Rapid in vitro propagation of Eclipta alba [L.] Hassk. through high frequency axillary shoot proliferation

• Autorzy: Husain M K , Anis M.
• Języki publikacji: EN

Abstrakty

EN

An efficient protocol has been developed for rapid in vitro propagation of Eclipta alba L. (Asteraceae) through axillary bud multiplication. Murashige and Skoog (MS) medium supplemented with BA (10 pM) was found to be most effective in breaking bud dormancy. An average number of 23 ± 0.57 shoots per explant was recorded after 30 days. Culture of node segments on fresh medium with lower concentration of BA (2 pM) enhanced the multiplication rate. A maximum of 79 ± 1.90 mean number of shoots were obtained after three subcultures without any decline in multiplication rate. The regenerated microshoots showed the most efficient rooting on half strength MS medium augmented with 0.5 pM IBA. Plantlets went through a hardening phase prior to ex vitro transfer and established in earthen pots containing garden soil; survival of about 90 %. The established plants were uniform and exhibited morphological characters identical to mother plants.

Słowa kluczowe

EN

Compositae; Eclipta alba; herb; plant physiology; micropropagation; greenhouse evaluation; medicinal plant; in vitro

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2006
• Tom: 28
• Numer: 4
• Opis fizyczny: p.325-330,fig.,ref.

Twórcy

autor

Husain M K

• Aligarh Muslim University, Aligarh 202 002, India

autor

Anis M.

Bibliografia

• Anonymous 2003. The wealthofIndia: Raw Materials, Vol 3, CSIR Publication: New Delhi, 127-128.
• Anonymous 1998. Opportunities and problems for the production and development of medicinal plants in India. Industry Highlights, Central Drug Research Institute, Lucknow, 21: 29-34.
• Borthakur M, Dutta K, Nath SC, Singh RS. 2000. Micropropagation of Eclipta alba and Eupatorium adenophorum using a single-step nodal cutting techt nique. Plant Cell Tiss. Org. Cult. 62: 239-242.
• Casado JP, Navarro MC, Utrilla MP,Martinez A, Jimenez J. 2002 Micropropagation of Santolina canescens Lagasca and in vitro volatiles production by shoot explants. Plant Cell Tiss. Org. Cult. 69: 147-153.
• Dias MC, Almeida R, Romano A. 2002. Rapid clonal multiplication of Lavandula viridis L Her through in vitro axillary shoot proliferation. Plant Cell Tiss. Organ Cult. 68: 99-102.
• Faisal M , Anis M. 2003 Rapid mass propagation of Tylophora indica Merill via leaf callus culture. Plant Cell Tiss. Org. Cult. 75: 125-129.
• Faisal M, Ahmad N, Anis M. 2005. Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tiss. Org. Cult. 80: 187-190.
• Franca SC, Bertani BW, Pereira AMS. 1995. Anti- hepatotoxic agent in micropropagated plantlets of Eclipta alba. Plant Cell Tiss. Org. Culture 40: 297-299.
• Fraternale D, Giamperi L, Ricci D, Rocchi MBL. 2002. Micropropagation of Bupleurum fruticosum: the effect of tricontanol. Plant Cell Tiss. Organ Cult. 69: 135-140.
• Komalavalli N, Rao MV. 2000. In vitro micropro t pagation of Gymnema sylvestre- A multipurpose medicinal plant. Plant Cell Tiss. Organ Cult. 61: 97-105.
• Mors WB, Nascimento MC, Parente JP, Silva MH, Melo PA, Saurez- Kurtz G. 1989. Neutralization of lethal and myotoxic activities of South American rattle snakes venom by extracts and constituents of the plant Eclipta prostrata (Asteraceae). Toxicon 27: 1003-1009.
• Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473-497.
• Murphy RC, Hammarstrom S, Samuelsson B. 1979. Leukotriene C: a slow-reacting substance from murine mastocytoma cells. Proc Natl. Acad. Sci. USA 76: 4275-4279.
• Nunes E d-C, de Castilho CV, Moreno FN, Viana AM. 2002 In vitro culture of Cedrela fissilis vellozo (Meliaceae). Plant Cell Tiss. Organ Cult. 70: 259-268.
• Patil VM. 1998. Micropropagation studt es in Ceropegia spp. In vitro Cell Dev. Biol. Plant. 34: 240-243.
• Patnaik SK, Chand PK. 1996. In vitro propagation of the medicinal herbs Ocimum americanum L. syn, O. canum sims (hoary basil) and Ocimum sanctum L.(holy basil). Plant Cell Rep. 15: 846-850.
• Purohit SD, Dave A, Kukda G. 1994. Micropropgation of safed musli (Chlorophytum borivilianum), a rare medicinal herb. Plant Cell Tiss. Organ Cult. 39: 93-96.
• Sahoo Y, Chand PK. 1998. Micropropagation of Vitex negundo L. a woody aromatic medicinal shrub, through high frequency axillary shoot protiferation. Plant Cell Rep. 18: 301-307.
• Sen J, Sharma AK. 1991 Micropropagation of Withania somnifera from germinating seeds and shoot tips. Plant Cell Tiss. Organ Cult. 26: 71-73.
• Sreekumar S, Seeni S, Pushpangadan P. 2000. Micropropagation of Hemidesmus indicus for cultivation and production of 2-hydroxy 4 methoxy benzalt dehyde. Plant Cell Tiss. Organ Cult. 62: 211-218.
• Wagner H, Geyer B, Kiso Y, Rao GS. 1986. Coumestans as the main active principles of liver drugs Eclipta alba, Wedelia calendulaceae Planta Med. 52: 370-373.
• Yahara S, Ding N, Nohara T, Masuda K, Ageta H. 1997. Taraxtane glycosides from Eclipta alba. Phytochemistry 44: 131-135.
• Yang J, Hu Z, Guo G Q, Zheng GC. 2001 In vitro plant regeneration from cotyledon explants of Swainsona salsula Taubert, Plant Cell Tiss. Organ Cult. 66: 35-39.