Micro-capillary liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry - a powerful tool for peptide and protein identification

• Autorzy: Ramstrom M. , Palmblad M. , Amirkhani A. , Tsybin Y.O. , Markides K.E. , Hakansson P. , Bergquist J.
• Języki publikacji: EN

Abstrakty

EN

In order to be able to study complex biological samples, a micro-capillary liquid chro­matography system was coupled to a 9.4 T Fourier transform ion cyclotron resonance mass spectrometer. The setup was tested on a tryptic digest of bovine serum albumin, which resulted in high sequence coverage (> 92 %) of the protein.

Słowa kluczowe

EN

micro-capillary liquid chromatography; protein identification; bovine serum albumin; peptide; fourier transform ion cyclotron resonance mass spectrometry

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 4
• Opis fizyczny: p.1101-1104,fig.

Twórcy

autor

Ramstrom M.

• Uppsala University, P.O. Box 531, SE-751 21 Uppsala, Sweden

autor

Palmblad M.

autor

Amirkhani A.

autor

Tsybin Y.O.

autor

Markides K.E.

autor

Hakansson P.

autor

Bergquist J.

Bibliografia

• 1.Martin, S.E., Shabanowitz, J., Hunt, D.F. & Marto, J.A. (2000), Anal. Chem. 72, 4266- 4274.
• 2.Shen, Y., Zhao, R., Belov, M.E., Conrads, T.P., Anderson, G.A., Tang, K., Pasa-Tolic, L., Veenstra, T.D., Lipton, M.S., Udseth, H.R. & Smith, R.D. (2001) Anal. Chem. 73, 1766- 1775.
• 3.Nilsson, S., Wetterhall, M., Bergquist, J., Nyholm, L. & Markides, K. (2001) Rapid Commun. Mass Spectrnm. 15, 1997-2000.
• 4. Palmblad, M., Wetterhall, M., Markides, K., Hakansson, P. & Bergquist, J. (2000) Rapid Commun. Mass Spectrnm. 14, 1029-1034.