EN
A new ELISA method for VHDV diagnosis (indirect sandwich ELISA) and Iiquid-phase blocking ELISA (LPBE) for the determination of antibodies to VHDV were developed. These assays are sensitive and specific and results may be directly correlated to those recorded by HA or HI. Besides, the presented techniques are rapid and relatively simple to perform and reagents are used economically. Therefore, the described immunoassays can be introduced to laboratory practice for the primary diagnosis of VHD.