The antioxidant properties of carnitine in vitro

• Autorzy: Solarska K , Lewinska A. , Karowicz-Bilinska A. , Bartosz G.
• Języki publikacji: EN

Abstrakty

EN

Many of the effects of carnitine are ascribed to its antioxidant properties. The aim of this study was to evaluate the antioxidant properties of carnitine in vitro. Carnitine was found to decolorize ABTS•+, and to protect fluorescein against bleaching induced by AAPH-derived peroxyl radicals and peroxynitrite, thiol groups against oxidation induced by hydrogen peroxide, peroxyl radicals, hypochlorite and peroxynitrite, and erythrocytes against hemolysis induced by peroxyl radicals and hypochlorite. These results show that carnitine has a direct antioxidant action against physiologically relevant oxidants.

Słowa kluczowe

EN

antioxidative property; carnitine; in vitro; hydrogen peroxide; hypochlorite; peroxyl; peroxynitrite; L-carnitine

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2010
• Tom: 15
• Numer: 1
• Opis fizyczny: p.90-97,fig.,ref.

Twórcy

autor

Solarska K

• University of Rzeszow, Pigonia 6, 35-959 Rzeszow, Poland

autor

Lewinska A.

autor

Karowicz-Bilinska A.

autor

Bartosz G.

Bibliografia

• 1. Benvenga, S. Effects of L-carnitine on thyroid hormone metabolism and on physical exercise tolerance. Horm. Metab. Res. 37 (2005) 566-571.
• 2. Kraemer, W.J., Volek, J.S. and Dunn-Lewis, C. L-carnitine supplementation: influence upon physiological function. Curr. Sports Med. Rep. 7 (2008) 218-223.
• 3. Brandsch, C. and Eder, K. Effect of L-carnitine on weight loss and body composition of rats fed a hypocaloric diet. Ann. Nutr. Metab. 46 (2002) 205-210.
• 4. Elmslie, J.L., Porter, R.J., Joyce, P.R., Hunt, P.J. and Mann, J.I. Carnitine does not improve weight loss outcomes in valproate-treated bipolar patients consuming an energy-restricted, low-fat diet. Bipolar Disord. 8 (2006) 503- 507.
• 5. Brandsch, C. and Eder, K. Reproductive performance of rats supplemented with L-carnitine. J. Anim. Physiol. Anim. Nutr. (Berl). 87 (2003) 301-308.
• 6. Zhou, X., Liu, F. and Zhai, S. Effect of L-carnitine and/or L-acetyl-carnitine in nutrition treatment for male infertility: a systematic review. Asia Pac. J. Clin. Nutr. 16, Suppl 1 (2007) 383-390.
• 7. De Simone, C., Tzantzoglou, S., Famularo, G., Moretti, S., Paoletti, F., Vullo, V. and Delia, S. High dose L-carnitine improves immunologic and metabolic parameters in AIDS patients. Immunopharmacol. Immunotoxicol. 15 (1993) 1-12.
• 8. Thangasamy, T., Jeyakumar, P., Sittadjody, S., Joyee, A.G. and Chinnakannu, P. L-Carnitine mediates protection against DNA damage in lymphocytes of aged rats. Biogerontology 10 (2009) 163-172.
• 9. Calabrese, V., Colombrita, C., Sultana, R., Scapagnini, G., Calvani, M., Butterfield, D.A. and Stella, A.M. Redox modulation of heat shock protein expression by acetylcarnitine in aging brain: relationship to antioxidant status and mitochondrial function. Antioxid. Redox Signal. 8 (2006) 404-416.
• 10. Calabrese, V., Cornelius, C., Mancuso, C., Barone, E., Calafato, S., Bates, T., Rizzarelli, E. and Kostova, A.T. Vitagenes, dietary antioxidants and neuroprotection in neurodegenerative diseases. Front. Biosci. 14 (2009) 376-397.
• 11. Gulcin, I. Antioxidant and antiradical activities of L-carnitine. Life Sci. 78 (2006) 803-811.
• 12. Vanella, A., Russo, A., Acquaviva, R., Campisi, A., Di Giacomo, C., Sorrenti, V. and Barcelona, M.L. L-Propionyl-carnitine as superoxide scavenger, antioxidant, and DNA cleavage protector. Cell Biol. Toxicol. 16 (2000) 99-104.
• 13. Pryor, W.A., Cueto, R., Jin, X., Koppenol, W.H., Ngu-Schwemlein, M., Squadrido, G., Uppu, P.L. and Uppu, R.M. A practical method for preparing peroxynitrite solutions of low ionic strength and free of hydrogen peroxide. Free Radic. Biol. Med. 18 (1995) 75-83.
• 14. Soszynski, M. and Bartosz, G. Effect of peroxynitrite on erythrocytes. Biochim. Biophys. Acta 1291 (1996) 107-114.
• 15. Balcerczyk, A., Sowa, K. and Bartosz, G. Metal chelators react also with reactive oxygen and nitrogen species. Biochem. Biophys. Res. Commun. 352 (2007) 522-525.
• 16. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and RiceEvans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26 (1999) 1231-1237.
• 17. Kohri, S., Fujii, H., Oowada, S., Endoh, N., Sueishi, Y., Kusakabe, M., Shimmei, M. and Kotake, Y. An oxygen radical absorbance capacity-like assay that directly quantifies the antioxidant's scavenging capacity against AAPH-derived free radicals. Anal. Biochem. 386 (2009) 167-171.
• 18. Hu, M.L., Chen, Y.K. and Lin, Y.F. The antioxidant and prooxidant activity of some B vitamins and vitamin-like compounds. Chem. Biol. Interact. 97 (1995) 63-73.
• 19. Kirsch, M. and de Groot, H. NAD(P)H, a directly operating antioxidant? FASEB J. 15 (2001) 1569-1574.