Isolation and chromosomal distribution of a novel Ty1-copia-like sequence from Secale, which enables identification of wheat-Secale africanum introgression lines

• Autorzy: Jia J , Yang Z , Li G , Liu C , Lei M , Zhang T , Zhou J , Ren Z
• Języki publikacji: EN

Abstrakty

EN

A repetitive sequence of 411 bp, named pSaO5₄₁₁, was identified in the Secale africanum genome (Rᵃ) by random amplified polymorphic DNA (RAPD) analysis of wheat and wheat-S. africanum amphiploids. GenBank BLAST search revealed that the sequence of pSaO5₄₁₁ was highly homologous to a part of a Ty1 -copia retrotransposon. Fluorescence in situ hybridization (FISH) analyses indicated that pSaO5₄₁₁ was significantly hybridized to S. africanum chromosomes of a wheat-S. africanum amphiploid, and it was dispersed along the Secale chromosome arms except the terminal regions. Basing on the sequence of pSaO5₄₁₁, a pair of sequence-characterized amplified region (SCAR) primers were designed, and the resultant SCAR marker was able to target both cultivated rye and the wild Secale species, which also enabled to identify effectively the S. africanum chromatin introduced into the wheat genome.

Słowa kluczowe

EN

isolation; chromosome distribution; Ty1-copia-like sequence; identification; wheat; Secale africanum; introgression line; retrotransposon; molecular marker

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2009
• Tom: 50
• Numer: 1
• Opis fizyczny: p.25-28,fig.,ref.

Twórcy

autor

Jia J

• School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China

autor

Yang Z

• School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China

autor

Li G

• School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China

autor

Liu C

• School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China

autor

Lei M

• School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China

autor

Zhang T

• School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China

autor

Zhou J

• School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China

autor

Ren Z

• School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China
• Key Laboratory for Plant Genetics and Breeding, Sichuan Agricultural University, Ya'an, Sichuan, China

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