Slime production by Staphylococcus aureus strains isolated from cases of bovine mastitis

• Autorzy: Krukowski H , Szymankiewicz M. , Lisowski A.
• Języki publikacji: EN

Abstrakty

EN

The objective of the present research was to determine the frequency of slime production by Staphylococcus aureus strains recovered from bovine mastitis and comparison of slime formation frequency, depending on the determination procedure employed. The investigations embraced 59 Staphylococcus aureus strains obtained from the inflammatory secretion of mammary glands of 45 cows. Slime production was determined using Christensen method and Congo red agar (CRA) method. Out of the 59 S. aureus isolates, 47.45% produced slime as shown by Christensen method and 42.37% by the CRA method. However, 7 strains (11.86%) demonstrated slime production ability only when tested by the Christensen method, whereas 4 strains (6.77%) only using the CRA method.

Słowa kluczowe

EN

slime formation frequency; mammary gland inflammation; Christensen method; mastitis; slime production; determination procedure; cow; Staphylococcus aureus

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2008
• Tom: 57
• Numer: 3
• Opis fizyczny: p.253-255,ref.

Twórcy

autor

Krukowski H

• University of Life Sciences, Akademicka 13, 20-950 Lublin, Poland

autor

Szymankiewicz M.

autor

Lisowski A.

Bibliografia

• Baselga R., I. Albizu, M. de la Cruz, E. del Cacho, M. Barberan and B. Amorena. 1993. Phase variation of slime production in Staphylococcus aureus: Implication in colonization and virulence. Infect. Immun. 61: 4857-4862.
• Christensen G.D, W.A. Simpson, J.J. Younger, L.M. Baddour, F.F. Barrett, D.M. Melton and E.H. Beachey. 1985. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J. Clin. Microbiol. 22: 996-1006.
• Christensen G.D., W.A. Simpson, A.L. Bisno and E.H. Beachey. 1982. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect. Immun. 37: 318-326.
• Cucarella C, C. Solano, J. Valle, B. Amorena, I. Lasa and J.R. Penadé. 2001. Bap, a Staphylococcus aureus surface protein involved in biofilm formation. J. Bacteriol. 183: 2888-2896.
• Cucarella C, M.A. Tormo, C. Ubeda, M.P. Trotonda, M. Monzón, C. Peris, B. Amorena, I. Lasa and J. R. Penadé. 2004. Role of biofilm-associated protein Bap in the pathogenesis of bovine Staphylococcus aureus. Infect. Immun. 72: 2177-2185.
• Dorocka-Bobkowska B. and K. Konopka. 2003. Biofilm formation by Candida and its role in the pathogenesis of chronic infections - review (in Polish). Dent. Med. Probl. 40: 405-410.
• Fox L.K., R.N. Zadoks and C.T. Gaskins. 2005. Biofilm production by Staphylococcus aureus associated with intramammary infection. Vet. Microbiol. 107: 295-299.
• Freeman D.J., F.R. Falkiner and C.T. Keane. 1989. New method for detecting slime production by coagulase negative staphylococci. J. Clin. Pathol. 42: 872-874.
• Gad F., T. Zahra, T. Hasan and M.R. Hamblin. 2004. Effects of growth phase and extracellular slime on photodynamic inactivation of gram-positive pathogenic bacteria. Antimicrob. Agents Chemother. 48: 2173-2178.
• Górski N.P. and J.N. Palmer. 2007. Bacterial biofilms in chronic rhinosinusitis (in Polish). Mag. Otolaryngologiczny (Special edition), February - 2007: 3-12.
• Melchior M.B., J. Fink-Gremmels and W. Gaastra. 2006 Comparative assessment of the antimicrobial susceptibility of Staphylococcus aureus isolates from bovine mastitis in biofilm versus planktonie culture. J. Vet. Med. B. 53: 326-332.
• Melchior M.B., H. Vaarkamp and J. Fink-Gremmels. 2006. Biofilms - A role in recurrent mastitis infections? Vet. J. 171: 398-407.
• Nickerson S.C. 1993. Eliminating chronic Staphylococcus aureus mastitis. Vet. Med. 88, 375-381.
• Nickerson S.C. 1993. Preventing new Staphylococcus aureus mastitis infections. Vet. Med. 88, 368-374.
• Oliveira M., R. Bexiga, S.F. Nunes, C. Carneiro, L.M. Cavaco, F. Bernardo and C.L. Vilela. 2006. Biofilm-forming ability profiling of Staphylococcus aureus and Staphylococcus epidermidis mastitis isolates. Vet. Microbiol. 118: 133-140.
• Szymankiewicz M., G. Janicka, E. Sieradzka and D. Krawiecka. 2002. Influence of the medium on the ability to slime production by strains of selected species of Candida (in Polish). Mikol. Lek. 9: 97-100.
• Szymankiewicz M., G. Janicka and J. Wróblewska. 2004. Ability of slime production of Staphylococcus aureus strains obtained from clinical materials (in Polish). Pol. Merk. Lek. 17: 338-340.
• Vasudevan P., M.K.M. Nair, T. Annamalai and K.S. Venkitanarayanan. 2003. Phenoptypic and genotypie characterization of bovine mastitis isolates Staphylococcus aureus for biofilm formation. Vet. Microbiol. 92: 179-185.