Detection of group a rotaviruses in field samples from diarrheic piglets using cDNA probes

• Autorzy: Winiarczyk S
• Języki publikacji: EN

Abstrakty

EN

In this study DNA probes complementary to sequence of bovine and porcine rotavirus genes 6 (Rc6), 11 (Rc611) and 9 (Rc549), respectively, were developed and examined to determine their ability to detect group A porcine rotaviruses. In the dot biot hybridization test, the heterologous probes - Rc6 and Rc611 - detected 20 ng dsRNA of reference strains of porcine rotaviruses OSU and Gottfried whereas the homologous probe Rc549 detected as little as 1 ng. Out of 117 RNA extracted of feces of diseased piglets, rotavirus RNA was found in 23 cases using Rc6 and Rc549 probes. In this study, the homologous Rc549 probe was found to have the best diagnostic utility.

Słowa kluczowe

EN

cDNA probe; rotavirus; veterinary medicine; porcine rotavirus; piglet; diarrhea; detection; DNA probe

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2001
• Tom: 45
• Numer: 1
• Opis fizyczny: p.29-35,fig.

Twórcy

autor

Winiarczyk S

• University of Agriculture, 20-612 Lublin, Poland

Bibliografia

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