Potential genotoxicity of Fusarium mycotoxins in Vicia and Pisum cytogenetic tests

• Autorzy: Packa D
• Języki publikacji: EN

Abstrakty

EN

Several fusarial toxins (DAS, DON, FUS-X and MON) were assayed for their genotoxic activity with Vicia faba var. minor and Pisum sativum used as eukaryotic, whole-organism, test systems. Four concentrations: 1, 5, 10 and 20 µg mL⁻¹ were applied for 24 hours, and three fixing times: 24, 48 and 72 hours after the beginning of the treatment. Nuclei and chromosomes in mitotic cells were stained by the Feulgen method. Generally, fusarial toxins in plant cells produced three types of effects: prevented proliferating cells from entering mitosis, caused mitotic alterations and were cytotoxic. Mitotic alterations included C-mitoses, a higher incidence of metaphases/anaphases, excessive condensation of chromosomes, multipolar spindles, disturbed anatelophases and micronuclei. Both type and range of each effect were related to plant species, toxin concentration and duration of the recovery period. The results obtained from our plant bioassays were compared with those reported from other assay systems.

Słowa kluczowe

EN

mycotoxin; cytogenetic analysis; plant cell; mitosis; cytotoxicity; gene mutation; Fusarium; chromosome; genotoxicity; fusarial toxin; mitotic cell; cytogenetic test; Vicia faba var.minor; morphological analysis; mutagenic behaviour; Pisum sativum

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1998
• Tom: 39
• Numer: 2
• Opis fizyczny: p.171-192,fig.

