Wystepowanie oraz lekowrazliwosc opornych na karbapenemy szczepow Pseudomonas aeruginosa izolowanych z materialu klinicznego w latach 1998-2009

• Autorzy: Bogiel T. , Mikucka A. , Skalski T. , Gospodarek E.

Warianty tytułu

EN

Occurrence and susceptibility to antibiotics of carbapenem-resistant Pseudomonas aeruginosa strains between 1998 and 2009

• Języki publikacji: PL

Abstrakty

PL

Przeprowadzono retrospektywną analizę częstości izolacji oraz lekowrażli- wości 775 szczepów Pseudomonas aeruginosa opornych na imipenem i me- ropenem. Szczepy objęte badaniem, pochodzące z materiału od 568 chorych, izolowano w latach 1998-2009 w Zakładzie Mikrobiologii Klinicznej Szpitala Uniwersyteckiego nr 1 im. dr. A. Jurasza w Bydgoszczy. W okresie objętym badaniem odnotowano tendencję wzrostową częstości izolacji szczepów P. aeruginosa o fenotypie oporności na karbapenemy.

EN

P. aeruginosa rods are dangerous pathogens mainly responsible for nosocomial infections of different localization. Resistance to carbapenems, observed among them, is a serious threat due to ability to be transmitted between bacterial species. The aim of our study was to retrospectively evaluate the frequency of isolation and susceptibility to antibiotics of imipenem- and meropenem-resistant P. aeruginosa strains isolated between 1998 and 2009 from patients of University Hospital No 1 of dr A. Jurasz in Bydgoszcz. Study shows increasing number of isolation that type of strains from 19 in 1998 to 144 in 2009. Among all isolated P. aeruginosa strains majority was obtained from patients of the Intensive Care Units, Rehabilitation and Surgery Clinics. Examined strains of P. aeruginosa rods were mainly isolated from urine (20,5%), bronchoalveolar lavage (17,7%) and wound swabs (14,5%) samples. The isolates demonstrated frequently resistance to carbenicillin (≥66,7%), ticarcil- lin (≥72,7%) and cefotaxime (≥75,6%). The lowest rate of resistant strains was observed in case of ceftazidime (≤68,8%), aztreonam (≤47,4%) and Colistin (≤1,7%) suggesting the highest activity of that antimicrobials against infections caused by examined strains.

Słowa kluczowe

PL

szczepy bakteryjne; Pseudomonas aeruginosa; patogeny; zakazenia szpitalne; wrazliwosc na leki; antybiotyki karbapenemowe; opornosc na antybiotyki; material kliniczny

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2010
• Tom: 62
• Numer: 3
• Opis fizyczny: s.221-229,rys.,tab.,bibliogr.

Twórcy

autor

Bogiel T.

• Collegium Medicum w Bydgoszczy, Uniwersytet Mikolaja Kopernika w Toruniu, ul.M.Sklodowskiej-Curie 9, 85-094 Bydgoszcz

autor

Mikucka A.

autor

Skalski T.

autor

Gospodarek E.

