Translocation of polysialic acid across model membranes: Kinetic analysis and dynamic studies

• Autorzy: Janas T. , Krajinski H. , Timoszyk A. , Janas T.
• Języki publikacji: EN

Abstrakty

EN

Transmembrane translocation of polyion homopolymers takes place in the case of polyanionic polysialic acid (polySia), polyanionic polynucleotides and polycationic poly­peptides. The purpose of this work was to determine the role of membrane electrical pa­rameters on the kinetics of polyion translocation, the influence of polysialic acid on ion adsorption on positively charged membrane surface and the dynamics of the phospholipid hydrocarbon chains and choline group by using 1H-NMR. The analysis of polyion translocation was performed by using the electrical equivalent circuit of the membrane for the initial membrane potential equal to zero. The changes in polysialic acid flux was up to 75% after 1 ms in comparison with the zero-time flux. Both a decrease of membrane conductance and an increase of polyion chain length resulted in the diminution of this ef­fect. An increase of praseodymium ions adsorption to positively charged liposomes and an increase of the rate of segmental movement of the -CH2 and -CH3 groups, and the choline headgrup of lipid molecules, was observed in the presence of polySia. The results show that the direction of the vectorial polyion translocation depends both on the membrane electrical properties and the degree of polymerization of the polymer, and that polysialic acid can modulate the degree of ion adsorption and the dynamics of membrane lipids.

Słowa kluczowe

EN

K1 cell; lipid bilayer; polyanion flux; polysialic acid; membrane transport; transmembrane translocation; membrane; Escherichia coli

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 1
• Opis fizyczny: p.163-173,fig.

Twórcy

autor

Janas T.

• Technical University, Zielona Gora, Poland

autor

Krajinski H.

autor

Timoszyk A.

autor

Janas T.

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