Wplyw kurkuminy na detoksykacyjne i antyoksydacyjne mechanizmy w watrobie szczurow podczas starzenia

• Autorzy: Gutowicz M , Cholojczyk M. , Pyrzanowska J. , Widy-Tyszkiewicz E. , Baranczyk-Kuzma A.

Warianty tytułu

EN

Effect of curcumin on antioxidant and detoxification mechanisms in the livers of aging rats

• Języki publikacji: PL

Abstrakty

EN

Curcumin (diferuloylmethane), a yellow poliphenol pigment isolated from rhizome of Curcuma longa, has been used throughout Asia for several thousands of years. Beside giving curry dishes their color, curcumin has been reported as an agent used in the treatment of various disorders. The aim of this study was to determine the effect of chronic curcumin administration on enzymatic and nonenzymatic protective mechanisms in the livers of aging rats. The liver was isolated from Wistar Albino Glaxo rats, age 6 and 22 months. All rats were fed a standard chow diet. Two groups of older animals were given a standard diet supplemented either with 10 mg/kg b.w. or with 50 mg/kg b.w. of curcumin powder for 10 weeks before their sacrifice. Malondialdehyde level, reduced glutathione, the activity of catalase, glutathione S-transferase, and arginase were determined. Increased lipid peroxidation was observed in old rat livers, elevated catalase and arginase activities, as well as the level of reduced glutathione in curcumin fed animals. No changes in glutathione S-transferase activity were found. It may be concluded that curcumin administered in a diet exerts an antioxidant effect and protects the liver against ammonia intoxication.

Słowa kluczowe

PL

zwierzeta doswiadczalne; szczury; zwierzeta stare; starzenie sie; watroba; stres oksydacyjny; kurkumina; mechanizmy antyoksydacyjne; mechanizmy detoksykacyjne

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 07
• Opis fizyczny: s.955-957,rys.,tab.,bibliogr.

Twórcy

autor

Gutowicz M

• Akademia Medyczna w Warszawie, ul.Banacha 1, 02-097 Warszawa

autor

Cholojczyk M.

autor

Pyrzanowska J.

autor

Widy-Tyszkiewicz E.

autor

Baranczyk-Kuzma A.

Bibliografia

• 1. Bala K., Tripathy B. C., Sharma D.: Neuroprotective and anti-ageing effects of curcumin in aged rat brain regions. Biogerontology 2006, 7, 81-89.
• 2. Bradford M. M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.
• 3. Chinard F. P.: Photometric estimation of prolin and ornithine. J. Biol. Chem. 1952, 199, 91-95.
• 4. Devasena T., Rajasekaran K. N., Menon V. P.: Bis-1,7-(2-hydroxyphenyl)-hepta-1,6-diene-3,5-dione (a curcumin analog) ameliorates DMH-induced hepatic oxidative stress during colon carcinogenesis. Pharmacol. Res. 2002, 46, 39-45.
• 5. Frank N., Knauft J., Amelung F., Nair J., Wesch H., Bartsch H.: No prevention of liver and kidney tumors in Long-Evans Cinnamon rats by dietary curcumin, but inhibition at other sites and of metastases. Mutation Res. 2003, 523-524, 127-135.
• 6. Goel A., Boland C. R., Chauhan D. P.: Specific inhibition of cyclooxygenase-2 (COX-2) expression by dietary curcumin in HT-29 human colon cancer cells. Cancer Lett. 2001, 172, 111-118.
• 7. Góth L.: A simple method for determination of serum catalase activity and revision of reference range. Clin. Chim. Acta. 1991, 196, 143-152.
• 8. Gutowicz M., Sadurska B., Chołojczyk M., Pokorska-Lis M., Siwińska-Ziółkowska A., Barańczyk-Kuźma A.: Antioxidant status in different regions of heroin addicts' brain. Environ. Toxicol. Pharmacol. 2006, 21, 80-85.
• 9. Habig W. H., Pabst M. J., Jakoby W. B.: Glutathione S-transferases; the first enzymatic step in mercapturic acid formation. J. Biol. Chem. 1974, 249, 7130-7139.
• 10. Jaruga E., Bielak-Żmijewska A., Sikora E., Skierski J., Radziszewska E., Piwocka K., Bartosz G.: Glutathione-independent Mechanism of Apoptosis Inhibition by Curcumin in Rat Thymocytes. Biochem. Pharmacol. 1998, 56, 961-965.
• 11. Kelloff G. J., Crowell J. A., Hawk E. T., Steele V. E., Lubert R. A., Boone C. W., Covey J. M.: Strategy and planning for chemopreventive drug development: Clinical development plans II. J. Cell Biochem. 1996, 26, 54-71.
• 12. Narayan S.: Curcumin, a multi-functional chemopreventive agent, blocks growth of colon cancer cell by targeting β-catenin-mediated transactivation and cell-cell adhesion pathways. J. Mol. Histol. 2004, 35, 301-307.
• 13. Okhawa H., Ohishi N., Yagi K.: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 1979, 95, 351-358.
• 14. Piper J. T., Singhal S. S., Salameh M. S., Torman R. T., Awasthi Y. C., Awasthi S.: Mechanisms of anticarcinogenic properties of curcumin on glutathione linked detoxification enzymes in rat liver S. Int. J. Biochem. Cell Biol. 1998, 30, 445-456.
• 15. Sedlak J., Lindsay R. H.: Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s Reagent. Anal. Biochem. 1968, 25, 192-205.
• 16. Sreepriya M., Bali G.: Effects of administration of Embelin and Curcumin on lipid peroxidation, hepatic glutathione antioxidant defense and hematopoietic system during N-nitrosodiethylamine/Phenobarbital-induced hepatocarcinogenesis in Wistar rats. Mol. Cell. Biochem. 2006, 284, 49-55.