The effect of antisense oligonucleotide treatment of plasma membrane Ca2plus-ATPase in PC12 cells

• Autorzy: Szemraj J , Kawecka I. , Bartkowiak J. , Zylinska L.
• Języki publikacji: EN

Abstrakty

EN

Plasma membrane Ca2+-ATPase (PMCA), encoded by four separate genes, constitutes a high affinity system extruding Ca2+ outside the cell. The nerve growth factor-treated PC12 cell line possesses all four main PMCA isoforms. To evaluate the potential role of PMCA isoforms in the differentiation process, we transiently suppressed the expression of PMCA2 and 3 using the antisense oligonucleotides. In the transfected PC12 cells, we observed morphological changes, slowed neurite extension and diminished survival of the cells. The apparent transport activity and affinity of the calcium pump to Ca2+ were lower in the cells with suppressed PMCA2 and 3 isoforms than in the control cells. Moreover, in the transfected PC12 plasma membranes, the calcium pump was insensitive to stimulation by calmodulin. These findings suggest that PMCA2 and 3 isoforms may be involved in developmental and differentiation processes.

Słowa kluczowe

EN

antisense oligonucleotide; calcium; cell physiology; ATPase; isoform; PC12 cell

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2004
• Tom: 09
• Numer: 3
• Opis fizyczny: p.451-464,fig.,ref.

Twórcy

autor

Szemraj J

• Medical University, Mazowiecka 6-8, 92-215 Lodz, Poland

autor

Kawecka I.

autor

Bartkowiak J.

autor

Zylinska L.

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