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1998 | 20 | 4 |

Tytuł artykułu

Effect of nitrates and sucrose on the induction of nitrate reductase in etiolated seedling leaves from selected barley genotypes in darkness

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The parental genotypes, cv. Aramir and R567 line, as well as the selected DH lines C23, C47/1, C41 and C55, growing in darkness differed significantly in the level of NR activity in crude leaf extracts independently of nitrate concentration in the medium. The highest activity of the enzyme was found in the line C23. When plants grew on the medium with 0.5 mM KNO₃, NR activity in that genotype was almost 10-fold higher than in the parents and lines C41, C55 and also 3.5-fold higher than in the line C47/1. An increase of nitrate concentration in the medium to 10 mM caused a significant increase of NR activity in all the genotypes under study. In the line C23 this enzyme activity was only 20% lower than that found previously in the green leaves of that genotype in light. NR from the leaves of C23 and C41 lines was thermally unstable under in vitro conditions. This enzyme in the leaf extracts from the line C23 was characterized by a considerably lower unstability. The lines DH C23 and C41 growing in the dark on the medium with 0.5 mM KNO₃ did not differ in nitrate accumulation in leaves, whereas a larger nitrate content was found in the leaves of the line C41 when it grew on the medium with 10 mM KNO₃. Independently of nitrate concentration in the medium, leaves of the line C23 were found to have a higher sucrose content than those of the line C41. Excised, etiolated leaves of barley treated with 0.5 and 10 mM KNO₃ in dark under conditions favorable to transpiration had a low NR activity. Leaf treatment with a solution containing 10 mM KNO₃ + 0.2 M sucrose caused, on the average, a 13-fold increase of NR activity in comparison to leaves treated only with 10 mM KNO₃ and about a 6-fold increase of this enzyme in comparison to leaves treated with 0.5 mM KNO₃ + 0.2 M sucrose.

Wydawca

-

Rocznik

Tom

20

Numer

4

Opis fizyczny

p.363-368,fig.

Twórcy

  • Agricultural Academy of Poznan, Wolynska 35, 60-637 Poznan, Poland

Bibliografia

  • Aslam M., Oaks A. and Huffaker R.C., 1976. Effect of light and glucose on the distribution of nitrate in etiolated barley leaves. Plant Physiol. 58:588–591.
  • Aslam M. and Huffaker R.C., 1982. In vivo nitrate reduction in roots and shoots of barley (Hordeum vulgare L.) seedlings in light and darkness. Plant Physiol. 70: 1009–1013.
  • Buczek J., 1976. The role of light in the induction of nitrate reductase and nitrite reductase in cucumber seedlings. Acta Soc. Bot. Pol. XLV: 77–92.
  • Bowsher C.G., Long D.M., Oaks A. and Rothstein S.J., 1991. Effect of light/dark cycles on expression on nitrate assimilatory genes in maize shoots and roots. Plant Physiol. 95:281–285.
  • Cataldo D.A., Haroon M., Schrader L.E., Youngs V.L., 1975. Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Commun. Soil. plant Anal. 6:71–80.
  • Cheng Ch-L., Acedo G.N., Cristinsin M. and Conkling A., 1992. Sucrose mimics the light induction of Arabidopsis nitrate reductase gene transcription. Proc. Natl. Acad. Sci. 89:1861–1864.
  • Crawford N.M., 1995. Nitrate:nutrient and signal for plant growth. Plant Cell 7:859–868.
  • DeCires A., Torre A., Delgado B., Lara C., 1993. Role of light and CO2 fixation in the control of nitrate-reductase activity in barley leaves. Planta 190:277–283.
  • Gniazdowska-Skoczek H., 1997. Properties of nitrate reductase from seedling leaves of selected barley genotypes. Acta Physiol. Plant. 19:137–145.
  • Gowri G. and Campbell W.H., 1989. cDNA clones for corn leaf NADH: nitrate reductase and chloroplast NAD(P)+: Glyceraldehyd phosphate dehydrogenase. Characterization of the clones and analysis of the expression of the genes in leaves as influenced by nitrate in the light and dark. Plant Physiol. 90:792–798.
  • Hoff T., Truong H.-N. and Caboche H., 1994. The use of mutants and transgenic plants to study nitrate assimilation. Plant, Cell, Environm. 17:1251–1257.
  • Li X-Z and Oaks A., 1993. Induction and turnover of nitrate reductase in Zea mays. Influence of NO3 −. Plant Physiol. 102:1251–1257.
  • Li X-Z., Oaks A., 1994. Induction and turnover of nitrate reductase in Zea mays. Influence of light. Pant Physiol. 106:1145–1149.
  • Li X-Z., Larson D.E., Glibetic M. and Oaks A., 1995. Effect of glutamine on the induction of nitrate reductase. Physiol. Plant. 93:740–744.
  • Lillo C., 1994. Light regulation of nitrate reductase in green leaves of higher plants. Physiol. Plant. 90:616–620.
  • Mejbaum-Katzenllenbogen N., Mochnacka J., 1968. Kurs praktyczny z biochemii. PWN, WA, p. 187.
  • Redinbaught M.G. and Campbell W.H., 1991. Higher plant respones to environmental nitrate. Physiol. Plant. 82:640–650.
  • Riens B. and Heldt W., 1992. Decrease of nitrate reductase activity in spinach leaves during-dark transition. Plant Physiol. 98:573–577.
  • Sivasankar S. and Oaks A., 1995. Regulation of nitrate reductase during early seedling growth. A role for asparagine and glutamine. Plant Physiol. 107:1225–1231.
  • Skoczek H., 1992. Nitrate reductase activity and protein content in leaves and roots of two doubled haploid barley lines depending on seedling age and nitrogen source. Acta Physiol. Plant. 14:77–84.
  • Sueyoshi K., Kleinhofs A., and Warner R.L., 1995. Expression of NADH-specific and NAD(P)H-bispecific nitrate reductase genes in response to nitrate in barley. Plant Physiol. 107:1303–1311.
  • Vincentz M., Moureaux T., Leydecker M-T., Vaucheret H. and Caboche M., 1993. Regulation of nitrate and nitrite reductase expression in Nicotiana plumbaginifolia leaves by nitrogen and carbon metabolities. Pant J. 3:315–324.
  • Wray J.L. and Filner P., 1970. Structural and functional relationships of enzyme activities induced by nitrate in barley. Biochem. J. 119:715–725.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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