Zastosowanie elektroforezy w zelu poliakryloamidowym [SDS-PAGE] do oceny struktury czasteczek IgG - glownego skladnika preparatow ludzkich immunoglobulin do stosowania dozylnego

• Autorzy: Kwas M. , Bucholc B. , Slusarczyk J.
• Języki publikacji: PL

Abstrakty

PL

Zastosowano elektroforezę w żelu poliakryloamidowym (SDS-PAGE) do oceny stopnia strukturalnej integralności cząsteczek IgG w preparacie immunoglobuliny do stosowania dożylnego.

EN

For the assessment of the structure of IgG particles in the immunoglobulin preparation for intravenous use (Bioglobulin) electrophoresis in Polyacrylamide gel (SDS - PAGE) was applied in place of filtration on Sephadex G - 200 gel. The method serves for determination of the molecular weight of polypeptide chains under conditions of reduction. The method is rapid, sensitive and of high resolution. Six batches of the preparation were examined finding that IgG monomers and dimers accounted for over 90%, with IgG aggregates about 3% and degradation products about 1%. The obtained values comply with the recomendation of the WHO and requirements in the latest edition of Europea Pharmacopaes.

Słowa kluczowe

PL

choroby autoimmunologiczne; immunoglobuliny; choroby zakazne; zel poliakrylamidowy; profilaktyka; leczenie; elektroforeza

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2000
• Tom: 52
• Numer: 3
• Opis fizyczny: s.301-309,fot.,rys.,tab.,bibliogr.

Twórcy

autor

Kwas M.

• Panstwowy Zaklad Higieny, 00-791 Warszawa, ul.Chocimska 24

autor

Bucholc B.

autor

Slusarczyk J.

Bibliografia

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