Studies on antioxidant properties of polyphenol-rich extract from berries of Aronia melanocarpa in blood platelets

• Autorzy: Olas B. , Wachowicz B. , Nowak P. , Kedzierska M. , Tomczak A. , Stochmal A. , Oleszek W. , Jeziorski A. , Piekarski J.
• Języki publikacji: EN

Abstrakty

EN

The antioxidant properties of extract from berries of Aronia melanocarpa (chokeberry) containing: anthocyanidines, phenolic acids and quercetine glycosides on oxidative/nitrative stress induced by peroxynitrite (ONOO-, a powerful physiological oxidant, nitrating species and inflammatory mediator) in human blood platelets were studied in vitro. The extract from A. melanocarpa (5 – 50 µg/mL) significantly inhibited platelet protein carbonylation (measured by ELISA method) and thiol oxidation estimated with 5,5’-dithio-bis(2-nitro-benzoic acid) (DTNB) induced by peroxynitrite (0.1 mM) (IC50 - 35 µg/mL for protein carbonylation, and IC50 - 33 µg/mL for protein thiol oxidation). The tested extract only slightly reduced platelet protein nitration (measured by C- ELISA method). The extract also caused a distinct reduction of platelet lipid peroxidation induced by peroxynitrite. Moreover, in our preliminary experiments we observed that the extract (50 µg/mL) reduced oxidative/nitrative stress in blood platelets from patients with breast cancer. The obtained results indicate that in vitro the extract from A. melanocarpa has the protective effects against peroxynitrite – induced oxidative/nitrative damage to the human platelet proteins and lipids. The extract from A. melanocarpa seems to be also useful as an antioxidant in patients with breast cancer.

Słowa kluczowe

EN

peroxynitrite; plant extract; oxidative stress; chokeberry; anthocyanidine; Aronia melanocarpa; antioxidative property; antioxidant; platelet; phenolic acid; berry

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2008
• Tom: 59
• Numer: 4
• Opis fizyczny: p.823-835,fig.,ref.

Twórcy

autor

Olas B.

• University of Lodz, Banacha 12/16, 90-237 Lodz, Poland

autor

Wachowicz B.

autor

Nowak P.

autor

Kedzierska M.

autor

Tomczak A.

autor

Stochmal A.

autor

Oleszek W.

autor

Jeziorski A.

autor

Piekarski J.