Wplyw metisoprinolu zastosowanego in ovo na odsetek subpopulacji limfocytów T CD3 plus, CD4 plus i CD8 plus u indyków

• Autorzy: Stenzel T , Tykalowski B. , Andrzejewski M. , Koncicki A.

Warianty tytułu

EN

The influence of methisoprinol applied in ovo on the percentage of CD3 plus, CD4 plus and CD8 plus T-lymphocyte subpopulations in turkeys

• Języki publikacji: PL

Abstrakty

EN

The aim of the research was to determine the influence of a synthetic immunomodulator methisoprinol applied in ovo as a 10% solution in doses of 5 mg per egg (group I) and 20 mg of active substance per egg (group II) on the 26th day of incubation on T-lymphocyte subpopulations in the blood and spleen of 5-day-old turkeys hatched from the treated eggs. The control group (group III) were turkeys hatched from eggs in standard hatchery conditions (without in ovo injections). The percentage of T-lymphocyte subpopulations was determined by flow cytometry using specific monoclonal anti-T CD3⁺, CD4⁺ and CD8⁺antibodies and an EPICS XL apparatus. It was demonstrated that methisoprinol applied in ovo in doses of 5 mg per egg stimulated non-specific mechanisms of humoral immunity in 5-day-old turkey poults hatched from the treated eggs, which resulted in a higher percentage of CD3⁺ and CD4⁺ T-lymphocytes in their blood and spleen. Methisoprinol applied in ovo in doses of 20 mg per egg stimulated mainly non-specific mechanisms of cellular immunity in 5-day-old turkey poults hatched from the treated eggs, as evidenced by a higher percentage of CD8⁺ T-lymphocytes in the spleen.

Słowa kluczowe

PL

drob; indyki; immunomodulatory; metisoprinol; podawanie lekow; metoda in ovo; piskleta; krew; sledziona; limfocyty T; subpopulacje limfocytow; limfocyty CD3 plus; limfocyty CD4 plus; limfocyty CD8 plus

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 09
• Opis fizyczny: s.1157-1160,rys.,tab.,bibliogr.

Twórcy

autor

Stenzel T

• Uniwersytet Warminsko-Mazurski w Olsztynie, ul.Oczapowskiego 13, 10-957 Olsztyn

autor

Tykalowski B.

autor

Andrzejewski M.

autor

Koncicki A.

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