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2008 | 57 | 1 |
Tytuł artykułu

Amplification of a single-locus variable-number direct repeats with restriction fragment length polymorphism [DR-PCR-RFLP] for genetic typing of Acinetobacter baumannii strains

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Języki publikacji
EN
Abstrakty
EN
In search of an effective DNA typing techniaue for Acinetobacter baumannii strains for hospital epidemiology use, the performance and convenience of a new target seauence was evaluated. Using known genomic seauences of Acinetobacter baumannii strains AR 319754 and ATCC 17978, we developed single-locus variable-number direct-repeat analysis using polymerase chain reaction-restriction fragment length polymorphism (DR-PCR/RFLP) method. A total of 90 Acinetobacter baumannii strains isolated from patients of the Clinical Hospital in Bydgoszcz, Poland, were examined. Initially, all strains were typed using macrorestriction analysis of the chromosomal DNA by pulsed-field gel electrophoresis (REA-PFGE). Digestion of the chromosomal DNA with the Apal endonuclease and separation of the fragments by PFGE revealed 21 uniaue types. Application of DR-PCR/RFLP resulted in recognition of 12 clusters. The results showed that the DR-PCR/RFLP method is less discriminatory than REA-PFGE, however, the novel genotyping method can be used as an alternative techniaue for generating DNA profiles in epidemiological studies of intra-species genetic relatedness of Acinetobacter baumannii strains.
Wydawca
-
Rocznik
Tom
57
Numer
1
Opis fizyczny
p.11-17,fig.,ref.
Twórcy
  • Academy of Physical Education and Sport, Kazimierza Gorskiego 1, 80-336 Gdansk, Poland
autor
autor
autor
Bibliografia
  • Al-Khoja M.S. and J.H. Darrell. 1979. The skin as the source of Acinetobacter and Moraxella species occurring in blood cultures. J. Clin. Pathol. 32: 497-499.
  • Baltimore R.S., R.L. Duncan, E.D. Shapiro and S.C. Edberg. 1989. Epidemiology of pharyngeal colonization of infants with aerobic gram-negative rod bacteria. J. Clin. Microbiol. 27: 91-95.
  • Baumann P. 1968. Isolation of Acinetobacter from soil and water. J. Bacteriol. 96: 39-42.
  • Bergogne-Berezin E. 2001. The increasing role of Acinetobacter species as nosocomial pathogens. Carr. Infect. Dis. Rep. 3: 440-444.
  • Bergogne-Berezin E. and K.J. Towner. 1996. Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin. Microbiol. Rev. 9: 148-165.
  • Bou G., G. Cervero, M.A. Dominguez, C. Quereda and J. Martinez-Beltran. 2000. PCR-based DNA fingerprinting (REP-PCR, AP-PCR) and pulsed-field gel electrophoresis characterization of a nosocomial outbreak caused by imipenem- and meropenem-resistant Acinetobacter baumannii. Clin. Microbiol. Infect. 6: 635-643.
  • Dijkshoorn L., H.M. Aucken, P. Gerner-Smidt, M.E. Kaufmann, J. Ursing and T.L. Pitt. 1993. Correlation of typing methods for Acinetobacter isolates from hospital outbreaks. J. Clin. Microbiol. 31: 702-705.
  • Dolzani L., E. Tonin, C. Lagatolla, L. Prandin and C. Monti-Bragadin. 1995. Identification of Acinetobacter isolates in the A. calcoaceticus-A. baumannii complex by restriction analysis of the 16S-23S rRNA intergenic-spacer seauences. J. Clin. Microbiol. 33: 1108-1113.
  • Ecker J.A., C. Massire, T.A. Hall, R. Ranken, T.T. Pannella, I.C. Agasino, L.B. Blyn, S.A. Hofstadler, T.P. Endy, P.T. Scott and others. 2006. Identification of Acinetobacter species and genotyping of Acinetobacter baumannii by multilocus PCR and mass spectrometry. J. Clin. Microbiol. 44: 2921-2932.
