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1994 | 40 | 2 |

Tytuł artykułu

Porownanie testu aglutynacji lateksowej z konwencjonalnymi metodami diagnozowania rzesistkowicy

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Warianty tytułu

Języki publikacji

PL

Abstrakty

EN
Swabs of the genito-urinary tract of 638 women were taken and subsequently examined with the use of the latex agglutination test (LAT). The results were compared with wet mounts and cultures. The sensitivity of these methods were: 98, 30.7, and 46.1; the specifity: 99.8, 100, and 100; the usefulness: 99.5, 94.4, and 95.6, respectively. In acute trichomonosis the results of LAT and the conventional methods were almost identical but in the chronic and asymptomatic forms of the disease, the results obtained with the use of LAT were better whereas the conventional methods were of little significance. Any cross-reactivity of specific anti- Trichomonas vaginalis immunoglobulin G was observed neither between the antigens of fungi from the genus Candida nor between bacteria. Quantitative analysis of this new test indicates that the use of the latex reagent prepared in our laboratory makes it possible to detect the soluble T. vaginalis antigens in concentration of 50 ng/ml. Our results indicate that as a very sensitive and specific test, LAT can be useful in the diagnosis of asymptomatic trichomonosis when the microscopic examination and culture are ineffective.

Wydawca

-

Rocznik

Tom

40

Numer

2

Opis fizyczny

s.141-152, rys.,tab.,bibliogr.

Twórcy

autor
  • Uniwersytet Jagiellonski, ul.Czysta 18, 31-121 Krakow
autor
autor
autor

Bibliografia

  • ALDERETE J. F. 1984. Enzyme linked immunosorbent assay for detecting antibody to Trichomonas vaginalis: Use of whole cells and aqueous extract as antigen. Br. J. Vener. Dis. 60: 164-170.
  • BICKLEY L. S., KRISHER K. K., PUNSALANG A., TRUPEI M. A., REICHMAN R. C., MENEGUS M. A. 1989. Comparison of direct fluorescent antibody acridine orange wet mount and culture for detection of Trichomonas vaginalis in women attending a public sexually transmited diseases clinic. Sex. Trans. Dis. 16: 127-131.
  • CARNEY J. A., UNADKAT P., YULE A., RAJAKUMAR R., LACEY C. J. N., ACKERS J. P. 1988. New rapid latex agglutination test for diagnosing Trichomonas vaginalis infection. J. Clin. Pathol. 41: 806-808.
  • DIERKSHEIDE W. C. 1987. Medical decisions interpreting clinical tests. A. S. M. News. 53: 677-680.
  • FOUTS A. C., KRAUS S. J. 1980. Trichomonas vaginalis: reevaluation ofits clinical presentation and laboratory diagnosis. J. Infect. Dis. 141: 137-143.
  • GOMBOSOVA A., VALENT M. 1990. Dot-immunobinding assay with monoclonal antibody for detection of Trichomonas vaginalis in clinical specimens. Genitourin. Med. 66: 447-450.
  • HUME J. C. 1983. Trichomoniasis, candidiasis and Gardnerella vaginalis as sexually transmitted diseases. Dermatol. Clin. 1: 137-151.
  • KAŁUŻEWSKI S., JAGIELSKI M., SZYCH M., ZALEWSKA M., TOMASZEWSKA A. 1988. Ocena przydatności odczynu lateksowego do szybkiego wykrywania 0 antygenów pałeczek Salmonella w płynach hodowli bakteryjnych i próbkach materiału klinicznego. Med. Dośw. Mikrobiol. 40: 198-205.
  • KRIEGER J. N., TAM M. R., STEVENS C. E., NIELSEN I. O., HALE J., KIVIAT N. B. HOLMES K. K. 1988. Diagnosis of trichomoniasis: comparison of conventional wet mount examination with cytologie studies, cultures, and monoclonal antibody staining of direct specimens. J. Am. Med. Assoc. 259: 1223-1227.
  • LEVETT P. N. 1980. A comparison of five methods for the detection of Trichomonas vaginalis in clinical specimens. Med. Lab. Sci. 37: 85-88.
  • LISI P. I., DONDERO R. S., KWIATKOSKI D., SPNENCE M., REIN M. F., ALDERETE I. F. 1988. Monoclonal-antibody-based enzyme-linked immunosorbent assay for Trichomonas vaginalis. J. Clin. Microbiol. 26: 1684-1686.
  • MCKINNEY M. M., PARKINSON A. 1987. A simple, nonchromatographic procedure to purify immunoglobulins from serum and ascites fluid. J. lmmunol. Methods. 96: 271-278.
  • PASTUSZAK I. 1970. Odpowiedź immunologiczna zwierząt uodparnianych antygenami Trichomonas vaginalis. Praca magisterska. Akademia Medyczna, Kraków.
  • SPENCE M. R., HOLLANDER D. H., SMITH I., MCCAIG L., SEWELL D., BROCKMAN M. 1980. The clinical and laboratory diagnosis of Trichomonas vaginalis infection. Sex. Transm. Dis. 7: 168-171.
  • WATT R. M., PHILIP A., WOS S. M., SAM G. I. 1986. Rapid assay for immunological detection of Trichomonas vaginalis. J. Clin. Microbiol. 24: 551-555.
  • YULE A., GELLAN M. C. A., ORIEL I. D., ACKERS J. P. 1987. Detection of Trichomonas vaginalis antigen in women by enzyme immunoassay. J. Clin. Pathol. 40: 566-568.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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