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1998 | 45 | 3 |
Tytuł artykułu

Recombinant His-tagged DNA polymerase. II. Cloning and purification of Thermus aquaticus recombinant DNA polymerase [Stoffel fragment]

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Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The Stoffel DNA fragment, shortened by 12 bp from 5' end, coding for StoffelDNA polymerase (missing 4 amino acids at N-terminus of Stoffel amino-acids sequence) from the thermophilic Thermus aquaticus (strain YT-1) was amplified, cloned and expressed in Escherichia coli. The recombinant Stoffel fragment contained a polyhistidine tag at the N-terminus (21 additional amino acids) that allowed its single-step isolation by Ni2+ affinity chromatography. The enzyme was characterized and displayed high DNA polymerase activity and thermostability evidently higher than the native Tag DNA polymerase.
Wydawca
-
Rocznik
Tom
45
Numer
3
Opis fizyczny
p.661-667,fig.
Twórcy
autor
  • Technical University of Gdansk, Gdansk, Poland
autor
Bibliografia
  • 1. Erlich, H.A. (ed.) (1991) PCR Technology - Principles and Applications for DNA Amplifica­tion. W.H. Freeman, New York.
  • 2. Wright, P.A. & Wynford-Thomas, D. (1990) The polymerase chain reaction: Miracle or mi­rage? A critical review of its uses and limita­tions in diagnosis and research. J. Pathol 162, 99-117.
  • 3. Decorte. R. & Cassiman, J.J. (1993) Forensic- medicine and the polymerase chain reaction. J. Med. Genet. 30, 625-633.
  • 4. Chien, A., Edgar, D.B. & Trcla, J.M. (1976) De­oxyribonucleic acid polymerase from the ex­treme thcrmophile Themuis aquaticus. J. Bac- teriol. 127, 1550-1557.
  • 5. Mullis, K., Faloona, F., Scharf, S., Saiki, R., Horn, G. & Erlich, H. (1986) Specific enzy­matic amplification of DNA in vitro: The po­lymerase chain reaction. Cold Spring Harbor Symp. Quant. Biol. 51, 263-273.
  • 6. Arnheim, N. & Erlich, H. (1992) Polymerase chain reaction strategy. Annu. Rev. Biochem. 61. 131-156.
  • 7. Blanco, L., Bernard, A., Blasco, M.A. & Salas, M. (1991) A general structure for DNA- dependent DNA polymerases. Gene 100, 27-38.
  • 8. Braithwaite, D.K. & Ito, J. (1993) Compilation, alignment and phylogenetic relationships among DNA polymerases. Nucleic Acids Res. 21, 787-802.
  • 9. Dclarue, M.V., Pock, V., Tordo, N., Moras. D. & Argos, P. (1990) An attempt to unify the structure of polymerases. Protein Eng. 3. 461-467.
  • 10. Lawyer. F.C., Stoffel, S., Saiki, R.K., Myambo, K.. Drummod, R. & Gelfand, D.H. (1989) Isola­tion. characterization, and expression in E. coli of the DNA polymerase gene from Ther­mus aquaticus. J. Biol. Chem. 264. 6427-6437.
  • 11. Longley, M.J., Bennett. S.E. & Mosbaugh, D.W. (1990) Characterization of the 5' to 3' exonuclease associated with Thermus aquati­cus DNA polymerase. Nucleic Acids Res. 18, 7317-7322.
  • 12. Gutman, P.D. & Minton, K.W. (1993) Con­served sites in the 5'-3' exonuclease domain of Escherichia coli DNA polymerase. Nucleic Ac­ids Res. 21, 4406-4407.
  • 13. Hochuli, E., Bannwarth, W., Döbeli, H., Gentz, R. & Stüber, D. (1988) Genetic approach to fa­cilitate purification of recombinant proteins with a novel metal chelate adsorbent. Bio/ Technology 6. 1321-1325.
  • 14. Hochuli, E. (1990) Purification of recombi­nant proteins with metal chelate adsorbent. Genet Eng. (NY) 12, 87-98.
  • 15.Smirnov, Y.V., Chakhmakhcheva, O.G. & Efi- mov, V.A. (1997) Recombinant His-tagged DNA polymerase from Thermus thermophilic with reverse transcriptase activity. Rusian J. Bioorganic Chem. 23, 257-261.
  • 16. Studier, F.W. & Moffatt, B.A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol Biol 189, 113-130.
  • 17. Rubis, P., Rulka, .1., Dąbrowski, S. & Kur, J. (1996) PCR in the diagnosis of bovine leukae­mia virus infection. Bull Vet. Inst. Pulaioy 40, 85-89.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-article-d10fc41d-7b9b-43bd-9df6-c33d9ddc0fd4
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