Identification of the proteins responsible for SAR DNA binding in nuclear matrix of Cucurbita pepo

• Autorzy: Rzepecki R. , Markiewicz E. , Szopa J.
• Języki publikacji: EN

Abstrakty

EN

The nuclear matrices from White bush (Cucurbita pepovar. patisonina)cell nuclei have been isolated using three methods: I, standard procedure involving extraction of cell nuclei with 2 NaCl and 1% X-100; II, the same with pre-treatment of cell nuclei with 0.5 CuS04 (stabilisation step); and III, method with extraction by lithium diiodosalicylate (LIS), and compared the polypeptide pattern. The isolated matrices specifically bind SAR DNA from human β-interferon gene in the exogenous SAR assay and in the gel mobility shift assay. Using IgG against the 32 endonuclease we have found in the DNA-protein blot assay that this protein is one of the proteins binding SAR DNA. have identified three proteins with molecular mass of 65 , 60 and 32 which are responsible for SAR DNA in the gel mobility shift assay experiments.

Słowa kluczowe

EN

extraction; White bush; DNA binding; protein; gel shift assay; nuclear matrix; DNA; beta-interferon gene; lithium diiodosalicylate; endonuclease; Cucurbita pepo

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 2
• Opis fizyczny: p.171-176,fig.

Twórcy

autor

Rzepecki R.

• Wroclaw University, S.Przybyszewskiego 63-77, 51-148 Wroclaw, Poland

autor

Markiewicz E.

autor

Szopa J.

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