Detection of bovine herpesvirus 1 in bull semen by PCR

• Autorzy: Rola J , Zmudzinski J.F.
• Języki publikacji: EN

Abstrakty

EN

The results of PCR were compared with the results of virus isolation test. 44 bovine semen samples were tested. The presence of BHV 1 was demonstrated with the virus isolation test in 6 samples of semen. PCR with external primers detected BHV 1 in 8 samples, while in nested PCR positive amplification was observed in 21 samples. The sensitivity of PCR with external primers and nested PCR were 0.6 and 0.06 of TCID₅₀ respectively.

Słowa kluczowe

EN

semen; virus isolation test; cattle; bovine herpesvirus 1; bull; polymerase chain reaction; cell culture

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1999
• Tom: 43
• Numer: 2
• Opis fizyczny: p.119-123,fig.

Twórcy

autor

Rola J

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Zmudzinski J.F.

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