Endotoxin levels as a potential marker of concentration of Gram-negative bacteria in water effluent from dental units and dental aerosols

• Autorzy: Szymanska J.
• Języki publikacji: EN

Abstrakty

EN

Gram-negative bacteria concentration in water effluent from a dental unit, and in dental aerosol forming during the work of a dental handpiece, was assessed. The study was conducted on 25 dental units before and after a 2-week period of using a disinfecntant for water in dental units waterlines (DUWL). The contamination of water with Gram-negative bacteria before disinfection was 18-398 x 103 cfu/ml, and after disinfection, bacteria were not found. The concentration of Gram-negative bacteria in the air before disinfection was 0-23 x 101 cfu/m3, and after disinfection - 0-8 x 101 cfu/m3. Simultaneously, the water and air were sampled to determine bacterial endotoxin. The statistical analysis did not show correlation between endotoxin concentration and Gram-negative bacteria concentration for the water before disinfection, and for the air before and after disinfection of DUWL water. Because the number of bacteria in the water after disinfection dropped to zero, statistical methods could not be used. The performed analysis suggests that bacterial endotoxin concentration is not indicative of Gram-negative bacteria contamination. Thus, bacterial endotoxin determination is not recommended as a method of monitoring the microbiological quality of DUWL water and dental aerosols.

Słowa kluczowe

EN

bacteria; Gram-negative bacterium; endotoxin; dental unit waterline; water; aerosol; microbiological quality

• Wydawca: -
• Czasopismo: Annals of Agricultural and Environmental Medicine
• Rocznik: 2005
• Tom: 12
• Numer: 2
• Opis fizyczny: p.229-232,ref.

Twórcy

autor

Szymanska J.

• Medical Universitu of Lublin, Staszica 11, 20-018 Lublin, Poland

Bibliografia

• 1. Dutkiewicz J, Śpiewak R, Jabłoński L: Klasyfikacja Szkodliwych Czynników Biologicznych Występujących w Środowisku Pracy oraz Narażonych na nie Grup Zawodowych. 3rd Ed. Ad Punctum, Lublin 2002.
• 2. Fulford MR, Walker JT, Martin MV, Marsh PD: Total viable counts, ATP, and endotoxin levels as potential markers of microbial contamination of dental unit water systems. Br Dent J 2004, 196, 157- 159.
• 3. Górny RL: Biologiczne czynniki szkodliwe: normy, zalecenia i propozycje wartości dopuszczalnych. Podstawy i Metody Oceny Środowiska Pracy 2004, 41(3), 17-39.
• 4. Górny LR, Dutkiewicz J: Bacterial and fungal aerosols in indoor environment in Central and Eastern European countries. Ann Agric Environ Med 2002, 9, 17-23.
• 5. Harrel SK, Molinari J: Aerosols and splatter in dentistry: a brief review of the literature and infection control implications. J Am Dent Assoc 2004, 135, 429-437.
• 6. Krieg NR, Holt JG (Eds): Bergey's Manual of Systematic Bacteriology. Vol. 1. Williams &Wilkins. Baltimore 1984.
• 7. Leggat PA, Kedjarune U: Bacterial aerosols in dental clinic: a review. International Dent J 2001, 51, 39-44.
• 8. Malmros P Sigsgaard T, Bach B: Occupational health problems due to garbage sorting. Waste Manag Res 1992,10, 227-234.
• 9. Michel O, Kips J, Duchateau J, Vertongen F, Robert L, Collet H, Pauwels R, Sergysel R: Severity of asthma is related to endotoxin in house dust. Am J Respir Crit Care Med 1996, 154, 1641-1646.
• 10. Ormstad H, Groeng EC, Duffort O, Lovik M: The effect of endotoxin on the production of IgE, IgG 1 and IgG2a antibodies against the cat allergen Fel d 1 in mice. Toxicology 2003, 188, 309-318.
• 11. Putnins EE, Di Giovanni D, Bhullar AS: Dental unit waterline contamination and posisible implications during periodontal surgery. J Periodontol 2001, 72, 393-400.
• 12. Puttaiah R, Cederberg RA: Assessment of endotoxin levels in dental unit effluent water, http://www.pall.com/sls_6463.asp
• 13. Sneath PHA, Mair N, Sharpe ME, Holt JF (Eds): Bergey's Manual of Systematic Bacteriology. Vol. 2. Williams & Wilkins, Baltimore 1986.
• 14. Szymańska J: Biofilm and dental unit waterlines. Ann Agric Environ Med 2003, 10, 151-157.
• 15. Szymańska J: Control methods of microbial water quality in dental unit waterlines. Ann Agric Environ Med 2003,10, 1-4.
• 16. Szymańska J: Exposure to bacterial endotoxin during conservative dental treatment. Ann Agric Environ Med 2005, 12, 137-139.
• 17. Szymańska J. Occupational hazards of dentistry. Ann Agric Environ Med 1999, 6, 13-19.
• 18. Szymańska J. Risk to exposure to Legionella in dental practices. Ann Agric Environ Med 2004,11,9-12.
• 19. Szymańska J, Wdowiak L, Puacz E, Stojek NM: Microbial quality of water in dental unit reservoirs. Ann Agric Environ Med 2004, 11, 355-358.
• 20. Todar K: Mechanisms of bacterial pathogenicity: endotoxins. University of Wisconsin-Madison Department of Bacteriology. http://textbookofbacteriology.net/endotoxin.html
• 21. Walker JT, Bradshaw DJ, Fulford MR, Marsh PD: Microbiological evaluation of a range of disinfectant products to control mixed- species biofilm contamination in a laboratory model of a dental unit water system. Appl Environ Microbiol 2003, 69(6), 3327-3332.
• 22. Williams ST, Sharpe ME, Holt JG (Eds): Bergey's Manual of Systematic Bacteriology. Vol. 4. Williams &Wilkins, Baltimore 1989.