Characterization and expression analysis of the yellow lupin [Lupinus luteus L.] gene coding for nodule specific proline-rich protein

• Autorzy: Karlowski W. M. , Strozycki P.M. , Legocki A.B.
• Języki publikacji: EN

Abstrakty

EN

The LlPRP2 gene coding for a proline-rich protein shows a high level of similarity to, as well as significant differences from the family of ENOD2 nodule-specific genes. Several sequence motifs with putative regulatory function were identified in the 5' and 3' noncoding regions of the LlPRP2 gene. Northern blot analysis revealed that the expression of the LlPRP2 gene begins 9 days after inoculation of yellow lupin roots with Bradyrhizobium sp. (Lupinus); the expression is restricted to symbiotic nodules and is not detected in other tissues or organs. Detailed hybridization analysis showed that, when expression is activated, the LlPRP2 transcript is modified so as to produce at least three bands and a continuous distribution of decay intermediates. The modification of the LlPRP2 transcript probably involves degradation from the 5'- and/or 3'-ends of the RNA molecules. Southern blot analysis indicates that only one gene is present in the yellow lupin genome. The presence of genes homologous to the LlPRP2 gene was confirmed for three cultivars of yellow lupin and for Lupinus angustifolius. However, LlPRP2 homologues were not detected in Lupinus albus cv. Bac, indicating that this plant may lack the ENOD2 sequence.

Słowa kluczowe

EN

yellow lupin; mRNA degradation; Lupinus luteus; gene expression; plant gene; gene coding; lupin; symbiotic nitrogen fixation; expression analysis; proline-rich protein

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2000
• Tom: 47
• Numer: 2
• Opis fizyczny: p.371-383,fig.

Twórcy

autor

Karlowski W. M.

• Polish Academy of Sciences, Z.Noskowskiego 12-14, 61-704 Poznan, Poland

autor

Strozycki P.M.

autor

Legocki A.B.

