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2005 | 52 | 4 |

Tytuł artykułu

Isolation and characterization of pigeon breast muscle cytosolic 5'-nucleotidase-I [cN-I]

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
5´-Nucleotidase specific towards dCMP and AMP was isolated from avian breast muscle and characterized. It was found to be similar to a type-I form (cN-I) identified earlier as the AMP-selective 5´-nucleotidase responsible for adenosine formation during ATP breakdown in transfected COS-7 cells. Expression pattern of the cN-I gene in pigeon tissues indicated breast muscle as a rich source of the transcript. We purified the enzyme from this source using two-step chromatography and obtained an active homogenous preparation, free of ecto-5´-nucleotidase activity. The tissue content of the activity was calculated at 0.09 U/g wet weight. The specific activity of the enzyme preparation was 4.33 U/mg protein and it preferred dCMP and AMP to dAMP and IMP as a substrate. Its kinetic properties were very similar to those of the enzyme purified earlier from heart tissue. It was strongly activated by ADP. Inhibition by inorganic phosphate was more pronounced than in heart-isolated cN-I. Despite this difference, a similar physiological function is suggested for cN-I in both types of muscle.

Wydawca

-

Rocznik

Tom

52

Numer

4

Opis fizyczny

p.789-796,fig.,ref.

Twórcy

  • Medical University of Gdansk, Gdansk, Poland
autor

Bibliografia

  • Bianchi V, Spychala J (2003) Mammalian 5´-nucleotidases. J Biol Chem 278: 46195–46198.
  • Bockman EL, McKenzie JE (1983) Tissue adenosine content in active soleus and gracilis muscles of cats. Am J Physiol 244: H552–559.
  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254.
  • Cheng B, Essackjee HC, Ballard HJ (2000) Evidence for control of adenosine metabolism in rat oxidative skeletal muscle by changes in pH. J Physiol 522: 467–477.
  • Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinum thiocyanate-phenolchloroform extraction. Anal Biochem 162: 156–159.
  • Cross HR, Murphy E, Black RG, Auchampach J, Steenbergen C (2002) Overexpression of A3 adenosine receptors decreases heart rate, preserves energetics and protects ischemic hearts. Am J Physiol 283: H1562–1568.
  • Darvish A, Metting P (1993) Purification and regulation of AMP-specific cytosolic 5´-nucleotidase from dog heart. Am J Physiol 264: H1528–1533.
  • Hellsten Y, Frandsen U (1997) Adenosine formation in contracting primary skeletal muscle cells and endothelial cells in culture. J Physiol 504: 695–704.
  • Hunsucker SA, Spychala J, Mitchell BS (2001) Human cytosolic nucleotidase I: Characterization and role in nucleoside analog resistance. J Biol Chem 276: 10498–10504.
  • Itaya K, Ui M (1966) A new micromethod for the colorimetric determination of inorganic phosphate. Clin Chim Acta 14: 361–366.
  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.
  • Marszalek J, Kostrowicki J, Spychala J (1989) LEHM: a convenient non-linear regression microcomputer program for fitting Michaelis-Menten and Hill models to enzyme kinetic data. Comput Appl Biosci 5: 239–240.
  • Sala-Newby GB, Newby AC (2001) Cloning of a mouse cytosolic 5´-nucleotidase-I identifies a new gene related to human autoimmune infertility-related protein. Biochim Biophys Acta 1521: 12–18.
  • Sala-Newby GB, Skladanowski AC, Newby AC (1999) The mechanism of adenosine formation in cells. Cloning of cytosolic 5´-nucleotidase-I. J Biol Chem 274: 17789–17793.
  • Sala-Newby GB, Freeman NVE, Skladanowski AC, Newby AC (2000) Distinct roles for recombinant cytosolic 5´-nucleotidase-I and -II in AMP and IMP catabolism in COS-7 and H9c2 rat myoblast cell lines. J Biol Chem 275: 11666–11671.
  • Sala-Newby GB, Freeman NVE, Curto MA, Newby AC (2003) Metabolic and functional consequences of cytosolic 5´-nucleotidase overexpression in neonatal rat cardiomyocytes. Amer J Physiol 285: H991–998.
  • Shryock JC, Belardinelli LB (1997) Adenosine and adenosine receptors in the cardiovascular system: biochemistry, physiology and pharmacology. Am J Cardiol 79: 2–10.
  • Skladanowski AC, Newby AC (1990) Partial purification and properties of AMP-specific soluble 5´-nucleotidase from pigeon heart. Biochem J 268: 117– 122.
  • Skladanowski AC, Smolenski RT, Tavernier M, De Jong JW, Yacoub MH, Seymour AM (1996) Soluble forms of 5´-nucleotidase in rat and human heart. Am J Physiol 210: H1493–H1500.
  • Smolenski RT, Lachno DR, Ledingham SJM, Yacoub MH (1990) Determination of sixteen nucleotides, nucleosides and bases using high-performance liquid chromatography and its application to the study of purine metabolism in hearts for transplantation. J Chromatogr 527: 414–420.
  • Truong VL, Collinson AR, Lowenstein JM (1988) 5´-Nucleotidases in rat heart. Evidence for occurrence of two soluble enzymes with different substrate specificities. Biochem J 253: 117–121.
  • Whitlock DM, Terjung RL (1987) ATP depletion in slowtwitch red muscle of rat. Am J Physiol 253: C426–432.
  • Yamazaki Y, Truong VL, Lowenstein JM (1991) 5´-Nucleotidase I from rabbit heart. Biochemistry 30: 1503–1509.
  • Zimmermann H (1992) 5´-Nucleotidase: molecular structure and functional aspects. Biochem J 285: 345–365.
  • Zimmermann H, Braun N (1999) Ecto-nucleotidases — molecular structures, catalytic properties and functional roles in the nervous system. Prog Brain Res 120: 372–38

Typ dokumentu

Bibliografia

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