Analysis of high-pressure-induced disruption of human erythrocytes by flow cytometry

• Autorzy: Yamaguchi T , Terada S.
• Języki publikacji: EN

Abstrakty

EN

High-pressure-induced hemolysis is suppressed by pretreating human erythrocytes at 49°C, or enhanced by pretreatment with trypsin. So, the response of these pretreated cells to a pressure of 200 MPa was examined using flow cytometry. In the case of intact erythrocytes, a major product was fragmented particles. From 49°C-pretreated cells, vesicles were mainly released. Trypsin-pretreated cells mainly produced open ghosts. Additionally, intact erythrocytes, 49°C-pretreated ones, and trypsin-pretreated ones also released at 200 MPa vesicles of diameter 464 ± 9, 259 ± 18, and 574 ± 16 nm, respectively. These results suggest that mother cells, fragmented particles, vesicles, and open ghosts from 200 MPa-treated erythrocytes are easily monitored by flow cytometry and that the size of released vesicles may also be an important factor in high-pressure-induced hemolysis.

Słowa kluczowe

EN

membrane component; high pressure; man; interaction; erythrocyte; human erythrocyte; flow cytometry; hemolysis

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2003
• Tom: 08
• Numer: 4
• Opis fizyczny: p.1013-1016,fig.

Twórcy

autor

Yamaguchi T

• Fukuoka University, Jonan-ku, Fukuoka, 814-0180 Japan

autor

Terada S.

Bibliografia

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• 2.Yamaguchi, T., Matsumoto, M. and Kimoto, E. Effects of anion transport inhibitors on hemolysis of human erythrocytes under hydrostatic pressure. J. Biochem. 118 (1995) 760-764.
• 3.Yamaguchi, T., Terada, S. and Ideguchi, H. High-pressure-induced hemolysis of hereditary spherocytic erythrocytes is not suppressed by DIDS labeling. Jpn. J. Physiol. 47 (1997) 571-574.
• 4.Yamaguchi, T., Matsumoto, M. and Kimoto, E. Hemolytic properties under hydrostatic pressure of neuraminidase- or protease-treated human erythrocytes. J. Biochem. 114 (1993) 576-581.