Bibliografia

• 1. van Kammen, A. (1984) Suggested nomenclature for plant genes involved in nodulation and symbiosis. Plant Mol. Biol. Rep. 2, 43-45.
• 2. Schultze, M. & Kondorosi, A. (1998) Regulation of symbiotic root nodule development. Annu. Rev. Genet. 32, 33-57.
• 3. Govers, F., Nap, J.-P., van Kammen, A. & Bisseling, T. (1987) Nodulins in the developing root nodule. Plant Physiol. Biochem. 25, 309.
• 4. Golinowski, W., Kopcinska, J. & Borucki, W. (1987) The morphogenesis of lupine root nodules during infection by Rhizobium lupini. Acta Soc. Bot. Pol. 56, 687-703.
• 5. Strozycki, P.M., Karlowski, W.M. & Legocki, A.B. (1994) Lupinus luteus leghemoglobin genes structure and regulation of expression; in Proceedings of the 1st European Nitrogen Fixation Conference (Kiss, G.B. & Endre, G., eds.) pp. 342-342, Officina Press, Szeged, Hungary.
• 6. Avio, L., Sbrana, C. & Giovannetti, G. (1990) The response of different species of Lupinus to VAM endophytes. Symbiosis 9, 321-323.
• 7. O'Dell, T.E. & Trappe, J.M. (1992) Root endophytes of lupin and some other legumes in Northwestern USA. New Phytol. 122, 479-485.
• 8. Hirsch, A.M. & Kapulnik, Y. (1998) Signal transduction pathways in mycorrhizal associations: Comparisons with the Rhizobium-legume symbiosis. Fungal Genet. Biol. 23, 205-212.
• 9. Albrecht, C., Geurts, R. & Bisseling, T. (1999) Legume nodulation and mycorrhizae formation; two extremes in host specificity meet. EMBO J. 18, 281-288.
• 10. Fruehling, M., Roussel, H., Gianinazzi- Pearson, V., Puehler, A. & Perlick, A.M. (1997) The Vicia faba leghemoglobin gene VfLb29 is induced in root nodules and in roots colonized by the arbuscular mycorrhizal fungus Glomus fasciculatum. Mol. Plant Microbe Interact. 11, 393-403.
• 11. van Rhijn, P., Fang, Y., Galili, S., Shaul, O., Atzmon, N., Wininger, S., Eshed, Y., Lum, M., Li, Y., To, V., Fujishige, N., Kapulnik, Y. & Hirsch, A.M. (1997) Expression of early nodulin genes in alfalfa mycorrhizae indicates that signal transduction pathways used in forming arbuscular mycorrhizae and Rhizobium-induced nodules may be conserved. Proc. Natl. Acad. Sci. U.S.A. 94, 5467-5472.
• 12. Franssen, H.J., Nap, J.-P., Gloudemans, T., Stiekema, W., van Dam, H., Govers, F., Louwerse, J., van Kammen, A. & Bisseling, T. (1987) Characterization of cDNA for nodulin-75 of soybean: A gene product involved in early stages of root nodule development. Proc. Natl. Acad. Sci. U.S.A. 84, 4495-4499.
• 13. Franssen, H.J., Thompson, D.V., Idler, K., Kormelink, R., van Kammen, A. & Bisseling, T. (1989) Nucleotide sequence of two soybean ENOD2 early nodulin genes encoding Ngm-75. Plant Mol. Biol. 14, 103-106.
• 14. van der Wiel, C., Scheres, B., Franssen, H.J., van Lierop, M.J., van Lammeren, A., van Kammen, A. & Bisseling, T. (1990) The early nodulin transcript ENOD2 is located in the nodule parenchyma (inner cortex) of pea and soybean root nodules. EMBO J. 9, 1-7.
• 15. Dickstein, R., Bisseling, T., Reinhold, V.N. & Ausubel, F.M. (1988) Expression of nodule- specific genes in alfalfa root nodules blocked at an early stage of development. Genes Dev. 2, 677-687.
• 16. Foster, C.M., Graves, W.R. & Horner, M.A. (1998) ENOD2 cDNA clone from nodules of Maackia amurensis Rupr. & Maxim. (Accession No. AF039708) (PGR98-060). Plant Physiol. 116, 1604.
• 17. Dehio, C. & de Bruijn, F.J. (1992) The early nodulin gene SrEnod2from Sesbania rostrata is inducible by cytokinin. Plant J. 2, 117-128.
• 18. Strittmatter, G., Chia, T.-F., Trinh, T.H., Katagiri, F., Kuhlemeier, C. & Chua, N.-H. (1989) Characterization of nodule-specific cDNA clones from Sesbania rostrata and expression of the corresponding gene in stem nodules and root nodules. Mol. Plant Microbe Interact. 2, 122-127.
• 19. Szczyglowski, K. & Legocki, A.B. (1990) Isolation and nucleotide sequence of cDNA clone encoding nodule-specific (hydroxy)proline- rich protein LENOD2 from yellow lupin. Plant Mol. Biol. 15, 361-363.
• 20. Karlowski, W.M., Strozycki, P.M. & Legocki, A.B. (1997) The LlPRP2 gene from yellow lupin (Accession No. U47661) encoding nodule-specific, proline-rich protein (PGR97-049). Plant Physiol. 113, 1004.
