Poly(ADP-ribose) polymerase-1 is a novel nuclear target for cholinergic receptor signaling in the hippocampus

• Autorzy: Strosznajder R.P. , Jesko H. , Adamczyk A.
• Języki publikacji: EN

Abstrakty

EN

Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear enzyme involved in DNA repair and transcription regulation. The aim of this study was to investigate the role of PARP-1 in muscarinic cholinergic receptor signaling. Our data indicate that activation of muscarinic cholinergic receptors by carbachol (1mM) in the presence of GTPS evoked a significant enhancement of PARP activity in the adult rat hippocampus. Moreover, TMB-8 (10µM), an antagonist of inositol 1, 4, 5 trisphosphate (IP3) receptor prevented the activation of PARP-1, which indicates that IP3 /Ca2+ signaling is involved in this pathway. The diacylglycerol (DAG)-regulated protein kinase C (PKC) inhibitor (GF109203X) (1µM) only slightly enhanced PARP activity in hippocampal nuclear fractions, which suggests that DAG/ PKC is not involved in PARP activation.

Słowa kluczowe

EN

protein kinase C; muscarinic receptor signalling; poly[ADP-ribose] polymerase-1; DNA repair; hippocampus; cholinergic receptor

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2005
• Tom: 56
• Numer: 4
• Opis fizyczny: p.209-213,fig.,ref.

Twórcy

autor

Strosznajder R.P.

• Polish Academy of Sciences, 5 Pawinskiego St., 02-106 Warsaw, Poland

autor

Jesko H.

autor

Adamczyk A.