Nitrate non-utilizing mutants and vegetative compatibility groups of Fusarium proliferatum and F. sacchari isolated from rice in the Peninsular Malaysia and Kalimantan, Indonesia

• Autorzy: Zainudin N A I M , Ismail N.A. , Nor N.M.I.M. , Razak A.A. , Sidique S.N.M. , Salleh B.

Warianty tytułu

PL

Mutanty nie zuzywajace azotu oraz grupy zgodnosci wegetatywnej Fusarium proliferatum i F. sacchari wyizolowane z ryzu na Polwyspie Malezyjskim i Kalimantan w Indonezji

• Języki publikacji: EN

Abstrakty

EN

A total of 26 isolates of Fusarium proliferatum and F. sacchari were isolated from rice in the Peninsular Malaysia and Kalimantan, Indonesia. Spontaneous chlorate-resistant sectors (CRSs) were recovered from all wild type of both Fusarium species when cultured on two chlorate media. The non-utilizing (nit) mutants were generated as crn (chlorate resistant, nitrate utilizing), nit1, nit3 and nitM based on phenotyping growth-types on diagnostic media with different sources of nitrogen. The nit mutants were paired on minimal medium (MM) for examining the vegetative compatibility. The majority of nit mutants (32.3-46.5%) recovered were nit1. Eight and seven vegetative compatibility groups (VCGs) of F. proliferatum and F. sacchari were identified, respectively. The isolates of F. proliferatum and F. sacchari were genetically diverse as shown by the number and distribution of the VCGs. No strong correlation was observed between VCGs of both species and location.

PL

Na Półwyspie Malezyjskim i w Kalimantan (Indonezja) wyizolowano ogółem 26 izolaty Fusarium proliferatum i F. sacchari z ryżu. W hodowli na dwóch pożywkach chloranowych uzyskano spontanicznie powstające sektory ze wszystkich dzikich izolatów obydwu gatunków Fusarium. Otrzymano mutanty nie zużywające azotanu (nit 1, nit 3 i nit M), a także odporne na chloran mutanty zużywające azotan, w oparciu o cechy typu wzrostu, na pożywkach diagnostycznych z różnymi źródłami azotu. Mutanty nit łączono parami na pożywce mineralnej (MM) w celu sprawdzenia zgodności wegetatywnej. Większość mutantów nit (32,3-46,5%) określono jako nit 1. Zidentyfikowano 8 i 7 grup zgodności wegetatywnej odpowiednio dla F. proliferatum i F. sacchari. Izobaty F. proliferatum i F. sacchari były różne pod względem genetycznym, co wykazano na podstawie liczby i rozmieszczenia grup zgodności wegetatywnej. Nie stwierdzono wysokiej korelacji pomiędzy grupami zgodności wegetatywnej w ramach tych gatunków oraz miejscowości.

Słowa kluczowe

EN

isolation; Fusarium sacchari; nitrate non-utilizing mutant; Kalimantan; Malaysia; Fusarium proliferatum; rice; vegetative compatibility group; Indonesia

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2009
• Tom: 49
• Numer: 2
• Opis fizyczny: p.229-235,fig.,ref.

Twórcy

autor

Zainudin N A I M

• Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

autor

Ismail N.A.

autor

Nor N.M.I.M.

autor

Razak A.A.

autor

Sidique S.N.M.

autor

Salleh B.

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