR product of 269bp was detected. This result shows that in an amplified region of AMEL gene in copy on chromosome X the sequence polymorphism is present and its molecular origin is a deletion of 9bp.

Słowa kluczowe

PL

bydlo; gen amelogeniny; genetyka zwierzat

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 06
• Opis fizyczny: s.692-695,rys.,tab.,bibliogr.

Twórcy

autor

Grzybowski G

• Instytut Genetyki i Hodowli Zwierzat PAN w Jastrzebcu, ul.Postepu 1, 05-552 Wolka Kosowska

autor

Prusak B.

autor

Trela J.

Bibliografia

• 1. Aoba T.: Recent observations on enamel crystal formation during mammalian amelogenesis. Anat. Rec. 1996, 245, 208-218.
• 2. Delgado S., Casane D., Bonnaud L., Laurin M., Sire J.-Y., Girondot M.: Molecular evidence for Precambrian origin of amelogenin, the major protein of vertebrate enamel. Mol. Biol. Evol. 2001, 18, 2146-2153.
• 3. Delgado S., Girondot M., Sire J.-Y.: Molecular evolution of amelogenin in mammals. J. Mol. Evol. 2005, 60, 12-30.
• 4. Deutsch D.: Structure and function of enamel gene products. Anat. Rec. 1989, 224, 189-210.
• 5. Ennis S., Galagher T. F.: A PCR-based sex determinartion assay in cattle based on the bovine amelogenin locus. Anim. Genet. 1994, 25, 425-427.
• 6. Gibson C., Golub E., Herold R., Risser M., Ding W., Shimokawa H., Young M., Termine J., Rosenbloom J.: Structure and expression of the bovine amelogenin gene. Biochemistry 1991, 30, 1075-1079.
• 7. Gibson C. W., Golub E. E., Abrams W. R., Shen G., Ding W., Rosenbloom J.: Bovine amelogenin message heterogeneity: Alternative splicing and Y-chromosome gene transcription. Biochemistry 1992, 31, 8384-8388.
• 8. Grzybowski G, Dymnicki E.: Metody oraz ekonomiczne znaczenie sterowania proporcją płci u bydła. Biotechnologia 1997, 3, 38-50.
• 9. Grzybowski G., Prusak B.: Genetic variation in nine European cattle breeds as determined on the basis of microsatellite markers. III. Genetic integrity of the Polish Red cattle included in the breeds preservation programme. Anim. Sci. Pap. Rep. 2004, 22, 45-56.
• 10. Hart P. S., Hart T. C., Simmer J. P., Wright J. T.: A nomenclature for X-linked amelogenesis imperfecta. Arch. Oral Biol. 2002, 47, 255-260.
• 11. Lechniak D., Cumming I. R.: The use of amelogenin gene polymorphism in PCR embryo sexing in bovine IVF embryos. J. Appl. Genet. 1997, 38, 45-49.
• 12. Lubieniecka J., Grzybowski G., Lubieniecki K.: Genetic variation in nine European cattle breeds as determined on the basis of microsatellite markers I. Within-breed variation. Anim. Sci. Pap. Rep. 2001, 19, 249-264.
• 13. Miścicka-Śliwka D., Grzybowski T., Woźniak M.: Optimization of a hexaplex DNA amplification from short tandem repeat and amelogenin loci. Electrophoresis 1997, 18, 1627-1632.
• 14. Reklewski Z.: Hodowla zachowawcza bydła rasy polskiej czerwonej. Wiadom. Zoot. 2005, XLIII, 98-101.
• 15. Reklewski T., Grzybowski G., Lubieniecki K.: Sex identification in cattle on the basis of amelogenin polymorphism. Živočišna Výroba 1996, 41, 504-505.
• 16. Sire J. Y., Delgado S., Fromentin D., Girondot M.: Amelogenin: lessons from evolution. Arch. Oral Biol. 2005, 50, 205-212.
• 17. Szarek J., Adamczyk K.: Zarys historyczny bydła polskiego czerwonego. Wiadom. Zoot. 2005, XLIII, 3-12.
• 18. Toyosava S., O'Huigin C., Figueroa F., Tichy H., Klein J.: Identification and characterization of amelogenin genes in monotremes, reptiles, and amphibians. Proc. Natl. Acad. Sci. USA 1998, 95, 13056-13061.
• 19. Veis A.: Amelogenin gene splice products: potential signaling molecules. Cell. Mol. Life Sci. 2003, 60, 38-55.