Non-random distribution of GATC sequences in regions of promoters stimulated by the SeqA protein of Escherichia coli

• Autorzy: Strzelczyk B. , Slominska-Wojewodzka M. , Wegrzyn G. , Wegrzyn A.
• Języki publikacji: EN

Abstrakty

EN

The SeqA protein of Escherichia coli is not only the main negative regulator of DNA replication initiation but also a specific transcription factor. It binds to hemi- methylated GATC sequences and, with somewhat different specificity, to fully meth­ylated GATC regions. Recently, a microarray analysis was reported, in which transcriptomes of wild-type and AseqA strains were compared. Although in the ΔseqA mutant the levels of some transcripts were significantly decreased while certain tran­scripts were evidently more abundant relative to wild-type bacteria, no correlation between the presence of GATC motifs in promoter sequences and transcription activ­ity was found. However, here we show that when larger DNA fragments, encompass­ing positions from -250 to +250 relative to the transcription start site, are analyzed, some common features of GATC distribution near the promoters activated by SeqA can be demonstrated. Nevertheless, it seems that the GATC pattern is not the only determinant of SeqA-dependence of promoter activity.

Słowa kluczowe

EN

transcription regulation; seqA gene; DNA replication; SeqA protein; GATC sequence; Escherichia coli; promoter sequence

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 4
• Opis fizyczny: p.941-945,fig.,ref.

Twórcy

autor

Strzelczyk B.

• University of Gdansk, Kladki 24, 80-822 Gdansk, Poland

autor

Slominska-Wojewodzka M.

autor

Wegrzyn G.

autor

Wegrzyn A.

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