• 21. Gloudemans, T., de Vries, S., Bussink, H., Malik, N.S.A., Franssen, H.J., Louwerse, J. & Bisseling, T. (1987) Nodulin gene expression during soybean (Glycine max) nodule development. Plant Mol. Biol. 8, 395-403.
• 22. van der Wiel, C., Norris, J.H., Bochenek, B., Dickstein, R., Bisseling, T. & Hirsch, A.M. (1990) Nodulin gene expression and ENOD2 localization in effective, nitrogen-fixing and ineffective, bacteria-free nodules of alfalfa. Plant Cell 2, 1009-1017.
• 23. Silver, D.L., Pinaev, A., Chen, R. & de Bruijn, F.J. (1996) Posttranscriptional regulation of Sesbania rostrata early nodulin gene SrEnod2by cytokinin. Plant Physiol. 112, 559-567.
• 24. Hirsch, A.M., Fang, Y., Asad, S. & Kapulnik, Y. (1997) The role of phytohormones in plant- microbe symbioses. Plant Soil 194, 171-184.
• 25. Konieczny, A., Szczyglowski, K., Boron, L., Przybylska, M. & Legocki, A.B. (1988) Expression of lupin nodulin genes during root nodule development. Plant Sci. 55, 145-149.
• 26. Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position- specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680.
• 27. Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) Basic local alignment search tool. J. Mol. Biol. 215, 403-410.
• 28. Strozycki, P.M. & Legocki, A.B. (1995) An efficient method of genomic DNA isolation from plant tissues. Acta Biochim. Polon. 42, 329- 332.
• 29. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seideman, J.G., Smith, J.A. & Struhl, K. (1991) Current Protocols in Molecular Biology. Greene & Willey-Interscience, New York.
• 30. de Vries, S., Hoge, H. & Bisseling, T. (1988) Isolation of total and polysomal RNA from plant tissues; in: Plant Molecular Biology Manual (Gelvin, S.B. et al, eds.) pp. B1-B13, Kluver Academic Publishers, Dordrecht, .
• 31. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
• 32. Stougaard, J., Sandal, N.N., Gron, A., Kuhle, A. & Marcker, K.A. (1987) 5' Analysis of the soybean leghaemoglobin lbc3 gene: Regulatory elements required for promoter activity and organ specificity. EMBO J. 6, 3565-3569.
• 33. Ramlov, K.B., Laursen, N.B., Stougaard, J. & Marcker, K.A. (1993) Site-directed mutagenesis of the organ-specific element in the soybean leghemoglobin lbc3 gene promoter. Plant J. 4, 577-580.
• 34. Strozycki, P.M., Karlowski, W.M. & Legocki, A.B. (1998) Yellow lupine gene coding for leghemoglobin I (Accession No. U50083) (PGR98-017). Plant Physiol. 116, 867.
• 35. Boron, L.J. & Legocki, A.B. (1993) Cloning and characterization of a nodule-enhanced glutamine synthetase-encoding gene from Lupinus luteus. Gene 136, 95-102.
• 36. Wilson, R.C., Long, F., Maruoka, E.M. & Cooper, J.B. (1994) A new proline-rich early nodulin from Medicago truncatula is highly expressed in nodule meristematic cells. Plant Cell 6, 1265-1275.
• 37. Kuhse, J. & Puehler, A. (1987) Conserved sequence motifs in the untranslated 3' end of leghemoglobin transcripts isolated from broad bean nodules. Plant Sci. 49, 137-143.
• 38. Wu, L., Ueda, T. & Messing, J. (1995) The formation of mRNA 3'-end in plants. Plant J. 8, 323-329.
• 39. Sikorski, M.M., Szlagowska, A.E. & Legocki, A.B. (1995) cDNA sequences encoding for two homologues of Lupinus luteus (L.) IPR-like proteins (Accession No. X79974 and X79975 for LlR18A and LlR18B mRNAs, respectively). (PGR95-114) Plant Physiol. 110, 335.
• 40. Higgs, D.C. & Colbert, J.T. (1994) Oat phytochrome A mRNA degradation appears to occur via two distinct pathways. Plant Cell 6, 1007-1019.
• 41. Gutierrez, R.A., MacIntosh, G.C. & Green, P.J. (1999) Current perspectives on mRNA stability implants: Multiple levels and mechanism of control. Trends Plant Sci. 4, 429-438.
• 42. Tanzer, M.M. & Meagher, R.B. (1995) Degradation of the soybean ribulose-1,5-bisphosphate carboxylase small-subunit mRNA, SRS4, initiates with endonucleolytic cleavage. Mol. Cell. Biol. 15, 6641-6652.
• 43. Olszewska, M.J., Karlowski, W.M. & Legocki, A.B. (1991) In situ hybridization of nodule-specific genes from yellow lupin; in Abstracts of 9th International Congress On Nitrgen Fixation, Cancun, Mexico pp. 239.
• 44. Csanadi, G., Szecsi, J., Kalo, P., Kiss, P., Endre, G., Kondorosi, A., Kondorosi, E. & Kiss, G.B. (1994) Enod12, an early nodulin gene, is not required for nodule formation and efficient nitrogen fixation in alfalfa. Plant Cell 6, 201-213.