Selection and activation of Escherichia coli strains for L-aspartic acid biosynthesis

• Autorzy: Papierz M , Gadomska G. , Sobierajski B. , Chmiel A.
• Języki publikacji: EN

Abstrakty

EN

The strain of Escherichia coli K-12 with high aspartase activity was irradiated with UV. After mutagenesis and selection, the mutant B-715 was isolated which was 4-times more active in L-aspartic acid biosynthesis than parental K-12 strain. The highest productivity was achieved while the strain was cultivated in the ammonium fumarate medium in 37°C for 18-30 hours. It was found that better results were obtained when before the main production step of biosynthesis of L-aspartic acid, the cells of E. coli B-715 were incubated in the activation medium with ammonium fumarate. Activation at 37°C was the most advisable for high efficiency of L-aspartic acid biosynthesis. The productivity of E. coli B-715 during 1 hour biosynthesis process was at the range 0.19-0.35 g of L-aspartic acid per 1 gram of dry mass (biomass) per minute.

Słowa kluczowe

EN

L-aspartic acid; strain selection; aspartic acid; biosynthesis; Escherichia coli

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2007
• Tom: 56
• Numer: 2
• Opis fizyczny: p.71-76,fig.,ref.

Twórcy

autor

Papierz M

• Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland

autor

Gadomska G.

autor

Sobierajski B.

autor

Chmiel A.

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