Isolation of Acanthamoeba isolates belonging to T2, T3, T4 and T7 genotypes from environmental samples in Ankara, Turkey

• Autorzy: Kilic A , Tanyuksel M , Sissons J , Jayasekera S , Khan N A
• Języki publikacji: EN

Abstrakty

EN

Acanthamoeba keratitis is a blinding infection that is becoming increasingly important in human health. Early diagnosis is a prerequisite for successful treatment and requires identification of Acanthamoeba at the genotypic level. The genus Acanthamoeba consists of both pathogenic and non-pathogenic species and has been recently classified into 13 different genotypes, T1-T12 and T14. More importantly, 95% of Acanthamoeba isolates that produce keratitis belong to T4 genotypes. In this study, we attempted to determine whether predominance of T4 isolates in Acanthamoeba keratitis is due to greater virulence or greater prevalence. We isolated 18 Acanthamoeba isolates from environmental samples in Ankara, Turkey and determined their pathogenic potential by means osmotolerance, temperature tolerance and in vitro cytotoxicity assays using corneal epithelial cells. Ribosomal DNA sequencing revealed that 10 isolates belong to T2, 5 belong to T3,2 belong to T4 and one belongs to T7 genotype. As expected, T3 and T4 isolates exhibited the most pathogenic traits and were osmotolerant, temperature tolerant and exhibited severe corneal epithelial cell cytotoxicity indicating their pathogenic potential. Overall these data indicate that high frequency of T4 isolates in keratitis cases may well be due to their greater virulence. This is the first report presenting environmental distribution of Acanthamoeba in Ankara, Turkey.

Słowa kluczowe

EN

T2 genotype; T7 genotype; Ankara; Turkey; Protozoa; keratitis; protozoan parasite; Acanthamoeba; T4 genotype; genotype; T3 genotype; granulomatous amoebic encephalitis; environmental sample

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2004
• Tom: 49
• Numer: 3
• Opis fizyczny: p.245-252,fig.,ref.

Twórcy

autor

Kilic A

• Division of Medical Parasitology, Gulhane Military Medical School of Medicine, Etlik, Ankara, Turkey

autor

Tanyuksel M

• Division of Medical Parasitology, Gulhane Military Medical School of Medicine, Etlik, Ankara, Turkey

autor

Sissons J

• School of Biological and Chemical Sciences, Birkbeck College, University of London, London, WC1E 7HX, England, U.K.

autor

Jayasekera S

• School of Biological and Chemical Sciences, Birkbeck College, University of London, London, WC1E 7HX, England, U.K.

autor

Khan N A

• School of Biological and Chemical Sciences, Birkbeck College, University of London, London, WC1E 7HX, England, U.K.

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