Twórcy

autor

Packa D

• University of Agriculture and Technology, Pl.Lodzki 3, 10-724 Olsztyn, Poland

Bibliografia

• Ames B.N., Mccann J., Yamasaki E. (1975). Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test. Mutat. Res. 31: 347-364.
• Bartholomew R.M., Ryan D.S. (1980). Lack of mutagenicity of some phytoestrogenes in the Salmonella/mammalian microsome assay. Mutat. Res. 78: 317-321.
• Beardall J.M., Miller J.D. (1994). Diseases in humans with mycotoxins as possible causes. In: Mycotoxins in grain. Compounds other than aflatoxin (J.D. Miller, H.J. Trenholm, eds.). Eagan Press. St. Paul, Minnesota, USA: 487-539.
• Bilgrami K.S. (1991). Histopathological and cytological abnormalities induced by fusarial toxins in mice (Mus musculus). Mycotoxins Res. Vol. 7, A, Part I: 12-16.
• Bradlaw J.A., Swentzel K.C., Alterman E., Hauswirth J.W. (1985). Evaluation of purified 4-deoxynivalenol (vomitoxin) for unscheduled DNA synthesis in primary rat hepatocyte-DNA repair assay. Food Chem. Toxic. 23(12): 1063-1067.
• Casale W.L., Hart L.P. (1988). Inhibition of ³H-leucine incorporation by trichothecene mycotoxins in maize and wheat tissue. Phytopathology 78(12): 1673-1677.
• Cheng S.J., Jiang Y.Z., Li M.H., Lo H.Z. (1985). A mutagenic metabolite produced by Fusarium moniliforme isolated from Linxian County, China. Carcinogenesis 6: 903-905.
• Degrassi F., Rizzoni M. (1982). Micronucleus test in Vicia faba root tips to detect damage in fresh-water pollution. Mutat. Res. 97: 19-33.
• Gelderblom W.C.A., Snyman S.D. (1991). Mutagenicity of potentially carcinogenic mycotoxins produced by Fusarium moniliforme. Mycotoxin Res. 7: 46-52.
• Gelderblom W.C.A., Thiel P.G., Marasas W.F.O., van der Merwe K.J. (1984). Natural occurrence of fusarin C, a mutagen produced by Fusarium moniliforme, in corn. J. Agric. Food Chem. 32: 1064-1067.
• Gerlach D. (1972). Zarys mikrotcchniki botanicznej. Państwowe Wydawnictwo Rolnicze i Leśne Warszawa.
• Hsia C.C., Gao Y„ Wu J.I., Tzian B.L. (1986). Induction of chromosome aberration by Fusarium T-2 toxin in cultured human peripheral blood lymphocytes and Chinese hamster fibroblasts. J. Cell. Physiol. Suppl. 4: 65-72.
• Hsia C.C., Tzian B.L., Harris C.C. (1983). Proliferative and cytotoxic effects of Fusarium T-2 toxin on cultured human fetal esophagus. Carcinogenesis 4(9): 1101-1107.
• Hsia C.C., Wu J.L., Lu X.Q., Li Y.S. (1988). Natural occurrence and clastogenic effects of nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol,15-acetyldeoxynivalenol, and zearalenone in corn from a high risk area of esophageal cancer. Cancer Detect. Prevent. 13: 79-86.
• Iасоbellis N.S., Bottalico A. (1981). Effects of some trichothccenes produced by species of Fusarium on electrolyte leakage from tomato leaf disc. Phytopath. Medit. 20: 129-132.
• Ingerowski G.H., Scheutwinkel-Reich M., Stan H.J. (1981). Mutagenicity studies on veterinary anabolic drugs with Salmonella/microsome test. Mutat. Res. 91:93-98.
• Jałoszyński P., Kujawski M., Szyfter K. (1996). Elektroforeza pojedynczych komórek (comet assay) - użyteczna technika badania uszkodzeń DNA. Post. Biol. Kom. 23(3): 339-354.
• Joffe A.Z. (1986). Fusarium species: Their biology and toxicology. John Wiley and Sons, Inc. New York.
• Kuczuk M.H., Benson P.M., Heath H., Hayes A.W. (1978). Evaluation of the mutagenic potential of mycotoxins using Salmonella typhimurium and Saccharomyces cerevisiae. Mutat. Res. 53: 11-20.
• Lafarge-Frayssinet С., Decloitre F., Mousset S., Martin M., Frayssinet C. (1981). Induction of DNA single-strand breaks by T-2 toxin, a trichothecene metabolite of Fusarium. Mutat. Res. 88: 115-123.
• Linnainmaa K., Sorsa M., Ilus T. (1979). Epoxytrichothecene mycotoxins as C-mitotic agents in Allium. Hereditas 90: 151-156.
• Ma T.H. (1982a). Vicia cytogenetic tests for environmental mutagens. Mutat. Res. 99: 257-271
• Ma T.H. (1982b). Tradescantia cytogenetic tests (root-tip mitosis, pollen mitosis, pollen mother-cell meiosis). Mutat. Res. 99: 293-302.
• Nijs de M., Rombouts F., Notermans S. (1996). Fusarium molds and their mycotoxins. J. Food Safety 16: 15-58.
