Use of the post-insertion method for the formation of ligand-coupled liposomes

• Autorzy: Allen T M , Sapra P. , Moase E.
• Języki publikacji: EN

Abstrakty

EN

A new technique is described for the formation of ligand-targeted liposomes that can be used with whole antibodies, antibody fragments, peptides or other ligands. The ligands are coupled to polyethylene glycol micelles and then transferred in a simple incubation step from the micelles into the outer monolayer of pre-formed, drug-loaded liposomes, This versatile method allows a combinatorial approach to the design of targeted liposomes that minimises manufacturing complexities, allowing a variety of ligands to be inserted into a variety of pre-formed liposomes containing a variety of drugs. This allows the ligand-targeted therapeutics to be tailored to the needs of individual patients.

Słowa kluczowe

EN

liposome; formation; vincristine; doxorubicin; immunoliposome; ligand-coupled liposome; polyethylene glycol; post-insertion method; antibody

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 3
• Opis fizyczny: p.889-894,fig.

Twórcy

autor

Allen T M

• University of Alberta, Edmonton, Alberta, Canada T6G 2H7

autor

Sapra P.

autor

Moase E.

Bibliografia

• 1. Hansen, C. B., Kao, G. Y., Moase, E. H., Zalipsky, S., Allen and T. M. Attachment of antibodies to sterically stabilized liposomes: evaluation, comparison and optimization of coupling procedures. Biochim. Biophys. Acta 1239 (1995) 133-144.
• 2. Allen, T. M., Hansen, C. B. and Stuart, D. D. Targeted sterically stabilized liposomal drug delivery, in Medical Applications of Liposomes (Lasic, D. D. and Papahadjopoulos, D. Eds.) 1998, Elsevier Science Publishers: Amsterdam 297-323.
• 3. Klibanov, A. L., Serbina, N., Torchilin, V. P. and Huang, L. Attachment of ligands to liposomes via PEG spacer for prolonged liposome circulation and targeting. J. Liposome Res. 6 (1996) 195-196.
• 4. Kirpotin, D., Park, J. W., Hong, K., Keller, G., Benz, C. and Paphadjopoulos, D. Binding and endocytosis of sterically stabilized anti-HER2 immunoliposomes by human breast cancer cells. Proc. Am. Assoc. Cancer Res. 37 (1996) 3186.
• 5. Zalipsky, S., Puntambekar, B., Bolikas, P., Engbers, C. M., Woodle and M. C. Peptide attachment to extremities of liposomal surface grafted PEG chains: Preparation of the long-circulating form of laminin pentapetide, YIGSR. Bioconj. Chem. 6 (1995) 705-708.
• 6. Ishida, T., Iden, D. L. and Allen, T. M. A combinatorial approach to producing sterically stabilized (Stealth) immunoliposomal drugs. FEBS Lett. 460 (1999) 129-133.
• 7. Iden, D. L. and Allen, T. M. In vitro and in vivo comparison of immunoliposomes made by conventional coupling techniques with those made by a new post-insertion technique. Biochim. Biophys. Acta 1513 (2001) 207-216.
• 8. Moreira, J. N., Ishida, T., Gaspar, R. and Allen, T. M. Use of the post-insertion technique to insert peptide ligands into pre-formed Stealth liposomes with retention of binding activity and cytotoxicity. Pharm. Res. 19 (2002) 265-269.
• 9. Bolotin, E. M., Cohen, R., Bar, L. K., Emanuel, S. N., Lasic, D. D. and Barenholz, Y. Ammonium sulphate gradients for efficient and stable remote loading of amphipathic weak bases into liposomes and ligandosomes. J. Liposome Res. 4 (1994) 455-479.
• 10. Boman, N. L., Masin, D., Mayer, L. D., Cullis, P. R., Bally and M. B. Liposomal vincristine which exhibits increased drug retention and increased circulation longevity cures mice bearing P388 tumors. Cancer Res. 54 (1994) 2830-2833.
• 11. Lopes de Menezes, D. E., Pilarski, L. M. and Allen, T. M. In vitro and in vivo targeting of immunoliposomal doxorubicin to human B-cell lymphoma. Cancer Res. 58 (1998) 3320-3330.