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1995 | 36 | 3 |

Tytuł artykułu

Genetic polymorphism and quantitative variation of alpha-amylases from rye endosperm

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
α-Amylase isozymes from rye endosperm were analysed by means of isoelectric focusing, polyacrylamide gel electrophoresis, immunoelectrophoresis and colorimetric assay. Α-AMY 1 (high pI) group was separated into 13 IEF bands, whereas in group α-AMY2 (low pI) 2 intensive and 6-8 faint bands were found. Two linked (2±1 cM) polymorphic loci and a single locus with two alleles encoding for α-AMY 1 and α-AMY 2 groups, respectively, were identified after genetic analysis of the IEF patterns. All α-amylase isozymes developed on PAGE, were shown to belong to α-AMY 1 group. It was demonstrated that a single PAGE isozyme corresponds to 2-4 separate IEF bands and that most of the IEF bands can be attributed to more than one PAGE isozyme. The activity of α-amylases from PAGE zone I was 2.3 times higher than the activity of zone II isozymes. A strong correlation between the activity and protein amount of particular α-AMY 1 isozymes (r=0.94) was found.

Wydawca

-

Rocznik

Tom

36

Numer

3

Opis fizyczny

p.197-204,fig.

Twórcy

autor
  • Agricultural University, Slowackiego 17, 71-434 Szczecin, Poland
autor

Bibliografia

  • AINSWORTH C.C., MILLER T.E., GALE M.D. (1987). α-Amylase and ß-amylasc homoeoloci in spccics related to wheat. Genet. Res., Camb. 49: 93-103.
  • ALLARD R.W. (1956). Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24: 235-278.
  • BARNES W.C., BLAKENEY A.B. (1974). Determination of cereal alpha-amylase using a commercially available dye-labelled substrate. Die Starke 26: 193-197.
  • BAULCOMBE D.C., HUTTLY A.K., MARTIENSEN R.A., BARKER R.F., JARVIS M.G. (1987). A novel wheat α-amylase gene (α-Amy3). Mol. Gen. Genet. 209: 33-40.
  • CLARKE H.G.M., FREEMAN T. (1967). A quantitative immunoelectrophoresis method (H. Peeters ed.). Prot. Biol. Fluids 14, Elsevier, Amsterdam, pp. 503-509.
  • DAUSSANT J., RENARD H.A. (1987). Development of different α-amylase isozymes, having high and low isoelectric points during early stages of kernel development in wheat. J. Cereal Sci. 5: 13-21.
  • GALE M.D., LAW C.N., CHOJECKI A.J., KEMPTON R.A. (1983). Genetic control of α-amylase production in wheat. Theor. Appl. Genet. 64: 309-316.
  • KHOREVA V.L., KUDRYAKOVA N.N., KOBYLYANSKY V.D., LAPIKOV N.S. (1985). Genotypical variability of α-amylase in rye clones with various resistance to seed germination in ear. Agrie. Biol. (Russ.) 2: 43-45.
  • LAZARUS C.M., BAULCOMBE D.C., MARTIENSSEN R.A. (1985). α-Amylase genes of wheat arc two multigene families which are differentially expressed. Plant Mol. Biol. 5: 13-24.
  • LAURELL C.B. 1966. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal. Biochem. 15: 45-52.
  • ŁAPIŃSKI M. MASOJĆ P. (1983). Polymorphism of amylases from rye endosperm. 1. Variability of the electrophoretic patterns of amylases from inbred lines. Genet. Pol. 24: 131-137.
  • MacGREGOR A.W. (1980). Action of malt α-amylases on barley starch granules. MBAA Technical Quaterly 17: 215-221.
  • MacGREGOR A.W., BALLANCE D. (1980). Quantitative determination of α-amylase enzymes in germinated barley after separation by isoelectric focusing. J. Inst. Brew., London 86: 131-133.
  • MacGREGOR A.W., MARCHYLO B.A., KRUGER J.E. (1988). Multiple α-amylase components in germinated cereal grains determined by isoelectric focusing and chromatofocusing. Cereal Chem. 65: 326-333.
  • MacGREGOR A.W., MORGAN J.E. (1992). Determination of specific activities of malt α-amylases. J. Cereal Sci. 16: 267-277.
  • MACKO V., HONOLD G.R., STAHMANN M.A. (1967). Soluble proteins and multiple enzyme forms in early growth of wheat. Phytochem. 6: 465-471.
  • MASOJĆ P. (1987). Genetics of α-amylases from rye endosperm. Theor. Appl. Genet. 73: 440-444.
  • MASOJĆ P., GALE M.D. (1990). The factor modifying α-amylase isozyme pattern from rye endosperm is an endogenous α-amylase inhibitor. Hcreditas 113: 151-155.
  • MASOJĆ P., GALE M.D. (1991). α-Amylase structural genes in rye. Theor. Appl. Genet. 82: 771-776.
  • NISHIKAWA K., FURUTA Y., HINA Y., YAMADA T. (1981). Genetic studies of α-amylase isozymes in wheat. 4. Genetic analyses in hexaploid wheat. Jpn. J. Genet. 56: 385-395.
  • ROZHKOV Y.I. (1983). Electrophoretic division of multiple enzyme forms in substrate- containing media. Agrie. Biol. (Russ.) 1: 107-115.
  • SHARP P.J., KREIS M., SHEWRY P.R., GALE M.D. (1988). Location of ß-amylase sequences in wheat and its relatives. Theor. Appl. Genet. 75: 286-290.
  • WAGENAAR S., LUGTENBORG T.F. (1973). α-Isoamylases of rye seeds. Phytochem. 12: 1243-1247.

Typ dokumentu

Bibliografia

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