• Obe G., Natarajan A.T. (eds.) (1990). Chromosomal aberrations. Basic and applied aspects. Springer-Verlag. Berlin.
• Packa D. (1997). Cytogenetic effects of Fusarium mycotoxins on root tip cells of rye (Secale cereale L.), wheat (Triticum aestivum L.) and field bean (Vicia faba L. var. minor). J. Appl. Genet. 38(3): 259-272.
• Parry E.M., Henderson L., MacKay J.M. (1995). Guidelines for testing of chemicals. Procedures for the detection of chemicals induced ancuploidy: recommendations of a UK Environmental Mutagen Society working group. Mutagenesis 10(1): 1-14.
• Pfohl-Leszkowicz A., Chekir-Ghedira L., Bacha H. (1995). Genotoxicity of zearalenone, an estrogenic mycotoxin: DNA adduct formation in female mouse tissues. Carcinogenesis 16(10): 2315-2320.
• Prelusky D.B., Rotter B.A., Rotter R.G. (1994). Toxicology of mycotoxins. In: Mycotoxins in grain. Compounds other than aflatoxin (J.D. Miller, H.L. Trenholm, eds.). Eagan Press. St. Paul, Minnesota, USA: 359-403.
• Reiss J. (1975). Mycotoxin poisoning of Allium сера root tips. II. Reduction of mitotic index and formation of chromosomal aberrations and cytological abnormalities by patulin, rubratoxin В and diacetoxyscirpenol. Cytologia 39(4): 703-708.
• Rogers C.G., Heroux-Metcalf C. (1983). Cytotoxicity and absence of mutagenic activity of vomitoxin (4-deoxynivalenol) in a hepatocyte-mediated mutation assay with V79 Chinese hamster lung cells. Cancer Letters 20: 29-35.
• Rotter B.A., Prelusky D.B., Pestka J.J. (1996). Toxicology of deoxynivalenol (vomitoxin). J. Toxicol. Environ. Health 48: 1-34.
• Sąsiadek M., Paprocka-Borowicz M., Ślęzak R. (1996). Zastosowanie technik cytogenetycznych do biologicznego monitorowania narażenia na czynniki mutagenne. Post. Biol. Kom. 23(3): 373-384.
• Scott D. (1990). Clastogenesis in vitro under extreme culture conditions. In: Chromosomal aberrations. Basic and applied aspects (G. Obe, A.T. Natarajan, eds.). Springer-Verlag, Berlin: 271-286.
• Sheu C.W., Moreland F.M., Lee J.K., Dunkel V.C. (1988). Morphological transformation of ВALB/3T3 mouse embryo cells in vitro by vomitoxin. Food Chem. Toxic. 26(3): 243-245.
• Sorsa M., Linnainmaa K., Penttila M., Ilus T. (1980). Evaluation of the mutagenicity of epoxytrichothecene mycotoxins in Drosophila melanogaster. Hereditas 92: 163-165.
• Stark A. A. (1980). Mutagenicity and carcinogenicity of mycotoxins: DNA binding as a possible mode of action. Ann. Rev. Microbiol. 34: 235-262.
• Thust R., Kneist S., Huhne V. (1983). Genotoxicity of Fusarium mycotoxins (nivalenol, fusarenon-X, T-2 toxin, and zearalenone) in Chinese hamster V79-E cells in vitro. Arch. Geschwulstforsch. 53(1): 9-15.
• Tucker J.D., Auletta A., Cimino M.C., Dearfield K.L., Jacobson-Kram D., Tice R.R., Carrano A.V. (1993). Sister-chromatid exchange: second report of the Gene-Tox program. Mutat. Res. 297: 101-180.
• Ueno Y., Kubota K. (1976). DNA-attacking ability of carcinogenic mycotoxins in recombination-deficient mutant cells of Bacillus subtilis. Cancer Res. 36: 445-451.
• Ueno Y., Kubota K., Ito T., Nakamura Y. (1978). Mutagenicity of carcinogenic mycotoxins in Salmonella typhimurium. Cancer Res. 38: 536-542.
• Ueno Y., Sato N., Ishii K., Sakai K., Tsunoda H., Enomoto M. (1973). Biological and chemical detection of trichothecene mycotoxins of Fusarium species. Appl. Microbiol. 25(4): 699-704.
• Wehner F.C., Marasas W.F.O., Thiel P.G. (1978). Lack of mutagenicity to Salmonella typhimurium of some Fusarium mycotoxins. Appl. Environ. Microbiol. 35(4): 659-662.
• Wiśniewska H.,Chełkowski J. (1994). Influence of deoxynivalenol on mitosis of root tip cells of wheat seedlings. Acta Physiol. Plant. 16(3): 159-162.
• Wojciechowski S., Chełkowski J., Kostecki M. (1995). Influence of deoxynivalenol on electrolyte leakage in cereal seedling leaves. Acta Physiol. Plant. 17(4): 357-360.
• WORLD HEALTH ORGANIZATION (1990). Environmental Health Criteria 105. Selected mycotoxins: ochratoxins, trichothecenes, ergot. Geneva: WHO.
• Zeeland van A.A. (1996). Molecular dosimetry of chemical mutagens. Relationship between DNA adduct formation and genetic changes analyzed at the molecular level. Mutat. Res. 353: 123-150.