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2003 | 45 | 2 |

Tytuł artykułu

Regeneration of diploid and tetraploid plants of Arabidopsis thaliana via callus

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Different explants from diploid and tetraploid plants of Arabidopsis thaliana ecotype Wilna were cultured in vitro on the same medium in the same conditions. The mode and capacity for regeneration and the ploidy level of regenerants were examined in eight callus lines. The explant responses to in vitro conditions depended on the ploidy level of donor plants and on the type of explant. Callus derived from leaves of tetraploid plants showed the highest ability to regenerate. Histological investigation showed that plants regenerated mostly through organogenesis and occasionally through embryogenesis. Somatic embryos were observed more frequently in callus derived from tetraploid plants. Regenerated plants were diploids, triploids and tetraploids. The majority of regenerated plants from callus of diploid origin were diploid, but diploids were also observed quite frequently among regenerants from callus of tetraploid origin.

Wydawca

-

Rocznik

Tom

45

Numer

2

Opis fizyczny

p.145-152,fig.

Twórcy

autor
  • University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland

Bibliografia

  • Brutovská R, Čellarová E, and Doležel J. 1998. Cytogenetic variability of in vitro regenerated Hypericum performatum L. plants and their seed progenies. Plant Science 133: 221-229.
  • D’Amato F. 1986. Cytogenetics of plant cell and tissue cultures and their regenerates. CRC Critical Reviews in Plant Science 3: 73-112.
  • Fraś A. 2001. Poliploidalność w kulturze in vitro Arabidopsis thaliana. Ph.D. dissertation. University of Silesia, Katowice.
  • Fraś A, Książczyk T, Nowak K, and Małuszyńska J. 2002. The comparison of somatic embryogenesis in diploid and tetraploid plants of Arabidopsis thaliana and Crepis capillaris. XVII International Congress on Sexual Plant Reproduction, 9-13 July, Lublin, 53.
  • Gaj MD. 2001. Somatyczna embriogeneza w kulturach in vitro Arabidopsis thaliana (L.) Heynh. Wydawnictwo Uniwersytetu Śląskiego, Katowice.
  • Galbraith DW, Harkins KR, and Knapp S. 1991. Systemic endopolyploidy in Arabidopsis thaliana. Plant Physiology 96: 985-989.
  • Gamborg OL, Miller RA, and Ojima K. 1968. Nutrient requirement of suspension cultures of soybean root cells. Experimental Cell Research 50: 151-158.
  • Garcia E, and Martinez S. 1995. Somatic embryogenesis in Solanum tuberosum L. cv. Desiree from stem nodal sections. Journal Plant Physiology 145: 526-530.
  • Gottschalk W. 1988. Tetraploidy following X-radiation. Pisum Newsletters 20: 14-15.
  • Imani J, Tran Thi L, Langen G, Arnholdt-Schmitt B, Roy S, Lein C, Kumar A, and Neumann K-H. 2001. Somatic embryogenesis and DNA organisation of genomes from selected Daucus species. Plant Cell Reports 20: 537-541.
  • Jacq B, Tétu T, Sangwan RS, De Laat A, and Sangwan-Norreel BS. 1992. Plant regeneration from sugarbeet (Beta vulgaris L.) hypocotyls cultured in vitro and flow cytometric nuclear DNA analysis of regenerants. Plant Cell Reports 11: 329-333.
  • Karcz J, Weiss H, and Małuszyńska J. 2000. Structural and embryologieal studies on the diploid and tetraploid Arabidopsis thaliana genotypes, Acta Biologica Cracoviensia Series Botanica 42: 113-125.
  • Karp A. 1988. Origins and causes of chromosome instability in plant tissue culture and regeneration. Kew Chromosome Conference III. HMSO: 185-192.
  • Kasperek G. 1986. Artificial polyploids of the pea. Pisum Newsletters 18: 32.
  • Kovacs E. I. 1985. Regulation of karyotype stability in tobacco tissue cultures of normal tumorous genotypes. Theoretical and Applied Genetics 70: 548-554.
  • Kunitake H, Nakshima T, Mori K, and Tanaka M. 1998. Somaclonal and chromosomal effects of genotype, ploidy level and culture duration in Asparagus officinalis L. Euphytica 102: 309-316.
  • Larkin PJ, and Scowcroft WR. 1981. Somaclonal variation - a novel source of variability from cell culture for plant improvement. Theoretical and Applied Genetics 60: 197-214.
  • Lee M, and Phillips RL. 1988. The chromosomal basis of somaclonal variation. Annual Review in Plant Physiology and Plant Molecular Biology 39: 413-437.
  • Maluszynska J. 1990. B chromosomes of Crepis capillaris (L.) in vivo and in vitro. University of Silesia, Katowice.
  • Maluszynska J, Maluszynski M, Rebes G, and Wietrzyk E. 1990. Induced polyploids of Arabidopsis thaliana. In: Schweizer D, Peuker K, Loidl J, [eds.], Abstract of 4th International Conference on Arabidopsis Research. Vienna, University of Vienna, 12.
  • Mercy Kutty WC, and Kumar H. 1983. Studies on induced tetraploids in four diverse cultivars of pea (Pisum sativum L.). Cytologia 48: 51-58.
  • Mittelsten Scheid O, Jakovleva L, Afsar K, Maluszynska J, and Paszkowski J. 1996. A change of ploidy can modify epigenetic silencing. Proceedings of the National Academy of Sciences USA 93: 7114-7119.
  • Murashige T, and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco cultures. Plant Physiology 15: 473-492.
  • O’Neill CM, and Mathias RJ. 1993. Regeneration of plants from protoplast of Arabidopsis thaliana L. cv. Columbia (C24) via direct embryogenesis. Journal of Experimental Botany 267: 1579-1585.
  • Ono Y, Takahata Y, and Kaizuma N. 1994. Effect of genotype on shoot regeneration from cotyledonary expiants of rapeseed (Brassica napus). Plant Cell Reports 14: 13-17.
  • Patton DA, and Meinke DW. 1988. High-frequency plant regeneration from cultured cotyledons of Arabidopsis thaliana. Plant Cell Reports 10: 538-586.
  • Pillon E, Terzi M, Baldan B, Mariani P, and Schiavo FL. 1996. A protocol for obtaining embryogenic cell lines from Arabidopsis. Plant Journal 9: 573-577.
  • Rani V, Singh KP, Shran B, Nandy S, Goel S, Devarumath RM, Sreenath HL, and Raina SN. 2000. Evidence for new nuclear and mitochondrial genome organizations among high-frequency somatic embryogenesis-derived plants of allotetraploid Coffea arabica L. (Rubiaceae). Plant Cell Reports 19: 1013-1020.
  • Soltis E, and Soltis PS. 1999. Polyploidy: recurrent formation and genome evolution. Tree 14: 348-352.
  • Valvekens D, Van Montagu M, and Van Lijsbetten M. 1988. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root expiants by using kanamacin selection. Proceedings of the National Academy of Science USA 85: 5536-5540.
  • Van Den Bulk RW, Loffler HJM, Lindhout WH, and Koornneef M. 1990. Somaclonal variation in tomato: Effect of explant source and comparison with chemical mutagenesis. Theoretical and Applied Genetics 80: 817-825.
  • Wu Y, Haberland G, Zhou C, and Koop HU. 1992. Somatic embryogenesis, formation of morphogenetic callus and normal development in zygotic embryos of Arabidopsis thaliana in vitro. Protoplasma 19: 89-96.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-d3893a2d-495a-4f24-8615-ed2b2345f120
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