PCo-A analysis of strawberry germplasm used in European breeding programs, based on evaluation of DNA polymorphism of investigated plants

• Autorzy: Kuras A , Korbin M.

Warianty tytułu

PL

Analiza odmian truskawki uzywanych w europejskich programach hodowlanych metoda PCo-A, na podstawie oceny polimorfizmu DNA badanych roslin

• Języki publikacji: EN

Abstrakty

EN

The level of genetic relatedness of ninety-six strawberry cultivars, released in dif­ferent breeding centres of seventeen countries, was estimated based on analysis of their DNA polymorphism. Five hundred fifty-eight polymorphic amplicons, with a size range from 80 to 2600 bp, were generated in PCRs carried out on the template of DNA isolated from plants representing all analyzed cultivars. In RAPD reactions, polymorphic bands covered 58% of the total number of PCR products, while in ISSR, SSR and selective AFLP, the polymorphic DNA fragments covered 75%, 70% and 67% of all amplicons, respectively. Data concerning DNA polymorphism were as­sembled using the PCo-A method (Principal Component Analysis), and then referred to information about country of origin and pedigree described by the breeders. The results showed that contemporary breeding uses genetic resources in a very narrow range. Consequently, the cultivars released in individual breeding centres presented a very close relationship and were grouped in one, or at most two, genetic clusters.

PL

Stopień pokrewieństwa 96 odmian truskawki, wytworzonych w różnych o środ­kach hodowlanych w 17 państwach, oceniono na podstawie wyników badań polimor­fizmu analizowanych roślin. W reakcjach amplifikacji na matrycy DNA z analizowa­nych genotypów wygenerowano 558 polimorficznych amplikonów o wielkości od 80 do 2600 pz. W reakcjach RAPD polimorficzne fragmenty DNA stanowiły 58% łącz­nej liczby produktów PCR, w reakcjach ISSR - 75%, SSR - 70%, a w reakcjach selektywnych AFLP - 67%. Dane dotyczące polimorfizmu DNA zgrupowano, uży­waj ąc metody PCo-A (analiza składników głównych) i odniesiono do dostępnych informacji na temat rodowodu i kraju pochodzenia odmiany. Uzyskane wyniki wska­zuj ą, że generalnie współczesna hodowla korzysta z bardzo wąskiej puli materiałów wyjściowych. W konsekwencji, analizowane odmiany wykazał' bardzo bliskie po- krewie ństwo i w przypadku poszczególnych ośrodków hodowlanych był' zgroma­dzone w pojedynczych, a maksymalnie w dwóch skupieniach.

Słowa kluczowe

EN

genetic relatedness; Fragaria x ananassa; strawberry; plant cultivar; DNA polymorphism; principal component analysis; germplasm; European breeding programme; evaluation

• Wydawca: -
• Czasopismo: Journal of Fruit and Ornamental Plant Research
• Rocznik: 2010
• Tom: 18
• Numer: 2
• Opis fizyczny: p.7-16,fig.,ref.

Twórcy

autor

Kuras A

• Research Institute of Pomology and Floriculture, Pomologiczna 18, 96-100 Skierniewice, Poland

autor

Korbin M.

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