  • Gouby A., M.J. Carles-Nurit, N. Bouziges, G. Bourg, R. Mesnard and P.J. Bouvet. 1992. Use of pulsed-field gel electrophoresis for investigation of hospital outbreaks of Acinetobacter baumannii. J. Clin. Microbiol. 30: 1588-1591.
  • Huys G., M. Cnockaert, A. Nemec, L. Dijkshoorn, S. Brisse, M. Vancechoutte and J. Swings. 2005. Repetitive-DNA-element PCR fingerprinting and antibiotic resistance of pan-European multi-resistant Acinetobacter baumannii clone III strains. J. Med. Microbiol. 54: 851-856.
  • Ibrahim A., P. Gerner-Smidt and W. Liesack. 1997. Phylogenetic relationship of the twenty-one DNA groups of the genus Acinetobacter as revealed by 16S ribosomal DNA seauence analysis. Int. J. Syst. Bacteriol. 47: 837-841.
  • Koeleman J.G.M., J. Stoof, D.J. Biesmans, P.H.M. Savelkoul and C.M.J.E. Vandenbroucke-Grauls. 1998. Comparison of amplified ribosomal DNA restriction analysis, random amplified polymorphic DNA analysis, and amplified fragment length polymorphism fingerprinting for identification of Acinetobacter genomic species and typing of Acinetobacter baumannii. J. Clin. Microbiol. 36: 2522-2529.
  • Misbah S., S. AbuBakar, H. Hassan, Hanifah Y.A. and M.Y. Yusof. 2004. Antibiotic susceptibility and REP-PCR fingerprint of Acinetobacter spp. isolated from a hospital ten years apart. J. Hosp. Infect. 58: 254-61.
  • Paul, M., M. Weinberger, Y. Siegman-Igra, T. Lazarovitch, I. Ostfeld, I. Boldur, Z. Samra, H. Shula, Y. Carmeli, B. Rubinovitch and others. 2005. Acinetobacter baumannii: emergence and spread in Israeli hospitals 1997-2002. J. Hosp. Infect. 60: 256-260.
  • Rosenthal S. and I.B. Tager. 1975. Prevalence of gram-negative rods in the normal pharyngeal flora. Ann. Intern. Med. 83: 355-357.
  • Seifert H. and P. Gerner-Smidt. 1995. Comparision of ribotyping and pulsed-field gel electrophoresis for molecular typing of Acinetobacter isolates. J. Clin. Microbiol. 33: 1402-1407.
  • Seltmann G., W. Beer, H. Claus and H. Seifert. 1995. Comparative classification of Acinetobacter baumannii strains using seven different typing methods. Zentbl. Bakteriol. 282: 372-383.
  • Spence R.P., T.J. van der Reijden, L. Dijkshoorn and K.J. Towner. 2004. Comparison of Acinetobacter baumannii isolates from United Kingdom hospitals with predominant Northern European genotypes by amplified-fragment length polymorphism analysis. J. Clin. Microbiol. 42: 832-834.
  • Tenover F.C., R.D. Arbeit, R.V. Goering, P.A. Mickelsen, B.E. Murray, D.H. Persing and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33: 2233-2239.
  • Traub W.H. 1989. Acinetobacter baumannii serotyping for delineation of outbreaks of nosocomial cross-infection. J. Clin. Microbiol. 27: 2713-2716.
  • Vanecchoutte M., L. Dijkshoorn, I. Tjernberg, A. Elalchouni, P. de Vos, G. Claeys and G. Verschraegcn. 1995. Identification of Acinetobacter genomic species by amplified ribosomal DNA restriction analysis. J. Clin. Microbiol. 33: 11-15.
  • Wright M.O. 2005. Multi-resistant gram-negative organisms in Maryland: a statewide survey of resistant Acinetobacter baumannii. Am. J. Infect. Control. 33: 419-421.
  • Zapor M.J. and Moran K.A. 2005. Infectious diseases during wartime. Curr. Opin. Infect. Dis. 18: 395-359.
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Bibliografia
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