The immune response against gastrointestinal nematodes in naturally infected Polish Wrzosowka sheep. I. The serum IgG response to Haemonchus contortus in ewes over three grazing seasons

• Autorzy: Moskwa B
• Języki publikacji: EN

Abstrakty

EN

Faecal nematode egg counts and IgG activity to Haemonchus contortus were followed in naturally infected Polish Wrzosówka ewes. The egg counts were overdis- persed; most individuals had relatively low egg counts but a small proportion had high counts. Egg production followed a different pattern each grazing season. Larvae cultured from eggs in 1996 were predominantly H. contortus and Trichostrongylus spp. In 1997 the recoveries were largely Trichostrongylus spp. In 1998, larval recoveries were mainly Trichostrongylus spp. and Teladorsagia spp. There were no discernible patterns in the composition of the nematode population within each grazing season. Egg counts in different months were moderately repeatable, indicating that animals tended to maintain their rankings over time. The repeatabilities rose during the grazing season but declined with increasing intervals between sample dates. IgG activity against a somatic extract of adult H. contortus was higher than activity against a preparation from excretory/secretory antigens but the two responses were very strongly correlated. The repeatability of IgG activity at monthly intervals was higher than the repeatability of faecal egg counts. Animals with higher than average IgG activity had significantly lower than average egg counts but only in the last two years of the study. A combination of egg counts and antibody responses may be better at identifying resistant animals than either method used in isolation, but more research is necessary to determine why the association between antibody and egg counts varies in different years.

Słowa kluczowe

EN

antigen; grazing season; infection; serum; sheep; gastrointestinal nematode; ELISA test; Haemonchus contortus; ewe; Polish Wrzosowka sheep; nematode; immune response

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1999
• Tom: 44
• Numer: 4
• Opis fizyczny: p.266-273,fig.