Bibliografia

• 1. Bogiel T, Deptuła A, Gospodarek E. Ocena aktywności doripenemu wobec pałeczek Pseudomonas sp. i Acinetobacter sp. Med Dośw Mikrobiol 2009; 61: 367-74.
• 2. Clinical and Laboratory Standards Institute. Perfomance standards for antimicrobial susceptibility testing. Eighteenth informational supplement (M100-S18). CLSI Wayne, Pa 2008.
• 3. El Amin N, Giske CG, Jalal S i inni. Carbapenem resistance mechanisms in Pseudomonas aeruginosa'. alterations of porin OprD and efflux proteins do not fully explain resistance patterns observed in clinical isolates. APMIS 2005; 113: 187-96.
• 4. Fiett J, Baraniak A, Mrówka A i inni. Molecular epidemiology of acquired-Metallo-ß-lactamase- producing bacteria in Poland. J Antimicrob Chemother 2006; 50: 880-6.
• 5. Fujimura T, Anan N, Sugimori G i inni. Susceptibility of Pseudomonas aeruginosa clinical isolates in Japan to doripenem and other antipseudomonal agents. Int J Antimicrob Agents 2009; 34: 523-8.
• 6. Furtado GH, Bergamasco MD, Menezes FG i inni. Imipenem-resistant Pseudomonas aeruginosa infection at a medical-surgical intensive care unit: risk factors and mortality. J Crit Care 2009; 24: 9-14.
• 7. Gniadkowski M, Żabicka D, Hryniewicz W. Rekomendacje doboru testów do oznaczania wrażliwości bakterii na antybiotyki i chemioterapeutyki 2009. Oznaczanie wrażliwości pałeczek Gram-ujemnych.
• 8. Gupta V. Metallo-beta-lactamases in Pseudomonas aeruginosa and Acinetobacter species. Expert Opin Investig Drugs 2008; 17: 131-43.
• 9. Hammami S, Ghozzi R, Burghoffer В i inni. Mechanisms of carbapenem resistance in non-metallo-beta-lactamase-producing clinical isolates of Pseudomonas aeruginosa from a Tunisian hospital. Pathol Biol (Paris) 2009; 57: 530-5.
• 10. Harris AD, Smith D, Johnson JA i inni. Risk factors for imipenem-resistant Pseudomonas aeruginosa among hospitalized patients. Clin Infect Dis 2002; 34: 340-5.
• 11. Joly-Guillou ML, Kempf M, Cavallo JD i inni. Comparative in vitro activity of meropenem, imipenem and piperacillin/tazobactam against 1071 clinical isolates using 2 different methods: a French multicentre study. BMC Infect Dis 2010; 18: 72.
• 12. Kohlenberg A, Weitzel-Kage D, van der Linden P i inni. Outbreak of carbapenem-resistant Pseudomonas aeruginosa infection in a surgical intensive care unit. J Hosp Infect 2010; 74: 350-7.
• 13. Kucisec-Tepes N. Pseudomonas aeruginosa-а significant hospital pathogen and resistance to carbapenem. Acta Med Croatica 2004; 58: 313-21.
• 14. Lautenbach E, Synnestvedt M, Weiner MG i inni. Imipenem resistance in Pseudomonas aeruginosaі: emergence, epidemiology, and impact on clinical and economic outcomes. Infect Control Hosp Epidemiol 2010; 31: 47-53.
• 15. Maniati M, Ikonomidis A, Mantzana P i inni. A highly carbapenem-resistant Pseudomonas aeruginosa isolate with a novel blaVIM-4/blaP1B integron overexpresses two efflux pumps and lacks OprD. J Antimicrob Chemother 2007; 60: 132-5.
• 16. Młynarczyk G, Sawicka-Grzelak A, Szymanek K i inni. Resistance to carbapenems among Pseudomonas aeruginosa isolated from patients of transplant wards. Transplant Proc 2009; 41: 3258-60.
• 17. Kikuchi T, Nagashima G, Taguchi K i inni. Contaminated oral intubation equipment associated with an outbreak of carbapenem-resistant Pseudomonas in an intensive care unit. J Hosp Infect 2007; 65: 54-7.
• 18. Onguru P, Erbay A, Bodur H i inni. Imipenem-resistant Pseudomonas aeruginosa: risk factors for nosocomial infections. J Korean Med Sci 2008; 23: 982-7.
• 19. Pena C, Dominguez MA, Pujol M i inni. An outbreak of carbapenem-resistant Pseudomonas aeruginosa in a urology ward. Clin Microbiol Infect 2003; 9: 938-43.
• 20. Sacha P, Wieczorek P, Jakoniuk P i inni. Participation of metallo-beta-lactamases in resistance to imipenem and meropenem in clinical strains of Pseudomonas aeruginosa and Acinetobacter spp. Wiad Lek 2008; 61: 30-6.
• 21. Suárez C, Peña C, Gavaldà L i inni. Influence of carbapenem resistance on mortality and the dynamics of mortality in Pseudomonas aeruginosa bloodstream infection. Int J Infect Dis 2010; 9.