Twórcy

autor

Moskwa B

• Polish Academy of Sciences, Twarda 51-55, 00-818 Warsaw, Poland

Bibliografia

• Adams D. B., Davies H. I. 1982. Enhanced resistance to infection with Haemonchus contortus in sheep treated with a corticosteroid. International Journal for Parasitology, 12, 523-529.
• Anonymous 1986. Manual of veterinary parasitological techniques. Ministry of Agriculture, Fisheries and Food, Her Majesty's Stationary Office, London.
• Bairden K. 1991. Ruminant parasitic gastritis; some observations on epidemiology and control. PhD Thesis, University of Glasgow.
• Baker R. L., Watson T. G., Bisset S. A., Vlassoff A., Douch P. G. 1991. Breeding sheep in New Zealand for resistance to internal parasites: research results and commercial applications. In: Breeding for disease resistance in sheep (Eds. G. D. Gray and R. R. Woolaston). Wool Research and Development Corporation, Parkville, Australia, 19-32.
• Barger I. A., Le Jambre L. F., Georgi J. R, Davies H. I. 1985. Regulation of Haemonchus contortus populations in sheep exposed to continuous infection. International Journal for Parasitology, 15, 529-533.
• Bishop S. C., Bairden K., McKellar Q. A., Park M., Stear M. J. 1996. Genetic parameters for faecal egg counts following mixed, natural, predominantly Ostertagia circumcincta infection and relationships with live weight in young lambs. Animal Science, 63, 423-428.
• Bisset S. A., Vlassoff A., Morris C. A., Southey B. R., Baker R. L. 1992. Heritability of and genetic correlations among faecal egg counts and productivity traits in Romney sheep. New Zealand Journal of Agricultural Research, 35, 51-58.
• Bouix J., Krupiński J., Rzepecki R., Nowosad B., Skrzyzala I., Roborzynski M., Fudalewicz-Niemczyk W., Skalska M., Malczewski A., Gruner L. 1998. Genetic resistance to gastrointestinal nematode parasites in Polish long-wool sheep. International Journal for Parasitology, 28, 1797-1804.
• Doligalska M., Moskwa B., Niżnikowski R. 1997. The repeatability of faecal egg counts in Polish Wrzosówka sheep. Veterinary Parasitology, 70, 241-246.
• Douch P. G. C., Green R. S., Risdon P. L. 1994. Antibody responses of sheep to challenge with Trichostrongylus colubriformis and the effect of dexamethasone treatment. International Journal for Parasitology, 24, 921-928.
• Douch P. G. C., Green R. S., Morris C. A., McEwan J. C., Windon R. G. 1996. Phenotypic markers for selection of nematode-resistant sheep. International Journal for Parasitology, 26, 899-911.
• Fahmy M. H. 1998. Prolific sheep. CAB International, Wallingford. University Press, Cambridge, UK.
• Gill H. S., Gray G. D., Watson D. L., Husband A. J. 1993a. Isotype- specific antibody responses to Haemonchus contortus in genetically resistant sheep. Parasite Immunology, 15, 61-67.
• Gill H. S., Watson D. L., Brandon M. R. 1993b. Monoclonal antibody to CD4⁺ T cells abrogates genetic resistance to Haemonchus contortus in sheep. Immunology, 78, 43-49.
• Green R. S., Shirer C. L., Douch P. G. C. 1994. Dynamic of antibody production in parasite-naive responsive sheep. New Zealand Journal of Zoology, 21, 102.
• Green R. S., Douch P. G. C., Hill F. I., Deatch A. F., Weth T. K., Donaghy M. J. 1996. Antibody responses of grazing alpacas (Lama pacos) in New Zealand to intestinal nematodes. International Journal for Parasitology, 26, 429-435.
• Jacobs H. J., Wiltshire C., Ashman K., Meeusen E. N. T. 1999. Vaccination against the gastrointestinal nematode, Haemonchus contortus, using a purified larval surface antigen. Vaccine, 17, 362-368.
• Knox D. P., Jones D. G. 1990. Studies on the presence and release of proteolytic enzymes (proteinases) in gastro-intestinal nematodes of ruminants. International Journal for Parasitology, 20, 243-249.
• Lowry O. H., Rosenbrough N. J., Farr A. L., Randall R. L. 1951. Protein measurement with Folin phenol reagent. Journal of Biological Chemistry, 193, 265-275.
• Malczewski A. 1970. Gastro-intestinal helminths of ruminants in Poland. I. Helminth fauna of sheep. Acta Parasitologica Polonica, 17, 245-254.
• Mitchell L. 1996. Clean grazing for parasite control. Farmer and Grower, 52, 8-11.
• Morris C. A., Vlassoff A., Bisset S. A., Baker R. L., West C. J., Hurford A. P. 1997. Responses of Romney sheep to selection for resistance or susceptibility of nematode infection. Animal Science, 64, 319-329.
• Moskwa B., Charon K. M., Cabaj W., Rutkowski R. 1998a. The mean, distribution and repeatability of faecal egg counts in Polish Wrzosówka ewes following natural gastrointestinal nematode infection during two pasture seasons. Acta Parasitologica, 43, 94-99.
• Moskwa B., Doligalska M., Cabaj W. 1998b. The repeatability of haematological and parasitological parameters in Polish Wrzosówka hoggets naturally infected with trichostrongylid nematodes. Acta Parasitologica, 43, 148-153.
• Pfeffer A., Douch P. G. C., Shaw R. J., Gatehouse T. K., Rabel B., Green R. S., Shirer C. L., Jonas W. E., Bisset S. 1996. Sequential cellular and humoral responses in the abomasal mucosa and blood in Romney sheep dosed with Trichostrongvlus axei. International Journal for Parasitology, 26, 765-773.
• Schallig H. D. F. H., van Leeuwen M. A. W., Hendrikx W. M. L. 1995. Isotype-specific serum antibody responses of sheep to Haemonchus contortus antigens. Veterinary Parasitology, 56, 149-162.
• Siński E., Bairden K., Duncan J. L., Eisler M. C., Holmes P. H., McKellar Q. A., Murray M., Stear M. J. 1995. Local and plasma antibody responses to the parasitic larval stages of the abomasal nematode Ostertagia circumcincta. Veterinary Parasitology, 59, 107-118.
• Smith W. D., Jackson F., Jackson E., Williams J. 1985. Age immunity to Ostertagia circumcincta: comparison of the local immune responses of 4½ and 10-month-old lambs. Journal of Comparative Pathology, 95, 235-245.
• Stankiewicz M., Cabaj W., Pernthaner A., Jonas W., Rabel B. 1996. Drug-abbreviated infections and development of immunity against Trichostrongylus colubriformis in sheep. International Journal for Parasitology, 26, 97-103.
• Stear M. J., Murray M. 1994. Genetic resistance to parasitic disease: particularly of resistance in ruminants to gastrointestinal nematodes. Veterinary Parasitology, 54, 161-176.
• Stear M. J., Bishop S. C., Duncan J. L., McKellar Q. A., Murray M. 1995. The repeatability of faecal egg counts peripheral eosinophil counts, and plasma pepsinogen concentration during deliberate infections with Ostertagia circumcincta. International Journal for Parasitology, 25, 375-380.
• Stear M. J., Bairden K., Bishop S. C., Buitkamp J., Duncan J. L., Gettinby G., McKellar Q. A., Park M., Parkins J. J., Eid S.W. J., Murray M. 1997. The genetic basis of resistance to Ostertagia circumcincta in lambs. Veterinary Journal, 154, 111-119.
• Strain S. A. J., Stear M. J. The influence of protein supplementation on the immune response to Haemonchus contortus. Parasite Immunology, (in press).
• Woolaston R. R., Baker R. L. 1996. Prospects of breeding small ruminants for resistance to internal parasites. International Journal for Parasitology, 26, 845-855.